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bcov-nucleocapsid mouse anti-bovine monoclonal (clone: fipv3-70; cat. no. ma1-82189)  (Thermo Fisher)


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    Thermo Fisher bcov-nucleocapsid mouse anti-bovine monoclonal (clone: fipv3-70; cat. no. ma1-82189)
    Bcov Nucleocapsid Mouse Anti Bovine Monoclonal (Clone: Fipv3 70; Cat. No. Ma1 82189), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/pm39490206-107-8-20
    Average 90 stars, based on 1 article reviews
    bcov-nucleocapsid mouse anti-bovine monoclonal (clone: fipv3-70; cat. no. ma1-82189) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Blocking Assay:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Isolation:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Construct:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Software:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Control:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Polyacrylamide Gel Electrophoresis:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    SDS Page:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Infection:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Marker:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Western Blot:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Expressing:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Sequencing:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Membrane:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Binding Assay:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    RNA Binding Assay:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Reverse Transcription:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Polymerase Chain Reaction:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Quantitative RT-PCR:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.

    Ex Vivo:

    Article Title: The ex vivo infection model of the peripheral bovine mononuclear cells (PBMCs) and the bovine spleen cells with the bovine coronavirus (BCoV) induced a differential expression of the host cytokine genes profiles and modulates the virus replication.
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% BSA (1:100) overnight at 4oC in the dark.

    Article Title: Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein
    Article Snippet: The cell supernatants were removed, and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark.

    Article Title: The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication
    Article Snippet: Without washing, the cells were incubated in primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Clone: FIPV3-70; Cat. No. MA1-82189) purchased from Invitrogen diluted in 0.5% B.S.A. (1:100) overnight at 4°C in the dark.



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    Image Search Results


    The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

    doi: 10.1038/s41598-024-80708-4

    Figure Lengend Snippet: The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The primary antibodies were used to detect the expression levels of the BCoV-nucleocapsid mouse anti-bovine monoclonal (clone: FIPV3-70; Cat. No. MA1-82,189), BCoV-spike rabbit anti-bovine polyclonal (cat. no. PA5-117562), and β-actin rabbit anti-bovine polyclonal (Catalogue number: PA1-46296) antibodies were purchased from Invitrogen.

    Techniques: Over Expression, Infection, In Silico, Binding Assay, Sequencing, Quantitative RT-PCR, Transfection, Viral Plaque Assay, Western Blot, Expressing, Comparison