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Structured Review

Promega go script reverse kit
Go Script Reverse Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript/goscript+reverse+kit/pmc09421433-171-11-14
Average 90 stars, based on 1 article reviews
go script reverse kit - by Bioz Stars, 2026-09
90/100 stars

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Construct:

Article Title: Methyl-CpG binding proteins (MBD) family evolution and conservation in plants.
Article Snippet: .. The cDNA was constructed using 4 μg of RNA using the GoScriptTM Reverse kit (Promega). .. To analyze gene expression, RT-qPCR reactions were performed with SYBR Green PCR Master Mix (Applied Biosystems).

Real-time Polymerase Chain Reaction:

Article Title: GLI1 and AXIN2 Are Distinctive Markers of Human Calvarial Mesenchymal Stromal Cells in Nonsyndromic Craniosynostosis
Article Snippet: RNA was quantified using a UV spectrophotometer (DU 800 Beckman Coulter, Brea, CA, USA). .. Five hundred nanograms of total RNA were used as template for retrotranscription reaction by GoScript Reverse Kit (Promega, San Luis Obispo, CA, USA) according to the manufacturer’s protocol. cDNA was used as template for Quantitative Real Time PCR analysis (qPCR). .. The differential expression of AXIN2 , GLI1 , THY1 (also known as CD90), ITGAV , TEK and ENPEP was validated in N- and P-CMSC and in BM-MSC cells. qPCR was also exploited to analyse the expression of selected osteogenic genes as Runt-related transcription factor 2 ( RUNX2 ), Alkaline phosphatase ( ALP ), Osteonectin ( ON ), Osteopontin ( OPN ) and Osteocalcin ( OCN ). qPCR was performed using Syber Green master mix (GoTaq qPCR Master Mix Kit, Promega, San Luis Obispo, CA, USA).

Article Title: Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients.
Article Snippet: RNA quantity was assessed using the Nanodrop One spectrophotometer (Thermo Fischer Scientific). .. Up to 500 ng of total RNA were reverse transcribed using the GoScriptTM Reverse Kit (Promega, San Luis Obispo, CA, USA) and the expression of selected mesenchymal, adipogenic and fibrogenic genes was quantified by real-time quantitative PCR (qRT-PCR) with Syber Green master mix (GoTaq qPCR Master Mix Kit, Promega), and custom sequence-specific oligonucleotide primer pairs (listed in Supplementary Table S2). ..

Article Title: Targeted allele-specific FGFR2 knockdown via human recombinant ferritin nanoparticles for personalized treatment of Crouzon syndrome
Article Snippet: Isolated RNA was quantified using a UV spectrophotometer (NanoDrop One/OneC Microvolume UV-Vis, Thermo Scientific). .. Then, up to 500 ng/μL of total RNA of each sample were reverse transcribed into cDNA using GoScript Reverse Kit (Promega) following the manufacturer’s protocol. qPCR of above-mentioned genes was performed using GoTaq(R) qPCR Master Mix (Promega) and TaqMan Universal PCR Master Mix (Thermo Fisher Scientific). .. Specifically, sequence-specific fluorescent oligonucleotide Taqman probes and primers (Thermo Fisher Scientific) were designed to evaluate the expression levels of wild-type and mutant FGFR2 transcripts in CS patient cells treated with siRNAs and with HFT-HIS-PASE/siRNA complexes ( ).

Article Title: Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients
Article Snippet: RNA quantity was assessed using the Nanodrop One spectrophotometer (Thermo Fischer Scientific). .. Up to 500 ng of total RNA were reverse transcribed using the GoScriptTM Reverse Kit (Promega, San Luis Obispo, CA, USA) and the expression of selected mesenchymal, adipogenic and fibrogenic genes was quantified by real-time quantitative PCR (qRT-PCR) with Syber Green master mix (GoTaq qPCR Master Mix Kit, Promega), and custom sequence-specific oligonucleotide primer pairs (listed in Supplementary Table ). ..

Reverse Transcription:

Article Title: Cigarette smoke-induced gasdermin D activation in bronchoalveolar macrophages and bronchial epithelial cells dependently on NLRP3
Article Snippet: .. Reverse transcription of RNA into cDNA was carried out with Go script reverse kit (Promega). .. RT-PCR was performed with Fast SYBR Green master mix on an ARIA MX (Stratagene MX3005P, Agilent technologies).

Article Title: Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients.
Article Snippet: RNA quantity was assessed using the Nanodrop One spectrophotometer (Thermo Fischer Scientific). .. Up to 500 ng of total RNA were reverse transcribed using the GoScriptTM Reverse Kit (Promega, San Luis Obispo, CA, USA) and the expression of selected mesenchymal, adipogenic and fibrogenic genes was quantified by real-time quantitative PCR (qRT-PCR) with Syber Green master mix (GoTaq qPCR Master Mix Kit, Promega), and custom sequence-specific oligonucleotide primer pairs (listed in Supplementary Table S2). ..

Article Title: Shaping modern human skull through epigenetic, transcriptional and post-transcriptional regulation of the RUNX2 master bone gene.
Article Snippet: Isolated RNA was quantified using a UV spectrophotometer (DU 800 Beckman Coulter, Brea, CA, USA). .. Up to 500 ng/μl of total RNA was reverse transcribed with GoScriptTM Reverse Kit (Promega, San Luis Obispo, CA, USA) following the manufacturer’s protocol. .. Quantitative Real Time PCR (qPCR) using GoTaq(R) qPCR Master Mix (Promega) was performed to evaluate the expression of osteogenic markers at different time points, (OSX and ALP) and of the different RUNX2 transcript variants by means of specific primer pairs22.

Article Title: Targeted allele-specific FGFR2 knockdown via human recombinant ferritin nanoparticles for personalized treatment of Crouzon syndrome
Article Snippet: Isolated RNA was quantified using a UV spectrophotometer (NanoDrop One/OneC Microvolume UV-Vis, Thermo Scientific). .. Then, up to 500 ng/μL of total RNA of each sample were reverse transcribed into cDNA using GoScript Reverse Kit (Promega) following the manufacturer’s protocol. qPCR of above-mentioned genes was performed using GoTaq(R) qPCR Master Mix (Promega) and TaqMan Universal PCR Master Mix (Thermo Fisher Scientific). .. Specifically, sequence-specific fluorescent oligonucleotide Taqman probes and primers (Thermo Fisher Scientific) were designed to evaluate the expression levels of wild-type and mutant FGFR2 transcripts in CS patient cells treated with siRNAs and with HFT-HIS-PASE/siRNA complexes ( ).

Article Title: Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients
Article Snippet: RNA quantity was assessed using the Nanodrop One spectrophotometer (Thermo Fischer Scientific). .. Up to 500 ng of total RNA were reverse transcribed using the GoScriptTM Reverse Kit (Promega, San Luis Obispo, CA, USA) and the expression of selected mesenchymal, adipogenic and fibrogenic genes was quantified by real-time quantitative PCR (qRT-PCR) with Syber Green master mix (GoTaq qPCR Master Mix Kit, Promega), and custom sequence-specific oligonucleotide primer pairs (listed in Supplementary Table ). ..

Expressing:

Article Title: Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients.
Article Snippet: RNA quantity was assessed using the Nanodrop One spectrophotometer (Thermo Fischer Scientific). .. Up to 500 ng of total RNA were reverse transcribed using the GoScriptTM Reverse Kit (Promega, San Luis Obispo, CA, USA) and the expression of selected mesenchymal, adipogenic and fibrogenic genes was quantified by real-time quantitative PCR (qRT-PCR) with Syber Green master mix (GoTaq qPCR Master Mix Kit, Promega), and custom sequence-specific oligonucleotide primer pairs (listed in Supplementary Table S2). ..

Article Title: Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients
Article Snippet: RNA quantity was assessed using the Nanodrop One spectrophotometer (Thermo Fischer Scientific). .. Up to 500 ng of total RNA were reverse transcribed using the GoScriptTM Reverse Kit (Promega, San Luis Obispo, CA, USA) and the expression of selected mesenchymal, adipogenic and fibrogenic genes was quantified by real-time quantitative PCR (qRT-PCR) with Syber Green master mix (GoTaq qPCR Master Mix Kit, Promega), and custom sequence-specific oligonucleotide primer pairs (listed in Supplementary Table ). ..

Sequencing:

Article Title: Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients.
Article Snippet: RNA quantity was assessed using the Nanodrop One spectrophotometer (Thermo Fischer Scientific). .. Up to 500 ng of total RNA were reverse transcribed using the GoScriptTM Reverse Kit (Promega, San Luis Obispo, CA, USA) and the expression of selected mesenchymal, adipogenic and fibrogenic genes was quantified by real-time quantitative PCR (qRT-PCR) with Syber Green master mix (GoTaq qPCR Master Mix Kit, Promega), and custom sequence-specific oligonucleotide primer pairs (listed in Supplementary Table S2). ..

Article Title: Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients
Article Snippet: RNA quantity was assessed using the Nanodrop One spectrophotometer (Thermo Fischer Scientific). .. Up to 500 ng of total RNA were reverse transcribed using the GoScriptTM Reverse Kit (Promega, San Luis Obispo, CA, USA) and the expression of selected mesenchymal, adipogenic and fibrogenic genes was quantified by real-time quantitative PCR (qRT-PCR) with Syber Green master mix (GoTaq qPCR Master Mix Kit, Promega), and custom sequence-specific oligonucleotide primer pairs (listed in Supplementary Table ). ..

Polymerase Chain Reaction:

Article Title: Targeted allele-specific FGFR2 knockdown via human recombinant ferritin nanoparticles for personalized treatment of Crouzon syndrome
Article Snippet: Isolated RNA was quantified using a UV spectrophotometer (NanoDrop One/OneC Microvolume UV-Vis, Thermo Scientific). .. Then, up to 500 ng/μL of total RNA of each sample were reverse transcribed into cDNA using GoScript Reverse Kit (Promega) following the manufacturer’s protocol. qPCR of above-mentioned genes was performed using GoTaq(R) qPCR Master Mix (Promega) and TaqMan Universal PCR Master Mix (Thermo Fisher Scientific). .. Specifically, sequence-specific fluorescent oligonucleotide Taqman probes and primers (Thermo Fisher Scientific) were designed to evaluate the expression levels of wild-type and mutant FGFR2 transcripts in CS patient cells treated with siRNAs and with HFT-HIS-PASE/siRNA complexes ( ).



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