bacteroides heparinase ii (New England Biolabs)

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New England Biolabs bacteroides heparinase ii
Bacteroides Heparinase Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/heparinase/Bacteroides+Heparinase+II/pmc09319433-123-2-13
Average 94 stars, based on 40 article reviews

bacteroides heparinase ii - by Bioz Stars, 2026-09

94/100 stars

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Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pCMV6-XL4-CD32A Origene Cat#SC112914 pCMV6-XL5-CD32B Origene Cat#SC128159 pCMV6-XL5-CD32C Origene Cat#SC124933 pCMV-CD64A-GFP Origene Cat#RG207487 pCMV-CD16A-GFP Origene Cat#RG219204 pBK-CMV-FynN18-GFP O.T.

Concentration Assay:

Article Title: A tumor-associated heparan sulfate-related glycosaminoglycan promotes the generation of functional regulatory T cells
Article Snippet: .. In some experiments, the samples to be tested for Ca10 were previously subjected to enzymatic treatment: (i) Bacteroides heparinase II plus heparinase III (New England Biolabs) prepared in heparinase reaction buffer at final concentrations of 85 AU/mL and 14.8 AU/mL, respectively, for 24 h at 30 °C; (ii) Streptomyces griseus Pronase (Roche) prepared in 10 mM CaCl 2 at a final concentration of 0.2 mg/mL for 24 h at 40 °C; and (iii) Sialidase 33 A (Nzytech) prepared in reaction buffer at a final concentration of 0.375 μg/mL. ..

Article Title: A tumor-associated heparan sulfate-related glycosaminoglycan promotes the generation of functional regulatory T cells.
Article Snippet: .. In some experiments, the samples to be tested for Ca10 were previously subjected to enzymatic treatment: (i) Bacteroides heparinase II plus heparinase III (New England Biolabs) prepared in heparinase reaction buffer at final concentrations of 85 AU/mL and 14.8 AU/mL, respectively, for 24 h at 30 °C; (ii) Streptomyces griseus Cellular & Molecular Immunology (2023) 20:1499 – 1512 Pronase (Roche) prepared in 10mM CaCl2 at a final concentration of 0.2 mg/mL for 24 h at 40 °C; and (iii) Sialidase 33 A (Nzytech) prepared in reaction buffer at a final concentration of 0.375 μg/mL. ..

Virus:

Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells.
Article Snippet: For all HIV-1 binding assays the culture medium was CO2-independent medium (Thermo Fischer Scientific). .. The enzymatic digestion with 1 U/ml of Heparinase I/II/III (Heparinase I [Cat. No. P0735S]; Heparinase II [Cat. No. P0736S]; Heparinase III [Cat. No. P0737S]; New England Biolabs) and Chondroitinase (Chondroitinase ABC from Proteus vulgaris [Cat. No. C3667-5UN] Merck) was performed for 15 min at 37 C in PBS stopping the reaction by addition of medium supplemented with 10% FBS followed by a washing step with PBS, prior to virus inoculation. .. For all HIV-1 binding assays the culture medium was CO2-independent medium (Thermo Fischer Scientific).

Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells
Article Snippet: 344602] clone SK3, Biolegend; [Cat. No.555344] clone RPA-T4, BD; [Cat. No. 556614] clone M-T477, BD; [Cat. No. 550625] clone L200, BD; α-CD11b [Cat. No. sc-59744] clone VIM12, Santa Cruz Biotechnology; [Cat. No. 301361] clone ICRF44, Biolegend; α-CD11c [Cat. No. 337202] clone Bu15, Biolegend; α-CD206 [Cat. No.555953] clone 19.2, BD; [Cat. No. 321149] clone 15-2, Biolegend; α-CD209 [Cat. No. 30835-MSM1-P1ABX] clone C209, 1781, Thermo Fischer Scientific, [Cat. No. MAB16211], R&D System; α-CD13 [Cat. No. 301723] clone WM15, Biolegend; α-CD226 [Cat. No. 337102] clone TX25, Biolegend; α-CD74 [Cat. No. 326802] clone LN2, Biolegend; α-CD54 [Cat. No 0.322721] clone HCD54, Biolegend; α-CD227 [Cat. No. 355602] clone 16A, Biolegend; α-HLA-DR [Cat. No. 307665] clone L243, Biolegend; Biolegend; mouse IgG1κ [Cat. No. 400102], Biolegend; mouse IgG2a [Cat. No. 401501], Biolegend; mouse IgG2b [Cat. No. 400302], Biolegend. .. The enzymatic digestion with 1 U/ml of Heparinase I/II/III (Heparinase I [Cat. No. P0735S]; Heparinase II [Cat. No. P0736S]; Heparinase III [Cat. No. P0737S]; New England Biolabs) and Chondroitinase (Chondroitinase ABC from Proteus vulgaris [Cat. No. C3667-5UN] Merck) was performed for 15 min at 37°C in PBS stopping the reaction by addition of medium supplemented with 10% FBS followed by a washing step with PBS, prior to virus inoculation. .. For all HIV-1 binding assays the culture medium was CO 2 -independent medium (Thermo Fischer Scientific).

Incubation:

Article Title: Enhanced anticancer efficacy of primed natural killer cells via coacervate-mediated exogenous interleukin-15 delivery.
Article Snippet: Effective exogenous delivery of interleukin (IL)-15 to natural killer (NK) cells with subsequent anticancer efficacy could be a promising immune cell-based cancer immunotherapy.. For the protection of encapsulated cargo IL-15 while maintaining its bioactivity under physiological conditions, we utilized a coacervate (Coa) consisting of a cationic methoxy polyethylene glycol–poly(ethylene arginyl aspartate diglyceride) (mPEG–PEAD) polymer, anionic counterpart heparin, and cargo IL-15. mPEGylation into the backbone cation effectively preserved the colloidal stability of Coa in harsh environments and enhanced the protection of cargo IL-15 than normal Coa without mPEGylation.. Proliferation and anticancer efficacy of primed NK cells through co-culture with multiple cancer cell lines were enhanced in the mPEG–Coa group due to the maintained bioactivity of cargo IL-15 during the ex vivo expansion of NK cells.

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