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bone resorption assay kit csr-bra-48kit  (Cosmo Bio USA)

 
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    Cosmo Bio USA bone resorption assay kit csr-bra-48kit
    EN’s role in osteoclast differentiation and function. Raw264.7 cells were pre-treated with EN (10 and 20 μg/mL) for 1 h, followed by RANKL (40 ng/mL). ( A ) The area of <t>resorption</t> pits formed by mature osteoclast cells was evaluated using a bone resorption assay and visualized under a light microscope at 40× magnification (Scale bar. 100 μm). ( B ) The pit area measurements, quantified in pixels, are depicted in a bar graph. ( C ) The formation of actin rings in mature osteoclast cells was observed using a LSM 700 laser scanning confocal microscope, highlighting F-actin staining (upper line) and DAPI staining (bottom line) at 100× magnification (Scale bar = 200 μm). Data represent mean ± SEM ( n ≥ 3). One-way analysis of variance (ANOVA) was utilized, followed by the Bonferroni post hoc test for pairwise comparisons. *** p < 0.001; n.s. = not significant. DMSO: dimethyl sulfoxide.
    Bone Resorption Assay Kit Csr Bra 48kit, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/csr-bra-48kit/pmc11052068-145-8-13?v=Cosmo+Bio+USA
    Average 90 stars, based on 1 article reviews
    bone resorption assay kit csr-bra-48kit - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "A 70% Ethanol Neorhodomela munita Extract Attenuates RANKL-Induced Osteoclast Activation and H 2 O 2 -Induced Osteoblast Apoptosis In Vitro"

    Article Title: A 70% Ethanol Neorhodomela munita Extract Attenuates RANKL-Induced Osteoclast Activation and H 2 O 2 -Induced Osteoblast Apoptosis In Vitro

    Journal: Molecules

    doi: 10.3390/molecules29081741

    EN’s role in osteoclast differentiation and function. Raw264.7 cells were pre-treated with EN (10 and 20 μg/mL) for 1 h, followed by RANKL (40 ng/mL). ( A ) The area of resorption pits formed by mature osteoclast cells was evaluated using a bone resorption assay and visualized under a light microscope at 40× magnification (Scale bar. 100 μm). ( B ) The pit area measurements, quantified in pixels, are depicted in a bar graph. ( C ) The formation of actin rings in mature osteoclast cells was observed using a LSM 700 laser scanning confocal microscope, highlighting F-actin staining (upper line) and DAPI staining (bottom line) at 100× magnification (Scale bar = 200 μm). Data represent mean ± SEM ( n ≥ 3). One-way analysis of variance (ANOVA) was utilized, followed by the Bonferroni post hoc test for pairwise comparisons. *** p < 0.001; n.s. = not significant. DMSO: dimethyl sulfoxide.
    Figure Legend Snippet: EN’s role in osteoclast differentiation and function. Raw264.7 cells were pre-treated with EN (10 and 20 μg/mL) for 1 h, followed by RANKL (40 ng/mL). ( A ) The area of resorption pits formed by mature osteoclast cells was evaluated using a bone resorption assay and visualized under a light microscope at 40× magnification (Scale bar. 100 μm). ( B ) The pit area measurements, quantified in pixels, are depicted in a bar graph. ( C ) The formation of actin rings in mature osteoclast cells was observed using a LSM 700 laser scanning confocal microscope, highlighting F-actin staining (upper line) and DAPI staining (bottom line) at 100× magnification (Scale bar = 200 μm). Data represent mean ± SEM ( n ≥ 3). One-way analysis of variance (ANOVA) was utilized, followed by the Bonferroni post hoc test for pairwise comparisons. *** p < 0.001; n.s. = not significant. DMSO: dimethyl sulfoxide.

    Techniques Used: Light Microscopy, Microscopy, Staining



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    EN’s role in osteoclast differentiation and function. Raw264.7 cells were pre-treated with EN (10 and 20 μg/mL) for 1 h, followed by RANKL (40 ng/mL). ( A ) The area of <t>resorption</t> pits formed by mature osteoclast cells was evaluated using a bone resorption assay and visualized under a light microscope at 40× magnification (Scale bar. 100 μm). ( B ) The pit area measurements, quantified in pixels, are depicted in a bar graph. ( C ) The formation of actin rings in mature osteoclast cells was observed using a LSM 700 laser scanning confocal microscope, highlighting F-actin staining (upper line) and DAPI staining (bottom line) at 100× magnification (Scale bar = 200 μm). Data represent mean ± SEM ( n ≥ 3). One-way analysis of variance (ANOVA) was utilized, followed by the Bonferroni post hoc test for pairwise comparisons. *** p < 0.001; n.s. = not significant. DMSO: dimethyl sulfoxide.
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    Image Search Results


    Inhibitory effect on osteoclast differentiation and bone resorption. (A) RAW264.7 cells were cultured in DMEM high glucose for 5 days in the presence of RANKL (50 ng/mL) with CBD, taurine, and both. (B) RAW264.7 cells were seeded in CaP-coated 48-well plates and treated with 50 ng/mL RANKL in the presence of CBD, taurine, and both. (C) TRAP-positive multinucleated cells (TRAP+ MNCs) were counted after fixation and staining for TRAP. (D) Pit area was measured. The results are presented as means ± SD (n=3). *** p <0.001 indicates significant differences from CBD+taurine treated groups with CBD and taurine alone.

    Journal: Biomolecules & Therapeutics

    Article Title: Combination of Cannabidiol with Taurine Synergistically Treated Periodontitis in Rats

    doi: 10.4062/biomolther.2024.104

    Figure Lengend Snippet: Inhibitory effect on osteoclast differentiation and bone resorption. (A) RAW264.7 cells were cultured in DMEM high glucose for 5 days in the presence of RANKL (50 ng/mL) with CBD, taurine, and both. (B) RAW264.7 cells were seeded in CaP-coated 48-well plates and treated with 50 ng/mL RANKL in the presence of CBD, taurine, and both. (C) TRAP-positive multinucleated cells (TRAP+ MNCs) were counted after fixation and staining for TRAP. (D) Pit area was measured. The results are presented as means ± SD (n=3). *** p <0.001 indicates significant differences from CBD+taurine treated groups with CBD and taurine alone.

    Article Snippet: The bone resorption pit assay was carried out using a Bone Resorption Test Kit (CSF-BRA-48 KIT, Cosmo Bio) in plates coated with calcium phosphate.

    Techniques: Cell Culture, Staining

    EN’s role in osteoclast differentiation and function. Raw264.7 cells were pre-treated with EN (10 and 20 μg/mL) for 1 h, followed by RANKL (40 ng/mL). ( A ) The area of resorption pits formed by mature osteoclast cells was evaluated using a bone resorption assay and visualized under a light microscope at 40× magnification (Scale bar. 100 μm). ( B ) The pit area measurements, quantified in pixels, are depicted in a bar graph. ( C ) The formation of actin rings in mature osteoclast cells was observed using a LSM 700 laser scanning confocal microscope, highlighting F-actin staining (upper line) and DAPI staining (bottom line) at 100× magnification (Scale bar = 200 μm). Data represent mean ± SEM ( n ≥ 3). One-way analysis of variance (ANOVA) was utilized, followed by the Bonferroni post hoc test for pairwise comparisons. *** p < 0.001; n.s. = not significant. DMSO: dimethyl sulfoxide.

    Journal: Molecules

    Article Title: A 70% Ethanol Neorhodomela munita Extract Attenuates RANKL-Induced Osteoclast Activation and H 2 O 2 -Induced Osteoblast Apoptosis In Vitro

    doi: 10.3390/molecules29081741

    Figure Lengend Snippet: EN’s role in osteoclast differentiation and function. Raw264.7 cells were pre-treated with EN (10 and 20 μg/mL) for 1 h, followed by RANKL (40 ng/mL). ( A ) The area of resorption pits formed by mature osteoclast cells was evaluated using a bone resorption assay and visualized under a light microscope at 40× magnification (Scale bar. 100 μm). ( B ) The pit area measurements, quantified in pixels, are depicted in a bar graph. ( C ) The formation of actin rings in mature osteoclast cells was observed using a LSM 700 laser scanning confocal microscope, highlighting F-actin staining (upper line) and DAPI staining (bottom line) at 100× magnification (Scale bar = 200 μm). Data represent mean ± SEM ( n ≥ 3). One-way analysis of variance (ANOVA) was utilized, followed by the Bonferroni post hoc test for pairwise comparisons. *** p < 0.001; n.s. = not significant. DMSO: dimethyl sulfoxide.

    Article Snippet: The bone resorption assay was conducted using a bone resorption assay kit (CSR-BRA-48KIT; COSMOBIO, Tokyo, Japan), following the manufacturer’s instructions with slight modifications.

    Techniques: Light Microscopy, Microscopy, Staining