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opal 480  (Quanterix)


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    Structured Review

    Quanterix opal 480
    Opal 480, supplied by Quanterix, used in various techniques. Bioz Stars score: 99/100, based on 1157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fp1500001kt/Opal+480/custom%40fp1500001kt%4042012981
    Average 99 stars, based on 1157 article reviews
    opal 480 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Incubation:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Chloramphenicol Acetyltransferase Assay:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Modification:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Amplification:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Immunohistochemistry:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Fluorescence:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Microscopy:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Transcranial Magnetic Stimulation Electroencephalography:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Imaging:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Staining:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    RNAscope:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til

    Multiplex Assay:

    Article Title: The thymus gland is a target and maintainer of amyotrophic lateral sclerosis pathophysiology
    Article Snippet: peroxide dismutase 1 (SOD1) mRNA specific hybridization, the probe RNAscope™ Probe - Hs-SOD1-No-XMm-C1 (ACD Bio; Catalog Number 1147991-C1) was used and detection was possible using the Opal™ 570 dye (Akoya Biosciences; Catalog Number FP1488001KT). At the end of the protocol, slides were mounted using ProLong™ Gold Antifade mounting medium (Thermo Fisher Scientific), the images were obtained as til



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    Image Search Results


    Example images of immunofluorescent staining on a pancreatic cancer core sample from a tissue microarray from different antibodies showing pan-cytokeratin (red, Opal 570, top left), SMAD4 (green, Opal 620, top right), and an unnamed biomarker (yellow, Opal 520, bottom left). The merged images are shown in the bottom right. Nuclei were stained blue with DAPI. Total cellular fluorescence intensity units of SMAD4 per cell in the pan-cytokeratin positive cells were determined by a Vectra Polaris microscope and Inform software.

    Journal: International journal of radiation oncology, biology, physics

    Article Title: High SMAD4 Expression is Associated with Improved Clinical Outcomes in Patients with Resectable Pancreatic Cancer: An Analysis of NRG Oncology/RTOG 9704

    doi: 10.1016/j.ijrobp.2026.09.014

    Figure Lengend Snippet: Example images of immunofluorescent staining on a pancreatic cancer core sample from a tissue microarray from different antibodies showing pan-cytokeratin (red, Opal 570, top left), SMAD4 (green, Opal 620, top right), and an unnamed biomarker (yellow, Opal 520, bottom left). The merged images are shown in the bottom right. Nuclei were stained blue with DAPI. Total cellular fluorescence intensity units of SMAD4 per cell in the pan-cytokeratin positive cells were determined by a Vectra Polaris microscope and Inform software.

    Article Snippet: Slides were counterstained with Spectral DAPI (Akoya Biosciences Cat. # FP1490) at a 1:100 dilution for 8 minutes.

    Techniques: Staining, Tissue, Microarray, Biomarker Discovery, Fluorescence, Microscopy, Software