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recombinant pedobacter heparinus heparinase iii  (R&D Systems)


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    Structured Review

    R&D Systems recombinant pedobacter heparinus heparinase iii
    Recombinant Pedobacter Heparinus Heparinase Iii, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/heparinase/Recombinant+P%2E+heparinus+Heparinase+III+Protein%2C+CF/pm41660839-95-19-27
    Average 94 stars, based on 9 article reviews
    recombinant pedobacter heparinus heparinase iii - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Generated:

    Article Title: Sulfated motifs in heparan sulfate inhibit Streptococcus pneumoniae adhesion onto fibronectin and attenuate corneal infection
    Article Snippet: RNeasy Plus Mini Kit was from Qiagen. .. Heparinase I-treated HS and heparinase III-treated HS were generated by heparinase I and heparinase III (R&D System) digestion of HS, respectively. ..

    Recombinant:

    Article Title: Elevated vaginal heparan sulfate correlates with impaired neutrophil killing of Candida albicans in women with vulvovaginal candidiasis
    Article Snippet: To test inhibitory effects of HS, control (non-inhibitory) VCM was spiked with purified HS (500 μg/mL, from porcine intestine, AMSBIO) prior to coculture. .. To degrade HS, symptomatic (inhibitory) VCM was pretreated with either recombinant human HPSE (rhHPSE, 4 μg/mL, R&D Systems) or recombinant Pedobacter heparinus heparinase III (r Ph HPSE, 3 ng/mL, R&D Systems) for 1 h at 37°C. ..

    Article Title: Glycosaminoglycan modifications of betaglycan regulate ectodomain shedding to fine-tune TGF-β signaling responses in ovarian cancer.
    Article Snippet: .. Human TGFbeta RIII DuoSet ELISA (catalog DY242), DuoSet ELISA Ancillary Kit (catalog DY008), Recombinant Human TGF-beta RIII Protein (catalog 242-R3), Heparinase III (catalog 6145-GH-010) and Chondroitinase ABC (catalog No. C3667) were obtained from Sigma-Aldrich, and recombinant TGF-β1, and TGF-β2, were purchased from R&D Systems. .. A83-01 (catalog 100–1041, 72,024) was purchased from Stemcell Technologies.

    Article Title: Canonical BMP-Smad signalling promotes neurite growth in rat midbrain dopaminergic neurons.
    Article Snippet: Ventral midbrain (VM) dopaminergic (DA) neurons project to the dorsal striatum via the nigrostriatal pathway to regulate voluntary movements, and their loss leads to the motor dysfunction seen in Parkinson’s disease (PD).. Despite recent progress in the understanding of VM DA neurogenesis, the factors regulating nigrostriatal pathway development remain largely unknown.. The bone morphogenetic protein (BMP) family regulates neurite growth in the developing nervous system and may contribute to nigrostriatal pathway development.

    Article Title: Elevated vaginal heparan sulfate correlates with impaired neutrophil killing of Candida albicans in women with vulvovaginal candidiasis.
    Article Snippet: To test inhibitory effects of HS, control (non-inhibitory) VCM was spiked with purified HS (500 μg/mL, from porcine intestine, AMSBIO) prior to coculture. .. To degrade HS, symptomatic (inhibitory) VCM was pretreated with either recombinant human HPSE (rhHPSE, 4 μg/mL, R&D Systems) or recombinant Pedobacter heparinus heparinase III (rPhHPSE, 3 ng/mL, R&D Systems) for 1 h at 37°C. ..

    Article Title: Optimizing miR-29 Measurements in Biobanked, Heparinized Samples
    Article Snippet: .. Three types of heparinases and two individual manufacturers were tested; recombinant F. Heparinum heparinase I (E. coli derived, #7897-GH-010, R&D systems Minneapolis, MN, USA); recombinant P. heparinus heparinase II (E. coli derived, #6336-GH-1010, R&D systems Minneapolis, MN, USA); recombinant P. heparinus heparinase III (E. coli derived, #6145-GH-1010, R&D systems Minneapolis, MN, USA) and heparinase I (B. efferthii-derived, #P0735S, New England BioLabs, Ipswich, MA, USA). .. Human samples, collected in heparin tubes (6.0 mL BD Vacutainer lithium heparin tubes containing 95 USP units) were divided into nine aliquots and treated with the above heparinases.

    Article Title: SSSMuG: Same Sample Sequential Multi-Glycomics.
    Article Snippet: The mammalian glycome is structurally complex and diverse, composed of many glycan classes such as Nand Olinked glycans, glycosaminoglycans (GAGs), glycosphingolipids (GSLs), and other distinct glycan features such as polysialic acids (PolySia), sulfation, and proteoglycan attachment stubs.. Various methods are used to analyze these different components of the glycome, but they require prefractionated/partitioned samples to target each glycan class individually.. To address this need for a knowledge of the relationship between the different glycan components of a biological system, we developed a sequential release workflow for analysis of multiple conjugated glycan classes (PolySia, GAGs, GSL glycans, N-glycans, and O-glycans) from the same tissue lysate, termed SSSMuG�Same Sample Sequential Multi-Glycomics.

    Confocal Microscopy:

    Article Title: Shifts in receptors during submergence of an encephalitic arbovirus.
    Article Snippet: .. Confocal microscopy with fluorescently labelled VLPs A total of 107 K562 cells stably expressing human PCDH10 or MXRA8 were centrifuged at 1,000 rpm for 5 min and resuspended in a mixture of heparinases (heparinase I (R&D Systems 7897-GH) at 2 units ml−1, heparinase II (Sigma H8891) at 1 unit ml−1, heparinase III (R&D Systems 6145-GH) at 2 units ml−1). ..

    Stable Transfection:

    Article Title: Shifts in receptors during submergence of an encephalitic arbovirus.
    Article Snippet: .. Confocal microscopy with fluorescently labelled VLPs A total of 107 K562 cells stably expressing human PCDH10 or MXRA8 were centrifuged at 1,000 rpm for 5 min and resuspended in a mixture of heparinases (heparinase I (R&D Systems 7897-GH) at 2 units ml−1, heparinase II (Sigma H8891) at 1 unit ml−1, heparinase III (R&D Systems 6145-GH) at 2 units ml−1). ..

    Expressing:

    Article Title: Shifts in receptors during submergence of an encephalitic arbovirus.
    Article Snippet: .. Confocal microscopy with fluorescently labelled VLPs A total of 107 K562 cells stably expressing human PCDH10 or MXRA8 were centrifuged at 1,000 rpm for 5 min and resuspended in a mixture of heparinases (heparinase I (R&D Systems 7897-GH) at 2 units ml−1, heparinase II (Sigma H8891) at 1 unit ml−1, heparinase III (R&D Systems 6145-GH) at 2 units ml−1). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Glycosaminoglycan modifications of betaglycan regulate ectodomain shedding to fine-tune TGF-β signaling responses in ovarian cancer.
    Article Snippet: .. Human TGFbeta RIII DuoSet ELISA (catalog DY242), DuoSet ELISA Ancillary Kit (catalog DY008), Recombinant Human TGF-beta RIII Protein (catalog 242-R3), Heparinase III (catalog 6145-GH-010) and Chondroitinase ABC (catalog No. C3667) were obtained from Sigma-Aldrich, and recombinant TGF-β1, and TGF-β2, were purchased from R&D Systems. .. A83-01 (catalog 100–1041, 72,024) was purchased from Stemcell Technologies.

    Derivative Assay:

    Article Title: Optimizing miR-29 Measurements in Biobanked, Heparinized Samples
    Article Snippet: .. Three types of heparinases and two individual manufacturers were tested; recombinant F. Heparinum heparinase I (E. coli derived, #7897-GH-010, R&D systems Minneapolis, MN, USA); recombinant P. heparinus heparinase II (E. coli derived, #6336-GH-1010, R&D systems Minneapolis, MN, USA); recombinant P. heparinus heparinase III (E. coli derived, #6145-GH-1010, R&D systems Minneapolis, MN, USA) and heparinase I (B. efferthii-derived, #P0735S, New England BioLabs, Ipswich, MA, USA). .. Human samples, collected in heparin tubes (6.0 mL BD Vacutainer lithium heparin tubes containing 95 USP units) were divided into nine aliquots and treated with the above heparinases.



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