Review



anti 229e n  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    Novus Biologicals anti 229e n
    Anti 229e N, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/Coronavirus+Antibody+(FIPV3-70)+-+BSA+Free/pmc11543989-488-4-7
    Average 92 stars, based on 5 article reviews
    anti 229e n - by Bioz Stars, 2026-10
    92/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals
    Article Snippet: Adenovirus E1A and hexon proteins were detected using rabbit α-E1A (Santa Cruz sc-430) and undiluted hybridoma (2Hx-2) media, respectively, as previously detailed [ ]. .. To measure the effect of the compound on HCoV-229E, HCoV-OC43, or SARS-CoV-2 nucleocapsid (N) protein expression, mouse α-coronavirus antibody clone FIPV3-70 (Novus Biologicals, Cat: NB10064754), mouse α-coronavirus group antigen antibody clone 542-7D (Millipore Sigma, Cat: MAB9013), or rabbit anti-SARS-CoV-2 nucleocapsid antibody (SinoBiological, Cat: 40143-R019) were used, respectively. .. Changes in HCoV-OC43 spike protein expression were assessed using mouse monoclonal antibody 541-8F (Sigma-Aldrich, MAB9012).

    Article Title: Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals
    Article Snippet: Adenovirus E1A and hexon proteins were detected using rabbit α-E1A (Santa Cruz sc-430) and undiluted hybridoma (2Hx-2) media, respectively, as previously detailed . .. To measure the effect of compound on HCoV-229E, HCoV-OC43, or SARS CoV-2 nucleocapsid (N) protein expression, mouse α-coronavirus antibody clone FIPV3-70 (Novus Biologicals, Cat: NB10064754), mouse α-coronavirus group antigen antibody clone 542-7D (Millipore Sigma, Cat: MAB9013), or rabbit anti-SARS CoV2 nucleocapsid antibody (SinoBiological, Cat: 40143-R019) were used, respectively. .. Changes in HCoV-OC43 spike protein expression were assessed using mouse monoclonal antibody 541-8F (Sigma-Aldrich, MAB9012).

    other:

    Article Title: On a path toward a broad-spectrum anti-viral: inhibition of HIV-1 and coronavirus replication by SR kinase inhibitor harmine
    Article Snippet: Type Size (kDa) Dilution Company Catalog No. Gag p24 Ms 55, 41, 24 1:500 in 1X TBST NIH AIDS Reagent #1513 Env gp41 Hu 160 1:1000 in 1X TBST NIH AIDS Reagent #531 Tat 1D9 Ms 16, 14 1:300 in 1X TBST NIH AIDS Reagent #7383 DYRK1A Rb 90 1:1000 in 1 X TBST Cell Signaling Technologies #8765S GAPDH Rb 35.8 1:3000 in 1X TBST Sigma-Aldrich G9545 Tubulin Rb 55 1:3000 in 1 X TBST Cell Signaling Technologies #2144S CLK1 Ms 60 1:1000 in 5% BSA-TBST Santa Cruz #515897 CLK2 Rb 60 1:1000 in 5% BSA-TBST Abcam ab65082 CLK3 Rb 60 1:1000 in 5% BSA-TBST Abnova H00001198-M05 SRPK1 Rb 110 1:2000 in 5% BSA-TBST Cedarlane OAAN01583 SRSF1 (ASF/SF2) Ms 32 1:1000 in 1X TBST Life Technologies 32-4500 SRSF2 (SC35) Rb ~35 1:1000 in 1X TBST Abcam ab11826 SRSF3 (SRp20) Ms 20 1:1000 in 1X TBST Life Technologies 33-4200 SRSF4 (SRp75) Rb 75 1:2000 in 1X TBST Novus Biologicals NBP2-04144 SRSF5 Rb ~40 1:1000 in 1X TBST MBL RN082PW SRSF6 (SRp55) Rb 55 1:2000 in 1X TBST Novus Biologicals NBP2-04142 SRSF7 (9G8) Rb ~35 1:2000 in 1X TBST Abcam ab137247 SRSF9 (SRp30c) Rb 27 1:1000 in 1X TBST MBL RN081PW SRSF10 (FUSIP1) Ms 37 1:1000 in 1X TBST Novus Biologicals H00010772-M07 Tra2ß Rb ~40 1:1000 in 1X TBST Abcam ab31353 Feline anti-coronavirus antibody (FIPV3-70 for HCoV-229E N) Ms ~60 1:4000 in 1 X TBST Novus Biologicals NB10064754

    Staining:

    Article Title: Targeting protein homeostasis with small molecules as a strategy for the development of pan-coronavirus antiviral therapies
    Article Snippet: .. Blots were stained with anti-229E N (1:5000, Novus Biologicals NB10064754), Sars-CoV-2 N (1:1000, ThermoFisher Scientific MA5-29981), AMPKα [1:500, Cell Signaling Technology (CST) 2532S], Phospho-AMPKα (Thr172) (1:500, CST 2535S), mTOR (1:1000, CST 2983S), Phospho-mTOR (Ser2448) (1:500, CST 5536S), Akt (pan) (1:2000, CST 4691S), Phospho-Akt (Ser473) (1:1000, CST 4060S), and GAPDH (1:20000, Abcam ab8245) for loading control. ..

    Control:

    Article Title: Targeting protein homeostasis with small molecules as a strategy for the development of pan-coronavirus antiviral therapies
    Article Snippet: .. Blots were stained with anti-229E N (1:5000, Novus Biologicals NB10064754), Sars-CoV-2 N (1:1000, ThermoFisher Scientific MA5-29981), AMPKα [1:500, Cell Signaling Technology (CST) 2532S], Phospho-AMPKα (Thr172) (1:500, CST 2535S), mTOR (1:1000, CST 2983S), Phospho-mTOR (Ser2448) (1:500, CST 5536S), Akt (pan) (1:2000, CST 4691S), Phospho-Akt (Ser473) (1:1000, CST 4060S), and GAPDH (1:20000, Abcam ab8245) for loading control. ..



    Similar Products

    coronavirus pan monoclonal antibody fipv3-70  (Thermo Fisher)
    90
    Thermo Fisher coronavirus pan monoclonal antibody fipv3-70
    Coronavirus Pan Monoclonal Antibody Fipv3 70, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/monoclonal+mouse+anti+coronavirus+antibody+fipv3+70/pmc11960445-162-5-10
    Average 90 stars, based on 1 article reviews
    coronavirus pan monoclonal antibody fipv3-70 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    mouse α-coronavirus antibody clone fipv3-70  (Novus Biologicals)
    90
    Novus Biologicals mouse α-coronavirus antibody clone fipv3-70
    Mouse α Coronavirus Antibody Clone Fipv3 70, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/mouse+%CE%B1+coronavirus+antibody+clone+fipv3+70/pm39861843-77-20-21
    Average 90 stars, based on 1 article reviews
    mouse α-coronavirus antibody clone fipv3-70 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    mouse α coronavirus antibody  (Novus Biologicals)
    92
    Novus Biologicals mouse α coronavirus antibody
    Mouse α Coronavirus Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/Coronavirus+Antibody+(FIPV3-70)+%5BAllophycocyanin%5D/pmc11768839-94-16-21
    Average 92 stars, based on 1 article reviews
    mouse α coronavirus antibody - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier
    fipv3 70  (Novus Biologicals)
    92
    Novus Biologicals fipv3 70
    Fipv3 70, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/Coronavirus+Antibody+(FIPV3-70)+-+BSA+Free/pmc11768839-94-20-21
    Average 92 stars, based on 1 article reviews
    fipv3 70 - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier
    anti-coronavirus nucleoprotein, clone fipv3-70  (Custom Monoclonals International)
    90
    Custom Monoclonals International anti-coronavirus nucleoprotein, clone fipv3-70
    Anti Coronavirus Nucleoprotein, Clone Fipv3 70, supplied by Custom Monoclonals International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/monoclonal+antibody+fcv3+70/10__3390_slash_biology13120997-106-0-23
    Average 90 stars, based on 1 article reviews
    anti-coronavirus nucleoprotein, clone fipv3-70 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    bcov-nucleocapsid mouse anti-bovine monoclonal (clone: fipv3-70; cat. no. ma1-82,189)  (Thermo Fisher)
    90
    Thermo Fisher bcov-nucleocapsid mouse anti-bovine monoclonal (clone: fipv3-70; cat. no. ma1-82,189)
    The overexpression of the bovine miRNA16a inhibited <t>BCoV</t> replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of <t>BCoV-nucleocapsid</t> (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Bcov Nucleocapsid Mouse Anti Bovine Monoclonal (Clone: Fipv3 70; Cat. No. Ma1 82,189), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/pmc11599744-130-12-40
    Average 90 stars, based on 1 article reviews
    bcov-nucleocapsid mouse anti-bovine monoclonal (clone: fipv3-70; cat. no. ma1-82,189) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    anti 229e n  (Novus Biologicals)
    92
    Novus Biologicals anti 229e n
    The overexpression of the bovine miRNA16a inhibited <t>BCoV</t> replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of <t>BCoV-nucleocapsid</t> (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Anti 229e N, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/Coronavirus+Antibody+(FIPV3-70)+-+BSA+Free/pmc11543989-488-4-7
    Average 92 stars, based on 1 article reviews
    anti 229e n - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier
    bcov-nucleocapsid mouse anti-bovine monoclonal (clone: fipv3-70; cat. no. ma1-82189)  (Thermo Fisher)
    90
    Thermo Fisher bcov-nucleocapsid mouse anti-bovine monoclonal (clone: fipv3-70; cat. no. ma1-82189)
    The overexpression of the bovine miRNA16a inhibited <t>BCoV</t> replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of <t>BCoV-nucleocapsid</t> (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Bcov Nucleocapsid Mouse Anti Bovine Monoclonal (Clone: Fipv3 70; Cat. No. Ma1 82189), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/pm39490206-107-8-20
    Average 90 stars, based on 1 article reviews
    bcov-nucleocapsid mouse anti-bovine monoclonal (clone: fipv3-70; cat. no. ma1-82189) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

    doi: 10.1038/s41598-024-80708-4

    Figure Lengend Snippet: The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The primary antibodies were used to detect the expression levels of the BCoV-nucleocapsid mouse anti-bovine monoclonal (clone: FIPV3-70; Cat. No. MA1-82,189), BCoV-spike rabbit anti-bovine polyclonal (cat. no. PA5-117562), and β-actin rabbit anti-bovine polyclonal (Catalogue number: PA1-46296) antibodies were purchased from Invitrogen.

    Techniques: Over Expression, Infection, In Silico, Binding Assay, Sequencing, Quantitative RT-PCR, Transfection, Viral Plaque Assay, Western Blot, Expressing, Comparison