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heparinases  (R&D Systems)


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    Structured Review

    R&D Systems heparinases
    Heparinases, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/heparinase/Recombinant+F%2E+heparinum+Heparinase+I+Protein%2C+CF/pm39048821-570-32-35
    Average 94 stars, based on 6 article reviews
    heparinases - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Confocal Microscopy:

    Article Title: Shifts in receptors during submergence of an encephalitic arbovirus.
    Article Snippet: .. Confocal microscopy with fluorescently labelled VLPs A total of 107 K562 cells stably expressing human PCDH10 or MXRA8 were centrifuged at 1,000 rpm for 5 min and resuspended in a mixture of heparinases (heparinase I (R&D Systems 7897-GH) at 2 units ml−1, heparinase II (Sigma H8891) at 1 unit ml−1, heparinase III (R&D Systems 6145-GH) at 2 units ml−1). ..

    Stable Transfection:

    Article Title: Shifts in receptors during submergence of an encephalitic arbovirus.
    Article Snippet: .. Confocal microscopy with fluorescently labelled VLPs A total of 107 K562 cells stably expressing human PCDH10 or MXRA8 were centrifuged at 1,000 rpm for 5 min and resuspended in a mixture of heparinases (heparinase I (R&D Systems 7897-GH) at 2 units ml−1, heparinase II (Sigma H8891) at 1 unit ml−1, heparinase III (R&D Systems 6145-GH) at 2 units ml−1). ..

    Expressing:

    Article Title: Shifts in receptors during submergence of an encephalitic arbovirus.
    Article Snippet: .. Confocal microscopy with fluorescently labelled VLPs A total of 107 K562 cells stably expressing human PCDH10 or MXRA8 were centrifuged at 1,000 rpm for 5 min and resuspended in a mixture of heparinases (heparinase I (R&D Systems 7897-GH) at 2 units ml−1, heparinase II (Sigma H8891) at 1 unit ml−1, heparinase III (R&D Systems 6145-GH) at 2 units ml−1). ..

    Recombinant:

    Article Title: Targeting chondroitin sulfate suppresses macropinocytosis of breast cancer cells by modulating syndecan-1 expression.
    Article Snippet: Full-length Chpf cDNA-pCMV6 plasmid was purchased from OriGene (MR216423; Rockville, MD, USA). .. Recombinant Heparinase I, II, and III (Catalog #: 7897-GH, 6336-GH, and 6145-GH) were purchased from R&D Systems (Minneapolis, MN, USA). .. Antibody against Syndecan 1 (D4Y7H; Cell Signaling, Danvers, MA, USA) was used for western blots.

    Article Title: Optimizing miR-29 Measurements in Biobanked, Heparinized Samples
    Article Snippet: .. Three types of heparinases and two individual manufacturers were tested; recombinant F. Heparinum heparinase I (E. coli derived, #7897-GH-010, R&D systems Minneapolis, MN, USA); recombinant P. heparinus heparinase II (E. coli derived, #6336-GH-1010, R&D systems Minneapolis, MN, USA); recombinant P. heparinus heparinase III (E. coli derived, #6145-GH-1010, R&D systems Minneapolis, MN, USA) and heparinase I (B. efferthii-derived, #P0735S, New England BioLabs, Ipswich, MA, USA). .. Human samples, collected in heparin tubes (6.0 mL BD Vacutainer lithium heparin tubes containing 95 USP units) were divided into nine aliquots and treated with the above heparinases.

    Article Title: SSSMuG: Same Sample Sequential Multi-Glycomics.
    Article Snippet: The mammalian glycome is structurally complex and diverse, composed of many glycan classes such as Nand Olinked glycans, glycosaminoglycans (GAGs), glycosphingolipids (GSLs), and other distinct glycan features such as polysialic acids (PolySia), sulfation, and proteoglycan attachment stubs.. Various methods are used to analyze these different components of the glycome, but they require prefractionated/partitioned samples to target each glycan class individually.. To address this need for a knowledge of the relationship between the different glycan components of a biological system, we developed a sequential release workflow for analysis of multiple conjugated glycan classes (PolySia, GAGs, GSL glycans, N-glycans, and O-glycans) from the same tissue lysate, termed SSSMuG�Same Sample Sequential Multi-Glycomics.

    Article Title: Targeting chondroitin sulfate suppresses macropinocytosis of breast cancer cells by modulating syndecan‐1 expression
    Article Snippet: Full‐length Chpf cDNA‐pCMV6 plasmid was purchased from OriGene (MR216423; Rockville, MD, USA). .. Recombinant Heparinase I, II, and III (Catalog #: 7897‐GH, 6336‐GH, and 6145‐GH) were purchased from R&D Systems (Minneapolis, MN, USA). .. Antibody against Syndecan 1 (D4Y7H; Cell Signaling, Danvers, MA, USA) was used for western blots.

    Article Title: Beware, commercial chondroitinases vary in activity and substrate specificity.
    Article Snippet: IBEX and R&D Systems sell ChAC and ChB. .. Whereas IBEX purify their chondroitinases from F. heparinum, R&D Systems produces recombinant F. heparinum chondroitinases in E. coli (Table I). ..

    Derivative Assay:

    Article Title: Optimizing miR-29 Measurements in Biobanked, Heparinized Samples
    Article Snippet: .. Three types of heparinases and two individual manufacturers were tested; recombinant F. Heparinum heparinase I (E. coli derived, #7897-GH-010, R&D systems Minneapolis, MN, USA); recombinant P. heparinus heparinase II (E. coli derived, #6336-GH-1010, R&D systems Minneapolis, MN, USA); recombinant P. heparinus heparinase III (E. coli derived, #6145-GH-1010, R&D systems Minneapolis, MN, USA) and heparinase I (B. efferthii-derived, #P0735S, New England BioLabs, Ipswich, MA, USA). .. Human samples, collected in heparin tubes (6.0 mL BD Vacutainer lithium heparin tubes containing 95 USP units) were divided into nine aliquots and treated with the above heparinases.



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