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lf032 deposited data rna microarray ncbi geo geo  (Genecopoeia)


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    Genecopoeia lf032 deposited data rna microarray ncbi geo geo
    Lf032 Deposited Data Rna Microarray Ncbi Geo Geo, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 96/100, based on 592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/Secrete-Pair+Dual+Luminescence+Assay+Kit/pm31543404-181-32-30
    Average 96 stars, based on 592 article reviews
    lf032 deposited data rna microarray ncbi geo geo - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Transduction:

    Article Title: Identification of Parkinson’s disease-associated regulatory variants in human dopaminergic neurons reveals modulators of SCARB2 and BAG3 expression
    Article Snippet: .. Supernatant of cells transduced with the luciferase vectors were collected 48 hours post-transduction and luciferase levels were measured using the Secreted-Pair Dual Luminescence assay kit (GeneCopoeia, LF032) according to the manufacturer’s instructions. .. After two passages post-thawing, iPSCs TH-Rep1 maintained in mTeSRTM Plus medium (StemCell TM Technologies, 100-0276) were nucleofected with a mix of 3 plasmids containing pCMV-PE2, pU6-pegRNA_SNP and pE3b-BAG3, or pmaxGFP TM control vector (Lonza), using the 4D-Nucleofector TM Core + X Unit (Lonza) instrument and the P3 Primary Cell Kit (Lonza V4XP-3024) (program CA137).

    Luciferase:

    Article Title: Identification of Parkinson’s disease-associated regulatory variants in human dopaminergic neurons reveals modulators of SCARB2 and BAG3 expression
    Article Snippet: .. Supernatant of cells transduced with the luciferase vectors were collected 48 hours post-transduction and luciferase levels were measured using the Secreted-Pair Dual Luminescence assay kit (GeneCopoeia, LF032) according to the manufacturer’s instructions. .. After two passages post-thawing, iPSCs TH-Rep1 maintained in mTeSRTM Plus medium (StemCell TM Technologies, 100-0276) were nucleofected with a mix of 3 plasmids containing pCMV-PE2, pU6-pegRNA_SNP and pE3b-BAG3, or pmaxGFP TM control vector (Lonza), using the 4D-Nucleofector TM Core + X Unit (Lonza) instrument and the P3 Primary Cell Kit (Lonza V4XP-3024) (program CA137).

    Article Title: Inhibition of miR-181c-5p Rescues Diabetes-Impaired Angiogenesis in Ischemia and Wound Healing.
    Article Snippet: Diabetes 2026;75(6):1–14 | https://doi.org/10.2337/db24-0867 D o w n l o a d e d f r o m h t t p : / / d i a b e t e s j o u r n a l s . o r g / d i a b e t e s / a r t i c l e - p d f / d o i / 1 0 .. 2 3 3 7 / d b 2 4 - 0 8 6 7 / 8 6 0 6 8 2 / d b 2 4 0 8 6 7 . p d f b y g u e s t o n 0 3 A p r i l 2 0 2 6 P H A R M A C O L O G Y A N D T H E R A P E U T IC S

    Luminescence Assay:

    Article Title: Identification of Parkinson’s disease-associated regulatory variants in human dopaminergic neurons reveals modulators of SCARB2 and BAG3 expression
    Article Snippet: .. Supernatant of cells transduced with the luciferase vectors were collected 48 hours post-transduction and luciferase levels were measured using the Secreted-Pair Dual Luminescence assay kit (GeneCopoeia, LF032) according to the manufacturer’s instructions. .. After two passages post-thawing, iPSCs TH-Rep1 maintained in mTeSRTM Plus medium (StemCell TM Technologies, 100-0276) were nucleofected with a mix of 3 plasmids containing pCMV-PE2, pU6-pegRNA_SNP and pE3b-BAG3, or pmaxGFP TM control vector (Lonza), using the 4D-Nucleofector TM Core + X Unit (Lonza) instrument and the P3 Primary Cell Kit (Lonza V4XP-3024) (program CA137).

    Article Title: Inhibition of miR-181c-5p Rescues Diabetes-Impaired Angiogenesis in Ischemia and Wound Healing.
    Article Snippet: Diabetes 2026;75(6):1–14 | https://doi.org/10.2337/db24-0867 D o w n l o a d e d f r o m h t t p : / / d i a b e t e s j o u r n a l s . o r g / d i a b e t e s / a r t i c l e - p d f / d o i / 1 0 .. 2 3 3 7 / d b 2 4 - 0 8 6 7 / 8 6 0 6 8 2 / d b 2 4 0 8 6 7 . p d f b y g u e s t o n 0 3 A p r i l 2 0 2 6 P H A R M A C O L O G Y A N D T H E R A P E U T IC S

    Article Title: Tumor-suppressive role of miR-216b impacts cell cycle regulation in high-grade glioma.
    Article Snippet: .. This data was further strengthened by GO enrichment analyses, showing mitotic cell cycle pathways (including CDK4, Supplementary Table 6) among the overlapping top hits (blue dot) according to miR-216b target gene prediction (green dots) but also based on differentially regulated genes upon miRNA manipulation in our cell models (purple dots) (Fig. 5b). miR-216b targets proteins involved in cell cycle regulation in glioma cells To test if CDK4 is a direct target of miR-216b in glioma cells, a dual-luciferase reporter assay was performed using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia). ..

    Article Title: Tumor-suppressive role of miR-216b impacts cell cycle regulation in high-grade glioma
    Article Snippet: .. To test if CDK4 is a direct target of miR-216b in glioma cells, a dual-luciferase reporter assay was performed using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia). ..

    Article Title: Breast Cancer-Derived Extracellular Vesicle miR-425-5p (miR-425) Promotes Brain Metastasis via Activating Astrocytes Through the Novel miR-425-ZNF24-CCL8 Signaling Axis.
    Article Snippet: Mouse brain tissues were fixed in acetone, washed with PBS-T (0.3%), incubated with mouse-mouse blocking reagent (ScyTek, Logan, UT, USA; cat. #MTM015) for 30 min, incubated with blocking (5% goat serum/PBS-T) for 1 h, and incubated with primary antibodies (see above) overnight at 4 ◦C. .. The Secrete-Pair Dual Luminescence Assay Kit (GeneCopoeia; cat. #LF032) was used following the manufacturer’s instructions. .. All slides were imaged at 4× or 20× using the Keyence BZ-X810 microscopes (cat. # BZ-X810 Keyence, Osaka, Japan).

    Negative Control:

    Article Title: Inhibition of miR-181c-5p Rescues Diabetes-Impaired Angiogenesis in Ischemia and Wound Healing.
    Article Snippet: Diabetes 2026;75(6):1–14 | https://doi.org/10.2337/db24-0867 D o w n l o a d e d f r o m h t t p : / / d i a b e t e s j o u r n a l s . o r g / d i a b e t e s / a r t i c l e - p d f / d o i / 1 0 .. 2 3 3 7 / d b 2 4 - 0 8 6 7 / 8 6 0 6 8 2 / d b 2 4 0 8 6 7 . p d f b y g u e s t o n 0 3 A p r i l 2 0 2 6 P H A R M A C O L O G Y A N D T H E R A P E U T IC S

    Activity Assay:

    Article Title: Inhibition of miR-181c-5p Rescues Diabetes-Impaired Angiogenesis in Ischemia and Wound Healing.
    Article Snippet: Diabetes 2026;75(6):1–14 | https://doi.org/10.2337/db24-0867 D o w n l o a d e d f r o m h t t p : / / d i a b e t e s j o u r n a l s . o r g / d i a b e t e s / a r t i c l e - p d f / d o i / 1 0 .. 2 3 3 7 / d b 2 4 - 0 8 6 7 / 8 6 0 6 8 2 / d b 2 4 0 8 6 7 . p d f b y g u e s t o n 0 3 A p r i l 2 0 2 6 P H A R M A C O L O G Y A N D T H E R A P E U T IC S

    Reporter Assay:

    Article Title: Tumor-suppressive role of miR-216b impacts cell cycle regulation in high-grade glioma.
    Article Snippet: .. This data was further strengthened by GO enrichment analyses, showing mitotic cell cycle pathways (including CDK4, Supplementary Table 6) among the overlapping top hits (blue dot) according to miR-216b target gene prediction (green dots) but also based on differentially regulated genes upon miRNA manipulation in our cell models (purple dots) (Fig. 5b). miR-216b targets proteins involved in cell cycle regulation in glioma cells To test if CDK4 is a direct target of miR-216b in glioma cells, a dual-luciferase reporter assay was performed using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia). ..

    Article Title: Tumor-suppressive role of miR-216b impacts cell cycle regulation in high-grade glioma
    Article Snippet: .. To test if CDK4 is a direct target of miR-216b in glioma cells, a dual-luciferase reporter assay was performed using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia). ..

    other:

    Article Title: Breast Cancer-Derived Extracellular Vesicle miR-425-5p (miR-425) Promotes Brain Metastasis via Activating Astrocytes Through the Novel miR-425-ZNF24-CCL8 Signaling Axis
    Article Snippet: Control and ZNF24 transfected cell lysates were immunoprecipitated using the ZNF24 antibody (scbt; cat. #sc-393359), or the normal mouse IgG provided in the EZ-ChIP Kit.



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