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ont expwsh004xl nebnext ultra ii q5 master mix  (New England Biolabs)


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    Structured Review

    New England Biolabs ont expwsh004xl nebnext ultra ii q5 master mix
    Ont Expwsh004xl Nebnext Ultra Ii Q5 Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/exp-wsh004-xl/NEBNext+Ultra+II+Q5+Master+Mix/pm41247050-127-72-74
    Average 99 stars, based on 1786 article reviews
    ont expwsh004xl nebnext ultra ii q5 master mix - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens
    Article Snippet: .. For indexing PCR, the reaction was prepared with 0.75 μl of 10 μM SR primer, 0.75 μl of 10 μM indexing primer, 5 μl of cDNA, 6 μl of water, 12.5 μl of 2× NEBNext Ultra II Q5 Master Mix (NEB, M0544L) in total volume of 25 μl. ..

    Article Title: Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing
    Article Snippet: The product was amplified by up to 24 cycles of PCR using sequencing platform (Illumina) adaptor primers with unique dual indexing (UDI; e.g. E6440L, NEB). .. We used a NEBNext Q5U Master Mix (M0544S, NEB) with a spike-in of 7.5 μL 100X SYBR Gold (S11494, Thermo Fisher Scientific) to monitor the PCR curve on a QuantStudio 3 real-time PCR thermocycler ( A28567 , Thermo Fisher Scientific). ..

    Article Title: Pervasive Cas9 expression driven by mammalian promoter activity in E. coli affects representation of CRISPR sgRNA libraries.
    Article Snippet: .. All other sequencing library preparations (Fig4C, Fig6D,E) were performed by setting up 8 20uL PCR reactions (PCR1) using the NEB Next Ultra II Q5 Mastermix (NEB, catalogue number M0544X). .. Samples were sent to the UBC Sequencing Consortium (USC) for quality check, pooling, and sequencing on Illumina MiSeq using the Nano v2 kit with 20% PhiX spike in.

    Article Title: A mutational scar-based genome-wide map of DNA double-strand break repair.
    Article Snippet: .. The first round of PCR reactions was performed using NEBNext Ultra II Q5 Master Mix (NEB, Catalogue# M0544), containing 5μg genomic DNA, 0.2μM Fwd oligo 5’- TAGTGAACGGATCGGCACTG-3’, 0.2μM Rev oligo 5’-CTCCCCTACCCGGTAGAATTG-3’ per 50μl total reaction volume and run on a thermocycler with the following program: (1) 98oC for 3’, (2) 18 cycles of 98oC for 10”, 62oC for 30”, 72oC for 45”, (3) 72oC for 5’. .. Following PCR1 all parallel reactions were pooled and at least 600μl was purified using magnetic AMPure XP Beads (Beckman Coulter, Catalogue# A63882) in a 0.8X ratio and eluted in MQ.

    Article Title: Loop extrusion dynamics and cooperative activation independently regulate enhancer-driven transcription
    Article Snippet: .. BAC baits were PCR amplified using 5’ biotinylated forward and reverse primers (Suppl. table 3) in the following reaction mix: 10 μl 2X NEBNext UltraTM II Q5 Master Mix (NEB, M0544S), 1 μl of each 10 μM bio-primer, 2 μl adapter-ligated BAC DNA, 1X EvaGreen. ..

    Article Title: Coevolution and synchronized evolutionary rates in aphid dual endosymbiosis
    Article Snippet: Fragments were end-repaired, 3’-adenylated, and NEXTflex PCR free barcodes adapters (Bioo Scientific, USA) were added by using NEBNext® Ultra II DNA library prep kit for Illumina (New England Biolabs, USA). .. Ligation products were then purified by Ampure XP (Beckman Coulter, USA) and DNA fragments (>200 bp) were PCR-amplified (2 PCR reactions, 12 cycles), using Illumina adapter-specific primers and NEBNext ® Ultra II Q5 Master Mix (NEB). .. After library profile analysis by Agilent 2100 Bioanalyser (Agilent Technologies, USA) and qPCR quantification, using the KAPA Library Quantification Kit for Illumina Libraries (Kapa Biosystems, USA), the libraries were sequenced, using 251 bp paired-end reads chemistry on a HiSeq2500 Illumina sequencer.

    Real-time Polymerase Chain Reaction:

    Article Title: Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing
    Article Snippet: The product was amplified by up to 24 cycles of PCR using sequencing platform (Illumina) adaptor primers with unique dual indexing (UDI; e.g. E6440L, NEB). .. We used a NEBNext Q5U Master Mix (M0544S, NEB) with a spike-in of 7.5 μL 100X SYBR Gold (S11494, Thermo Fisher Scientific) to monitor the PCR curve on a QuantStudio 3 real-time PCR thermocycler ( A28567 , Thermo Fisher Scientific). ..

    Sequencing:

    Article Title: Pervasive Cas9 expression driven by mammalian promoter activity in E. coli affects representation of CRISPR sgRNA libraries.
    Article Snippet: .. All other sequencing library preparations (Fig4C, Fig6D,E) were performed by setting up 8 20uL PCR reactions (PCR1) using the NEB Next Ultra II Q5 Mastermix (NEB, catalogue number M0544X). .. Samples were sent to the UBC Sequencing Consortium (USC) for quality check, pooling, and sequencing on Illumina MiSeq using the Nano v2 kit with 20% PhiX spike in.

    BAC Assay:

    Article Title: Loop extrusion dynamics and cooperative activation independently regulate enhancer-driven transcription
    Article Snippet: .. BAC baits were PCR amplified using 5’ biotinylated forward and reverse primers (Suppl. table 3) in the following reaction mix: 10 μl 2X NEBNext UltraTM II Q5 Master Mix (NEB, M0544S), 1 μl of each 10 μM bio-primer, 2 μl adapter-ligated BAC DNA, 1X EvaGreen. ..

    Amplification:

    Article Title: Loop extrusion dynamics and cooperative activation independently regulate enhancer-driven transcription
    Article Snippet: .. BAC baits were PCR amplified using 5’ biotinylated forward and reverse primers (Suppl. table 3) in the following reaction mix: 10 μl 2X NEBNext UltraTM II Q5 Master Mix (NEB, M0544S), 1 μl of each 10 μM bio-primer, 2 μl adapter-ligated BAC DNA, 1X EvaGreen. ..

    Ligation:

    Article Title: Coevolution and synchronized evolutionary rates in aphid dual endosymbiosis
    Article Snippet: Fragments were end-repaired, 3’-adenylated, and NEXTflex PCR free barcodes adapters (Bioo Scientific, USA) were added by using NEBNext® Ultra II DNA library prep kit for Illumina (New England Biolabs, USA). .. Ligation products were then purified by Ampure XP (Beckman Coulter, USA) and DNA fragments (>200 bp) were PCR-amplified (2 PCR reactions, 12 cycles), using Illumina adapter-specific primers and NEBNext ® Ultra II Q5 Master Mix (NEB). .. After library profile analysis by Agilent 2100 Bioanalyser (Agilent Technologies, USA) and qPCR quantification, using the KAPA Library Quantification Kit for Illumina Libraries (Kapa Biosystems, USA), the libraries were sequenced, using 251 bp paired-end reads chemistry on a HiSeq2500 Illumina sequencer.

    Purification:

    Article Title: Coevolution and synchronized evolutionary rates in aphid dual endosymbiosis
    Article Snippet: Fragments were end-repaired, 3’-adenylated, and NEXTflex PCR free barcodes adapters (Bioo Scientific, USA) were added by using NEBNext® Ultra II DNA library prep kit for Illumina (New England Biolabs, USA). .. Ligation products were then purified by Ampure XP (Beckman Coulter, USA) and DNA fragments (>200 bp) were PCR-amplified (2 PCR reactions, 12 cycles), using Illumina adapter-specific primers and NEBNext ® Ultra II Q5 Master Mix (NEB). .. After library profile analysis by Agilent 2100 Bioanalyser (Agilent Technologies, USA) and qPCR quantification, using the KAPA Library Quantification Kit for Illumina Libraries (Kapa Biosystems, USA), the libraries were sequenced, using 251 bp paired-end reads chemistry on a HiSeq2500 Illumina sequencer.

    other:

    Article Title: Protocol for the generation of DDT signaling reporter cell line for CRISPR screening
    Article Snippet: NEBNext ultra II Q5 master mix , 25 μL.



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    Image Search Results


    Figures 2

    Journal: Cell Genomics

    Article Title: Long-read sequencing of diagnosis and post-therapy medulloblastoma reveals complex rearrangement patterns and epigenetic signatures

    doi: 10.1016/j.xgen.2023.100281

    Figure Lengend Snippet: Figures 2

    Article Snippet: Flow cell wash kit XL , Oxford Nanopore Technologies , EXP_WSH004-XL.

    Techniques: Ligation, Sequencing, Plasmid Preparation, Imaging, Software