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anti-coronavirus nucleoprotein, clone fipv3-70  (Custom Monoclonals International)

 
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    Custom Monoclonals International anti-coronavirus nucleoprotein, clone fipv3-70
    Anti Coronavirus Nucleoprotein, Clone Fipv3 70, supplied by Custom Monoclonals International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/monoclonal+antibody+fcv3+70/10__3390_slash_biology13120997-106-0-23
    Average 90 stars, based on 1 article reviews
    anti-coronavirus nucleoprotein, clone fipv3-70 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Efficacy of Oral Remdesivir Compared to GS-441524 for Treatment of Cats with Naturally Occurring Effusive Feline Infectious Peritonitis: A Blinded, Non-Inferiority Study.
    Article Snippet: .. Tissues with pyogranulomatous perivascular inflammation, characteristic of FIP, were further evaluated by an immunohistochemistry (IHC) assay to detect coronaviral antigen (FIPV3-70, Custom Monoclonals International, Sacramento, CA, USA) [36]. ..

    Article Title: The Causes of Canine Myocarditis and Myocardial Fibrosis Are Elusive by Targeted Molecular Testing: Retrospective Analysis and Literature Review
    Article Snippet: .. Immunohistochemistry and In Situ Hybridization Immunohistochemistry (IHC) for CDV (mouse monoclonal gift from James A. Baker Institute of Cornell University, Ithaca, NY), CPV-1 (rabbit polyclonal gift from James A. Baker Institute Carmichael Laboratory Cornell University), adenovirus (MAB8052; Millipore, Temecula, CA), WNV (clone 7H2; VRL, Gaithersburg, MD), and coronavirus (FIPV3-70; Custom Monoclonals International Sacramento, CA), was performed on a subset of cases by the AHDC Histopathology Laboratory. ..

    Article Title: Serologic, Virologic and Pathologic Features of Cats with Naturally Occurring Feline Infectious Peritonitis Enrolled in Antiviral Clinical Trials
    Article Snippet: .. Tissues with pyogranulomatous perivascular inflammation, characteristic of FIP, were further evaluated by an immunohistochemistry (IHC) assay to detect the coronaviral antigen (FIPV3-70, Custom Monoclonals International, Sacramento, CA, USA) [ ]. ..

    Article Title: Open label clinical trial of orally administered molnupiravir as a first‐line treatment for naturally occurring effusive feline infectious peritonitis
    Article Snippet: .. Immunohistochemistry (IHC) to detect FCoV antigen (FIPV3‐70, Custom Monoclonals International, Sacramento, CA) was performed as previously described. ..

    Article Title: Open label clinical trial of orally administered molnupiravir as a first-line treatment for naturally occurring effusive feline infectious peritonitis.
    Article Snippet: .. Immunohistochemistry (IHC) to detect FCoV antigen (FIPV3-70, Custom Monoclonals International, Sacramento, CA) was performed as previously described.20 Interpretation of histopathology and IHC was performed by a single veterinary anatomic pathologist (B.G. ..

    other:

    Article Title: Fatal Feline Leukemia Virus-Associated Enteritis in a Wild Eurasian Lynx (Lynx lynx) in Germany
    Article Snippet: Immunohistochemistry of the small and large intestines and mesenteric lymph nodes was performed for the detection of FeLV (anti-gp85/70 envelope protein, clone C11D8, Custom Monoclonals International Corp., Sacramento, CA, USA, mouse, monoclonal, 1:200), feline panleukopenia virus (FPV)/canine parvovirus (CPV; anti-capsid immunodominant region #1, clone CPV1-2A1, Custom Monoclonals International Corp., Sacramento, CA, USA, mouse, monoclonal, 1:500), and feline coronavirus (FCoV; anti-coronavirus nucleoprotein; clone FIPV3-70, Custom Monoclonals International Corp., Sacramento, CA, USA, mouse, monoclonal, 1:10,000) using the avidin–biotin peroxidase complex (#PK 6100, Vectastain elite ABC kit, Vector Laboratories, Burlingame, CA, USA).

    Article Title: Serologic, Virologic and Pathologic Features of Cats with Naturally Occurring Feline Infectious Peritonitis Enrolled in Antiviral Clinical Trials
    Article Snippet: Positive control FIPV3-70 and CCV monoclonal antibodies were added to two strips at 1:500 (Custom Monoclonals International, Sacramento, CA, USA).

    In Situ Hybridization:

    Article Title: The Causes of Canine Myocarditis and Myocardial Fibrosis Are Elusive by Targeted Molecular Testing: Retrospective Analysis and Literature Review
    Article Snippet: .. Immunohistochemistry and In Situ Hybridization Immunohistochemistry (IHC) for CDV (mouse monoclonal gift from James A. Baker Institute of Cornell University, Ithaca, NY), CPV-1 (rabbit polyclonal gift from James A. Baker Institute Carmichael Laboratory Cornell University), adenovirus (MAB8052; Millipore, Temecula, CA), WNV (clone 7H2; VRL, Gaithersburg, MD), and coronavirus (FIPV3-70; Custom Monoclonals International Sacramento, CA), was performed on a subset of cases by the AHDC Histopathology Laboratory. ..

    Histopathology:

    Article Title: The Causes of Canine Myocarditis and Myocardial Fibrosis Are Elusive by Targeted Molecular Testing: Retrospective Analysis and Literature Review
    Article Snippet: .. Immunohistochemistry and In Situ Hybridization Immunohistochemistry (IHC) for CDV (mouse monoclonal gift from James A. Baker Institute of Cornell University, Ithaca, NY), CPV-1 (rabbit polyclonal gift from James A. Baker Institute Carmichael Laboratory Cornell University), adenovirus (MAB8052; Millipore, Temecula, CA), WNV (clone 7H2; VRL, Gaithersburg, MD), and coronavirus (FIPV3-70; Custom Monoclonals International Sacramento, CA), was performed on a subset of cases by the AHDC Histopathology Laboratory. ..

    Article Title: Open label clinical trial of orally administered molnupiravir as a first-line treatment for naturally occurring effusive feline infectious peritonitis.
    Article Snippet: .. Immunohistochemistry (IHC) to detect FCoV antigen (FIPV3-70, Custom Monoclonals International, Sacramento, CA) was performed as previously described.20 Interpretation of histopathology and IHC was performed by a single veterinary anatomic pathologist (B.G. ..

    Incubation:

    Article Title: Characterization of young and aged ferrets as animal models for SARS-CoV-2 infection with focus on neutrophil extracellular traps
    Article Snippet: .. Subsequently, sections were incubated with mouse monoclonal antibodies directed against SARS-CoV-2 nucleocapsid protein (40143-MM05; Sino Biological Europe; 1:3,200 in PBS with 1% BSA and 0.3% TritonX-100) or FRSCV (FIPV3-70; Custom Monoclonals International; 1:10,000 in PBS with 1% BSA) for 1 h at room temperature. ..

    Bioprocessing:

    Article Title: Characterization of young and aged ferrets as animal models for SARS-CoV-2 infection with focus on neutrophil extracellular traps
    Article Snippet: .. Subsequently, sections were incubated with mouse monoclonal antibodies directed against SARS-CoV-2 nucleocapsid protein (40143-MM05; Sino Biological Europe; 1:3,200 in PBS with 1% BSA and 0.3% TritonX-100) or FRSCV (FIPV3-70; Custom Monoclonals International; 1:10,000 in PBS with 1% BSA) for 1 h at room temperature. ..



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    The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

    doi: 10.1038/s41598-024-80708-4

    Figure Lengend Snippet: The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The primary antibodies were used to detect the expression levels of the BCoV-nucleocapsid mouse anti-bovine monoclonal (clone: FIPV3-70; Cat. No. MA1-82,189), BCoV-spike rabbit anti-bovine polyclonal (cat. no. PA5-117562), and β-actin rabbit anti-bovine polyclonal (Catalogue number: PA1-46296) antibodies were purchased from Invitrogen.

    Techniques: Over Expression, Infection, In Silico, Binding Assay, Sequencing, Quantitative RT-PCR, Transfection, Viral Plaque Assay, Western Blot, Expressing, Comparison