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coronavirus pan monoclonal antibody fipv3-70  (Thermo Fisher)


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    Thermo Fisher coronavirus pan monoclonal antibody fipv3-70
    Coronavirus Pan Monoclonal Antibody Fipv3 70, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/monoclonal+mouse+anti+coronavirus+antibody+fipv3+70/pmc11960445-162-5-10
    Average 90 stars, based on 1 article reviews
    coronavirus pan monoclonal antibody fipv3-70 - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Structural-based virtual screening and in vitro assays for small molecules inhibiting the feline coronavirus 3CL protease as a surrogate platform for coronaviruses
    Article Snippet: The cells were incubated with primary antibody (mouse anti-FIPV3-70, dilution 1:500, ThermoFisher, Carlsbad, USA) at 37 °C for 1 h. After a rinse with PBS, cells were incubated with secondary antibody (goat anti-mouse IgG-HRP, dilution 1:400, Kirkegaard & Perry Laboratories, Madison, USA) at 37 °C for 1 h, and antigen-antibody reaction was visualized after adding DAB substrate (DAKO, Santa Clara, USA).

    Article Title: Application of versatile reverse genetics system for feline coronavirus
    Article Snippet: The cells were stained with Coronavirus pan Monoclonal Antibody FIPV3-70 (Thermo Fisher Scientific) and Goat anti-Mouse IgG (H+L) Alexa Fluor 488 (Thermo Fisher Scientific) and then mounted with 4 ́,6-diamidino-2-phenylindole (Dojindo).

    Article Title: Time-dependent viral interference between influenza virus and coronavirus in the infection of differentiated porcine airway epithelial cells
    Article Snippet: The samples were blocked with 1% BSA, then incubated with a monoclonal mouse anti-coronavirus-antibody (FIPV3-70; 1:1000, Thermo Fischer, Scientific) followed by Alexa Fluor® 488 conjugated secondary antibody staining (Thermo Fisher).

    Article Title: The Cell Tropism of Porcine Respiratory Coronavirus for Airway Epithelial Cells Is Determined by the Expression of Porcine Aminopeptidase N
    Article Snippet: The coronavirus nucleocapsid protein was stained by a monoclonal mouse anti-coronavirus-antibody (FIPV3-70; 1:1000, Invitrogen, Carlsbad, CA, USA), and followed by incubation with fluorescent secondary antibody (1: 1,000; Alexa Fluor 488 anti-mouse IgG [H + L] antibody, Thermo Fisher, Massachusetts, USA).

    Article Title: First study on in vitro antiviral and virucidal effects of flavonoids against feline infectious peritonitis virus at the early stage of infection
    Article Snippet: The fixed cells were incubated with primary antibody (mouse anti-FIPV3-70, dilution 1:500, ThermoFisher, Carlsbad, USA) at 37°C in a moist chamber for 1 h and washed with PBST before incubation with goat anti-mouse IgG-HRP antibody (dilution 1:800, ThermoFisher), at 37°C in a moist chamber for 1 h. The FIPV antigens were visualized by adding the DAB substrate (DAKO, Santa Clara, USA), the brown color of which was developed in the cytoplasm of the infected cells.

    Article Title: First versatile reverse genetics system for feline coronavirus
    Article Snippet: The cells were stained with Coronavirus pan Monoclonal Antibody FIPV3-70 (Thermo Scientific) and Goat anti-Mouse IgG (H+L) Alexa Fluor 488 (Thermo Scientific) and then mounted with 4’,6-diamidino-2-phenylindole (DAPI; Dojindo).

    Incubation:

    Article Title: In silico and in vitro analysis of small molecules and natural compounds targeting the 3CL protease of feline infectious peritonitis virus
    Article Snippet: .. The primary antibody for detection of FIPV, a mouse monoclonal antibody specific to pan-coronavirus FIPV3–70 (dilution 1:500, ThermoFisher, Carlsbad, USA), was incubated with the infected cells at 37 °C for 1 h. These treated cells were then washed using PBST, following which they were incubated with the secondary antibody, a goat anti-mouse IgG-HRP antibody (dilution 1:400, Kirkegaard & Perry Laboratories, Madison, USA), at 37 °C for 1 h. The antigen-antibody reaction was stained using DAB substrate (DAKO, Santa Clara, USA) and observed under a phase-contrast inverted microscope (Olympus CKX41, Tokyo, Japan). ..

    Infection:

    Article Title: In silico and in vitro analysis of small molecules and natural compounds targeting the 3CL protease of feline infectious peritonitis virus
    Article Snippet: .. The primary antibody for detection of FIPV, a mouse monoclonal antibody specific to pan-coronavirus FIPV3–70 (dilution 1:500, ThermoFisher, Carlsbad, USA), was incubated with the infected cells at 37 °C for 1 h. These treated cells were then washed using PBST, following which they were incubated with the secondary antibody, a goat anti-mouse IgG-HRP antibody (dilution 1:400, Kirkegaard & Perry Laboratories, Madison, USA), at 37 °C for 1 h. The antigen-antibody reaction was stained using DAB substrate (DAKO, Santa Clara, USA) and observed under a phase-contrast inverted microscope (Olympus CKX41, Tokyo, Japan). ..

    Staining:

    Article Title: In silico and in vitro analysis of small molecules and natural compounds targeting the 3CL protease of feline infectious peritonitis virus
    Article Snippet: .. The primary antibody for detection of FIPV, a mouse monoclonal antibody specific to pan-coronavirus FIPV3–70 (dilution 1:500, ThermoFisher, Carlsbad, USA), was incubated with the infected cells at 37 °C for 1 h. These treated cells were then washed using PBST, following which they were incubated with the secondary antibody, a goat anti-mouse IgG-HRP antibody (dilution 1:400, Kirkegaard & Perry Laboratories, Madison, USA), at 37 °C for 1 h. The antigen-antibody reaction was stained using DAB substrate (DAKO, Santa Clara, USA) and observed under a phase-contrast inverted microscope (Olympus CKX41, Tokyo, Japan). ..

    Inverted Microscopy:

    Article Title: In silico and in vitro analysis of small molecules and natural compounds targeting the 3CL protease of feline infectious peritonitis virus
    Article Snippet: .. The primary antibody for detection of FIPV, a mouse monoclonal antibody specific to pan-coronavirus FIPV3–70 (dilution 1:500, ThermoFisher, Carlsbad, USA), was incubated with the infected cells at 37 °C for 1 h. These treated cells were then washed using PBST, following which they were incubated with the secondary antibody, a goat anti-mouse IgG-HRP antibody (dilution 1:400, Kirkegaard & Perry Laboratories, Madison, USA), at 37 °C for 1 h. The antigen-antibody reaction was stained using DAB substrate (DAKO, Santa Clara, USA) and observed under a phase-contrast inverted microscope (Olympus CKX41, Tokyo, Japan). ..



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    The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

    doi: 10.1038/s41598-024-80708-4

    Figure Lengend Snippet: The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The primary antibodies were used to detect the expression levels of the BCoV-nucleocapsid mouse anti-bovine monoclonal (clone: FIPV3-70; Cat. No. MA1-82,189), BCoV-spike rabbit anti-bovine polyclonal (cat. no. PA5-117562), and β-actin rabbit anti-bovine polyclonal (Catalogue number: PA1-46296) antibodies were purchased from Invitrogen.

    Techniques: Over Expression, Infection, In Silico, Binding Assay, Sequencing, Quantitative RT-PCR, Transfection, Viral Plaque Assay, Western Blot, Expressing, Comparison