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60x snp type reagent  (fluidigm)


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    Structured Review

    fluidigm 60x snp type reagent
    60x Snp Type Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dynamic+array+sample+assay+loading+reagent+kit/SNP+Type+Reagent/pmc10453920-103-53-57
    Average 94 stars, based on 9 article reviews
    60x snp type reagent - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera”
    Article Snippet: .. Sample Mixes were constituted of 1.9 μL of grape DNA at 20 ng/μL added to 2.6 μL of a sample pre-mix made of: - 472.5 μL of Biotium 2X Fast Probe Master Mix (Biotium, PN 31005); - 47.25 μL of 20X SNP Type Sample Loading Reagent (Fluidigm, PN 100-3425); - 15.75 μL of 60X SNP Type Reagent (Fluidigm, PN 100-3402); - 5.67 μL of ROX 50X (Life Technologies, PN 12223-012) at 25 μM; - 10.08 μL of PCR-certified water. ..

    Article Title: Discriminating populations of Atlantic herring mixing in the Norwegian Sea feeding ground using single nucleotide polymorphisms (SNPs)
    Article Snippet: .. The PCR on the 96.96 dynamic array was carried out in nl volumes, with 5 μl of each of the 96 SNP Type Assays loaded on the right of the array (7.5 μM allele specific primers [ASP1 and ASP2, forward primers with sequence tags attached, one for each fluorophore] and 20 μM LSP, 2× Assay Loading Reagent and PCR grade water); sample assays were loaded on the left of the array (5 μl total volume; 2.5 μl Biotium 2× Fast Probe Master Mix [Fluidigm], 0.25 μl 20× SNP Type sample loading reagent [Fluidigm], 0.083 μl 60× SNP Type reagent [Fluidigm], 0.03 μl ROX [Life Technologies], PCR grade water and 2.1 μl of the diluted PreAmp DNA). .. The arrays were primed and loaded with the 96.96 IFC controller, and after loading, the chip was placed in a BioMark HD System (Fluidigm) for PCR cycling according to the manufacturer’s instructions.

    Sequencing:

    Article Title: Discriminating populations of Atlantic herring mixing in the Norwegian Sea feeding ground using single nucleotide polymorphisms (SNPs)
    Article Snippet: .. The PCR on the 96.96 dynamic array was carried out in nl volumes, with 5 μl of each of the 96 SNP Type Assays loaded on the right of the array (7.5 μM allele specific primers [ASP1 and ASP2, forward primers with sequence tags attached, one for each fluorophore] and 20 μM LSP, 2× Assay Loading Reagent and PCR grade water); sample assays were loaded on the left of the array (5 μl total volume; 2.5 μl Biotium 2× Fast Probe Master Mix [Fluidigm], 0.25 μl 20× SNP Type sample loading reagent [Fluidigm], 0.083 μl 60× SNP Type reagent [Fluidigm], 0.03 μl ROX [Life Technologies], PCR grade water and 2.1 μl of the diluted PreAmp DNA). .. The arrays were primed and loaded with the 96.96 IFC controller, and after loading, the chip was placed in a BioMark HD System (Fluidigm) for PCR cycling according to the manufacturer’s instructions.

    Suspension:

    Article Title: Tobacco smoking as an endocrine disrupting chemical: An assessment through biological monitoring
    Article Snippet: PCR reactions were performed on an Arktik Thermal Cycler (Thermo Scientific, Rockford, IL) with the following cycling conditions: 10 min at 95°C, followed by 14 cycles of a 2-step amplification profile of 15 s at 95°C and 4 min at 60°C. .. STA products were 100-fold diluted in DNA suspension buffer and 2.5 μL of each product was combined with 3.0 μL of 2X Maxima® Probe/ROX qPCR Master Mix (Fermentas, St. Leon-Rot, Germany), 0.3 μL of SNPtype 20 X Sample Loading Reagent (Fluidigm), 0.1 μL of SNPtype Reagent (Fluidigm), and 0.1 μL of nuclease-free water. .. In parallel, 1 μL of each SNPtype assay was mixed with 2.5 μL of 2 X Assay Loading Reagent (Fluidigm) and 1.5 μL of nuclease-free water and loaded into the Fluidigm 96.96 Dynamic Genotyping Arrays.

    Real-time Polymerase Chain Reaction:

    Article Title: Tobacco smoking as an endocrine disrupting chemical: An assessment through biological monitoring
    Article Snippet: PCR reactions were performed on an Arktik Thermal Cycler (Thermo Scientific, Rockford, IL) with the following cycling conditions: 10 min at 95°C, followed by 14 cycles of a 2-step amplification profile of 15 s at 95°C and 4 min at 60°C. .. STA products were 100-fold diluted in DNA suspension buffer and 2.5 μL of each product was combined with 3.0 μL of 2X Maxima® Probe/ROX qPCR Master Mix (Fermentas, St. Leon-Rot, Germany), 0.3 μL of SNPtype 20 X Sample Loading Reagent (Fluidigm), 0.1 μL of SNPtype Reagent (Fluidigm), and 0.1 μL of nuclease-free water. .. In parallel, 1 μL of each SNPtype assay was mixed with 2.5 μL of 2 X Assay Loading Reagent (Fluidigm) and 1.5 μL of nuclease-free water and loaded into the Fluidigm 96.96 Dynamic Genotyping Arrays.



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