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histone h2b coding sequence  (Addgene inc)


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    Structured Review

    Addgene inc histone h2b coding sequence
    Histone H2b Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+coding+sequence/H2B-GFP+(Plasmid+%2311680)/pm41895260-276-1-8
    Average 95 stars, based on 197 article reviews
    histone h2b coding sequence - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Modular engineering of thermoresponsive allosteric proteins.
    Article Snippet: The Renilla luciferase expression plasmid was purchased from Promega. .. H2B–GFP was a gift from G. Wahl (Addgene, plasmid 11680). pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA was a gift from F. Zhang (Addgene, plasmid 61591) and sgRNA1_Tet-inducible luciferase reporter and Tet-inducible mCherry reporter were gifts from M. Sato (Addgene, plasmids 64161 and 64128). ..

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Article Title: Distinct cMET inhibitors uncover pharmacological heterogeneity in SHH medulloblastoma cell lines
    Article Snippet: .. H2B-GFP plasmid harbouring the neomycin resistance gene (Addgene, #11680) was purified by using Qiagen Plasmid Midi Kit and transfected in DAOY and ONS76 cells (2 μg) plated in 6 well plates by using Lipofectamin 2000 in a ratio 1:3 of DNA to lipofectamin. .. Transfected DAOY and ONS76 cells were kept in media enriched with neomycin (Roche) at 1 mg/mL concentration for 2 weeks and the presence of GFP-positive cells was confirmed by microscopy before performing further analyses.

    Article Title: Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.
    Article Snippet: The following cDNAs were synthesized with optimized codons by GeneArt (Thermo Fisher Scientific, Waltham, MA): sfGFP1-10, 3 CFP21-10, 39 mNeonGreen3A1-10, 11 sfCherry3Csp1-10, mRuby41-10, 10 GB1,18 ΔSUMOstar.19 Additional split fluorescent protein variants were generated through site-directed mutagenesis of existing templates: Cerulean1-10 10 and YFP11-10 39 from sfGFP1-10, mNeonGreen3K1-10 11 from mNeonGreen3A1-10, and sfCherry3C1-10 12 from sfCherry21-10 (Addgene: #82603). .. The Histone H2B coding sequence was subcloned from Addgene (plasmid # 11680 for Histone H2B; Cambridge, MA). .. Vector construction for stable cell line generation A base Tol2 transposon vector (pT2A-IRES-iRFP670) was constructed by subcloning iRFP670 cDNA40 with an internal ribosome entry site (IRES)41 into the pT2A vector.37 CFP21-10 cDNA was subsequently PCR-amplified and inserted into this base vector using InFusion assembly (Clontech, Mountain View, CA) to generate pT2A_CFP21-10-IRES-iRFP670.

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines.
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Luciferase:

    Article Title: Modular engineering of thermoresponsive allosteric proteins.
    Article Snippet: The Renilla luciferase expression plasmid was purchased from Promega. .. H2B–GFP was a gift from G. Wahl (Addgene, plasmid 11680). pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA was a gift from F. Zhang (Addgene, plasmid 61591) and sgRNA1_Tet-inducible luciferase reporter and Tet-inducible mCherry reporter were gifts from M. Sato (Addgene, plasmids 64161 and 64128). ..

    Transfection:

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Article Title: Distinct cMET inhibitors uncover pharmacological heterogeneity in SHH medulloblastoma cell lines
    Article Snippet: .. H2B-GFP plasmid harbouring the neomycin resistance gene (Addgene, #11680) was purified by using Qiagen Plasmid Midi Kit and transfected in DAOY and ONS76 cells (2 μg) plated in 6 well plates by using Lipofectamin 2000 in a ratio 1:3 of DNA to lipofectamin. .. Transfected DAOY and ONS76 cells were kept in media enriched with neomycin (Roche) at 1 mg/mL concentration for 2 weeks and the presence of GFP-positive cells was confirmed by microscopy before performing further analyses.

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines.
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Stable Transfection:

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines.
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Expressing:

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Article Title: Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines.
    Article Snippet: .. Both cell types were transfected with a plasmid encoding the H2B-GFP fusion protein from Geoff Wahl (Addgene plasmid #11680) to generate a stable cell line constitutively expressing human histone H2B, one of the core proteins in DNA packaging, tagged with Green Fluorescent Protein (GFP). ..

    Purification:

    Article Title: Distinct cMET inhibitors uncover pharmacological heterogeneity in SHH medulloblastoma cell lines
    Article Snippet: .. H2B-GFP plasmid harbouring the neomycin resistance gene (Addgene, #11680) was purified by using Qiagen Plasmid Midi Kit and transfected in DAOY and ONS76 cells (2 μg) plated in 6 well plates by using Lipofectamin 2000 in a ratio 1:3 of DNA to lipofectamin. .. Transfected DAOY and ONS76 cells were kept in media enriched with neomycin (Roche) at 1 mg/mL concentration for 2 weeks and the presence of GFP-positive cells was confirmed by microscopy before performing further analyses.

    Sequencing:

    Article Title: Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.
    Article Snippet: The following cDNAs were synthesized with optimized codons by GeneArt (Thermo Fisher Scientific, Waltham, MA): sfGFP1-10, 3 CFP21-10, 39 mNeonGreen3A1-10, 11 sfCherry3Csp1-10, mRuby41-10, 10 GB1,18 ΔSUMOstar.19 Additional split fluorescent protein variants were generated through site-directed mutagenesis of existing templates: Cerulean1-10 10 and YFP11-10 39 from sfGFP1-10, mNeonGreen3K1-10 11 from mNeonGreen3A1-10, and sfCherry3C1-10 12 from sfCherry21-10 (Addgene: #82603). .. The Histone H2B coding sequence was subcloned from Addgene (plasmid # 11680 for Histone H2B; Cambridge, MA). .. Vector construction for stable cell line generation A base Tol2 transposon vector (pT2A-IRES-iRFP670) was constructed by subcloning iRFP670 cDNA40 with an internal ribosome entry site (IRES)41 into the pT2A vector.37 CFP21-10 cDNA was subsequently PCR-amplified and inserted into this base vector using InFusion assembly (Clontech, Mountain View, CA) to generate pT2A_CFP21-10-IRES-iRFP670.



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