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anti-fcov mouse monoclonal igg2a fipv3-70  (Linaris GmbH)

 
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    Linaris GmbH anti-fcov mouse monoclonal igg2a fipv3-70
    Anti Fcov Mouse Monoclonal Igg2a Fipv3 70, supplied by Linaris GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fipv3+70/fipv3+70+monoclonal+antibody/10__1177_slash_1098612x16630357-57-1-6
    Average 90 stars, based on 1 article reviews
    anti-fcov mouse monoclonal igg2a fipv3-70 - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Diagnostic Value of Detecting Feline Coronavirus RNA and Spike Gene Mutations in Cerebrospinal Fluid to Confirm Feline Infectious Peritonitis
    Article Snippet: A monoclonal anti-FCoV-antibody (clone FIPV3-70, LINARIS GmbH, Dossenheim, Germany) was used as primary antibody and diaminobenzidine-tetrahydrochloride as chromogen.

    Article Title: Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis
    Article Snippet: IHC was performed using clone FIPV3-70 antibody (Linaris Medizinische Produkte GmbH) on formalin-fixed, paraffin-embedded tissue sections.30 For signal detection, the streptavidin–biotin complex method was implemented (VECTASTAIN ABC Kit; Vector Laboratories).

    Article Title: Detection of feline coronavirus mutations in paraffin-embedded tissues in cats with feline infectious peritonitis and controls
    Article Snippet: 12 As primary antibody, anti-FCoV mouse monoclonal IgG2A (FIPV3-70; Linaris) was used.

    Article Title: Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis
    Article Snippet: IHC was performed using clone FIPV3-70 antibody (Linaris Medizinische Produkte GmbH) on formalin-fixed, paraffin-embedded tissue sections.

    Article Title: Immune homeostasis and regulation of the interferon pathway require myeloid-derived Regnase-3
    Article Snippet: Staining antibodies were anti-B220 (RA3-6B2, rat-IgG2a; BD), anti-CD3 (SP7, rabbit IgG; Zytomed), anti-F4/80 (BM8, rat-IgG2a; Linaris), anti-MHC-II (M5/114.15.2, rat IgG; Novus Biologicals), anti-CD68 (Ab125212, rabbit IgG; Abcam) anti-ki67 (SP6, rabbit IgG; Thermo Fisher Scientific), and anti-Tyr701-phospho-STAT1 (58D6, rabbit IgG; Cell Signaling).

    Article Title: Antigens under cover—The preservation and demasking of selected antigens for successful poststaining immunocytochemistry of effusion, brain smears, and lymph node aspirates
    Article Snippet: Feline Coronavirus , Monoclonal Mouse Clone FIPV3‐70, MAK2194 , FIPV type 1 and 2 , Nucleocapsid , Linaris GmbH, Dossenheim, Germany.

    Immunohistochemistry:

    Article Title: Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis
    Article Snippet: .. IHC was performed using an FIPV3-70 monoclonal antibody (Linaris Medizinische Produkte GmbH, Dossenheim, Germany) on formalin-fixed, paraffin-embedded tissue sections as described previously [ ]. .. For signal detection, diaminobenzidine (DAB) staining was implemented (DAB Substrate Kit; Vector Laboratories, Burlingame, CA, USA).

    Article Title: Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis.
    Article Snippet: .. IHC was performed using an FIPV3-70 monoclonal antibody (Linaris Medizinische Produkte GmbH, Dossenheim, Germany) on formalin-fixed, paraffin-embedded tissue sections as described previously [33]. .. For signal detection, diaminobenzidine (DAB) staining Viruses 2022, 14, 1671 3 of 14 was implemented (DAB Substrate Kit; Vector Laboratories, Burlingame, CA, USA).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis
    Article Snippet: .. IHC was performed using an FIPV3-70 monoclonal antibody (Linaris Medizinische Produkte GmbH, Dossenheim, Germany) on formalin-fixed, paraffin-embedded tissue sections as described previously [ ]. .. For signal detection, diaminobenzidine (DAB) staining was implemented (DAB Substrate Kit; Vector Laboratories, Burlingame, CA, USA).

    Article Title: Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis.
    Article Snippet: .. IHC was performed using an FIPV3-70 monoclonal antibody (Linaris Medizinische Produkte GmbH, Dossenheim, Germany) on formalin-fixed, paraffin-embedded tissue sections as described previously [33]. .. For signal detection, diaminobenzidine (DAB) staining Viruses 2022, 14, 1671 3 of 14 was implemented (DAB Substrate Kit; Vector Laboratories, Burlingame, CA, USA).



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    The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Scientific Reports

    Article Title: The dual actions of miRNA16a in restricting Bovine Coronavirus replication through downregulation of Furin and enhancing the host immune response

    doi: 10.1038/s41598-024-80708-4

    Figure Lengend Snippet: The overexpression of the bovine miRNA16a inhibited BCoV replication on the viral genome copy numbers and the viral infectivity levels. ( A ) In silico prediction of miRNA16atargeting the BCoV spike gene at two different sites. The folding energy represents the binding energy of the miRNA with the target region. ( B ) Multiple sequence alignment shows that the miRNA16a/Spike binding region (indicated in red box) is conserved among nine different BCoV/Ent and BCoV/Resp isolates. ( C ) qRT-PCR analysis demonstrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected MDBK cells. ( D ) qRT-PCR analysis illustrating the genome viral load of BCoV in scrambled- and miRNA16a-transfected BEC cells. ( E ) Viral plaque assay indicating the infectivity level of the BCoV enteric isolate in scrambled- and miRNA16a-transfected MDBK cells. ( F ) Western blot analysis of BCoV-nucleocapsid (BCoV-N) and BCoV-spike (BCoV-S) in the MDBK cells transfected with scrambled or miRNA16a. ( G ) Western blot band density of the BCoV-N protein normalized to that of β-actin in the MDBK cells. ( H ) Western blot band density of the BCoV-S protein normalized to that of β-actin in the MDBK cells. ( I ) BEC cells were transfected with miRNA-Scr and miRNA16a, and western blot analysis was used to assess the protein expression of BCoV-N and BCoV-S. ( J ) Western blot band density of BCoV-N protein normalized to that of β-actin in BEC cells. ( K ) Western blot band density of BCoV-S protein normalized to that of β-actin in BEC cells. All the experiments were performed in triplicate. The significance of the data was determined by one-way ANOVA with Dunnett’s multiple comparison test and indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: The primary antibodies were used to detect the expression levels of the BCoV-nucleocapsid mouse anti-bovine monoclonal (clone: FIPV3-70; Cat. No. MA1-82,189), BCoV-spike rabbit anti-bovine polyclonal (cat. no. PA5-117562), and β-actin rabbit anti-bovine polyclonal (Catalogue number: PA1-46296) antibodies were purchased from Invitrogen.

    Techniques: Over Expression, Infection, In Silico, Binding Assay, Sequencing, Quantitative RT-PCR, Transfection, Viral Plaque Assay, Western Blot, Expressing, Comparison