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deposited data raw gene microarray data  (TaKaRa)


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    TaKaRa deposited data raw gene microarray data
    Deposited Data Raw Gene Microarray Data, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/TB+Green+Premix+Ex+Taq/pm30067981-234-203-201
    Average 99 stars, based on 10970 article reviews
    deposited data raw gene microarray data - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Heterologous production of epothilones in Streptomyces albus J1074 via promoter engineering of the biosynthetic gene cluster and precursor supply enhancement
    Article Snippet: Genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis were performed using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio Inc., Shiga, Japan; cat. no. RR047A). .. Real-time PCR was carried out on a StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA, USA) using TB Green Premix Ex Taq (Takara Bio Inc., cat. no. RR420A), following the manufacturer's protocol. ..

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: Total RNA was isolated from SG tissue using TRIzolTM LS Reagent (Invitrogen, Cat. No. 10296010) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from 500 to 1000 ng of total RNA using PrimeScript RT Master Mix (TaKaRa, Cat. No. RR036A), and quantitative PCR was performed with TB Green® Premix Ex TaqTM (TaKaRa, Cat. No. RR420A) on a real-time PCR system using gene-specific primers for VEGF, HGF, CD31, and vWF, with GAPDH as the internal reference gene. ..

    Article Title: Synergistic osteogenesis, angiogenesis, and immune reprogramming by a metal-phenolic functionalized electrospun fibrous membrane for alveolar bone regeneration
    Article Snippet: .. Quantitative real-time PCR (RT-qPCR) was then executed using a TB Green Premix Ex Taq kit (Takara, RR420A, Japan) to measure the expression level of osteogenesis-related genes. ..

    Article Title: Screening of a quinonoid compounds library identifies decylubiquinone as an antioxidant and anti-apoptotic agent against glucocorticoid-induced osteoporosis via CD39/CD73/adenosine axis
    Article Snippet: .. Total RNA was extracted from cells or tissues using a FastPure Cell/Tissue Total RNA Isolation Kit V2 (RC112, Vazyme, Nanjing, Jiangsu, China). cDNA was synthesized from 1 μg total RNA using a RevertAid First Strand cDNA Synthesis Kit (K1622, Thermo, Rockford, IL, USA). qPCR was performed on a StepOne Plus PCR system (Applied Biosystems, Waltham, MA, USA) using TB Green Premix (RR420A, TaKaRa, Kusatsu, Shiga, Japan) and primers listed in . ..

    Article Title: Dietary serine supplementation improves growth performance and intestinal immunity and modulates gut microbiota in chronic oxidative stress-induced weaned piglets
    Article Snippet: .. Quantitative real-time PCR was performed using a CFX96 Real-Time PCR System (Bio-Rad Laboratories Inc., Hercules, CA, USA) with SYBR Premix Ex Taq (Cat. No. RR420A, Takara Biomedical Technology Co., Ltd., Beijing, China), following the manufacturer's instructions. ..

    Quantitative RT-PCR:

    Article Title: Thermoreversible cell-derived extracellular matrix only hydrogel (CEOgel): Development, characterization, and applications
    Article Snippet: Total RNA was extracted using an RNA extraction kit (QIAGEN, Hilden, Germany) following the manufacturer's protocol. cDNA was synthesized from the extracted RNA using SuperScriptTM VILOTM Master Mix (Invitrogen, Grand Island, NY, USA). .. RT-qPCR was performed with TB Green® Premix Ex TaqTM (TaKaRa, Shiga, Japan) on an ABI 7500 system (Applied Biosystems, Foster City, CA, USA) using gene-specific primers. ..

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: In vitro and in vivo anti-piroplasm activity of PBT2 against Theileria annulata and Babesia microti
    Article Snippet: .. RT-qPCR was performed using TB Green Premix Ex Taq (TaKaRa) according to the manufacturer's instructions with QuantStudio 5 instrument (Applied Biosystems, USA). ..

    Reverse Transcription:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    cDNA Synthesis:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: Screening of a quinonoid compounds library identifies decylubiquinone as an antioxidant and anti-apoptotic agent against glucocorticoid-induced osteoporosis via CD39/CD73/adenosine axis
    Article Snippet: .. Total RNA was extracted from cells or tissues using a FastPure Cell/Tissue Total RNA Isolation Kit V2 (RC112, Vazyme, Nanjing, Jiangsu, China). cDNA was synthesized from 1 μg total RNA using a RevertAid First Strand cDNA Synthesis Kit (K1622, Thermo, Rockford, IL, USA). qPCR was performed on a StepOne Plus PCR system (Applied Biosystems, Waltham, MA, USA) using TB Green Premix (RR420A, TaKaRa, Kusatsu, Shiga, Japan) and primers listed in . ..

    Synthesized:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: Total RNA was isolated from SG tissue using TRIzolTM LS Reagent (Invitrogen, Cat. No. 10296010) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from 500 to 1000 ng of total RNA using PrimeScript RT Master Mix (TaKaRa, Cat. No. RR036A), and quantitative PCR was performed with TB Green® Premix Ex TaqTM (TaKaRa, Cat. No. RR420A) on a real-time PCR system using gene-specific primers for VEGF, HGF, CD31, and vWF, with GAPDH as the internal reference gene. ..

    Article Title: Screening of a quinonoid compounds library identifies decylubiquinone as an antioxidant and anti-apoptotic agent against glucocorticoid-induced osteoporosis via CD39/CD73/adenosine axis
    Article Snippet: .. Total RNA was extracted from cells or tissues using a FastPure Cell/Tissue Total RNA Isolation Kit V2 (RC112, Vazyme, Nanjing, Jiangsu, China). cDNA was synthesized from 1 μg total RNA using a RevertAid First Strand cDNA Synthesis Kit (K1622, Thermo, Rockford, IL, USA). qPCR was performed on a StepOne Plus PCR system (Applied Biosystems, Waltham, MA, USA) using TB Green Premix (RR420A, TaKaRa, Kusatsu, Shiga, Japan) and primers listed in . ..

    Expressing:

    Article Title: Synergistic osteogenesis, angiogenesis, and immune reprogramming by a metal-phenolic functionalized electrospun fibrous membrane for alveolar bone regeneration
    Article Snippet: .. Quantitative real-time PCR (RT-qPCR) was then executed using a TB Green Premix Ex Taq kit (Takara, RR420A, Japan) to measure the expression level of osteogenesis-related genes. ..

    Isolation:

    Article Title: Screening of a quinonoid compounds library identifies decylubiquinone as an antioxidant and anti-apoptotic agent against glucocorticoid-induced osteoporosis via CD39/CD73/adenosine axis
    Article Snippet: .. Total RNA was extracted from cells or tissues using a FastPure Cell/Tissue Total RNA Isolation Kit V2 (RC112, Vazyme, Nanjing, Jiangsu, China). cDNA was synthesized from 1 μg total RNA using a RevertAid First Strand cDNA Synthesis Kit (K1622, Thermo, Rockford, IL, USA). qPCR was performed on a StepOne Plus PCR system (Applied Biosystems, Waltham, MA, USA) using TB Green Premix (RR420A, TaKaRa, Kusatsu, Shiga, Japan) and primers listed in . ..

    Polymerase Chain Reaction:

    Article Title: Screening of a quinonoid compounds library identifies decylubiquinone as an antioxidant and anti-apoptotic agent against glucocorticoid-induced osteoporosis via CD39/CD73/adenosine axis
    Article Snippet: .. Total RNA was extracted from cells or tissues using a FastPure Cell/Tissue Total RNA Isolation Kit V2 (RC112, Vazyme, Nanjing, Jiangsu, China). cDNA was synthesized from 1 μg total RNA using a RevertAid First Strand cDNA Synthesis Kit (K1622, Thermo, Rockford, IL, USA). qPCR was performed on a StepOne Plus PCR system (Applied Biosystems, Waltham, MA, USA) using TB Green Premix (RR420A, TaKaRa, Kusatsu, Shiga, Japan) and primers listed in . ..



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