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cdna  (New England Biolabs)


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    Structured Review

    New England Biolabs cdna
    Transcriptome analysis of 59 selected genes associated with oral chemosensation, displayed as a heatmap showing mean fold changes in gene expression of the SSB-fed diet groups in relation to the respective water-fed group (=1) in the form of a color code. The gene expression was analyzed using one customized <t>cDNA</t> microarray per group from <t>pooled</t> <t>RNA</t> samples of the CV from mice that received either a standard diet (chow, n = 10–11) or Western-type diet (WD, n = 7–8) with water (Water) or a sugar-sweetened beverage (SSB) as a drink for 24 weeks.
    Cdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complementary+dna+(cdna)+microarray/LunaScript+RT+SuperMix/pmc08837962-92-10-17
    Average 97 stars, based on 1260 article reviews
    cdna - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Long-Term Consumption of a Sugar-Sweetened Soft Drink in Combination with a Western-Type Diet Is Associated with Morphological and Molecular Changes of Taste Markers Independent of Body Weight Development in Mice"

    Article Title: Long-Term Consumption of a Sugar-Sweetened Soft Drink in Combination with a Western-Type Diet Is Associated with Morphological and Molecular Changes of Taste Markers Independent of Body Weight Development in Mice

    Journal: Nutrients

    doi: 10.3390/nu14030594

    Transcriptome analysis of 59 selected genes associated with oral chemosensation, displayed as a heatmap showing mean fold changes in gene expression of the SSB-fed diet groups in relation to the respective water-fed group (=1) in the form of a color code. The gene expression was analyzed using one customized cDNA microarray per group from pooled RNA samples of the CV from mice that received either a standard diet (chow, n = 10–11) or Western-type diet (WD, n = 7–8) with water (Water) or a sugar-sweetened beverage (SSB) as a drink for 24 weeks.
    Figure Legend Snippet: Transcriptome analysis of 59 selected genes associated with oral chemosensation, displayed as a heatmap showing mean fold changes in gene expression of the SSB-fed diet groups in relation to the respective water-fed group (=1) in the form of a color code. The gene expression was analyzed using one customized cDNA microarray per group from pooled RNA samples of the CV from mice that received either a standard diet (chow, n = 10–11) or Western-type diet (WD, n = 7–8) with water (Water) or a sugar-sweetened beverage (SSB) as a drink for 24 weeks.

    Techniques Used: Expressing, Microarray, Western Blot

    Related Articles

    Isolation:

    Article Title: A two-component RUBY reporter system to assess plant virus infection.
    Article Snippet: .. Total RNA isolated from cDNA derived infections or subsequent mechanical passages were reverse-transcribed using LunaScript RT SuperMix (NEB, Ipswich, MA USA) and first strand-synthesis used for subsequent PCR analysis. ..

    Derivative Assay:

    Article Title: A two-component RUBY reporter system to assess plant virus infection.
    Article Snippet: .. Total RNA isolated from cDNA derived infections or subsequent mechanical passages were reverse-transcribed using LunaScript RT SuperMix (NEB, Ipswich, MA USA) and first strand-synthesis used for subsequent PCR analysis. ..

    Reverse Transcription:

    Article Title: A two-component RUBY reporter system to assess plant virus infection.
    Article Snippet: .. Total RNA isolated from cDNA derived infections or subsequent mechanical passages were reverse-transcribed using LunaScript RT SuperMix (NEB, Ipswich, MA USA) and first strand-synthesis used for subsequent PCR analysis. ..

    Article Title: Remnant virus proteins sustain vascular inflammation post coronavirus infection in mice and humans.
    Article Snippet: .. Reverse transcription was performed using the GoScript Reverse Transcription System (Promega) or LunaScript RT SuperMix (New England Biolabs). .. Quantitative PCR was conducted using a C1000 Thermal Cycler (Bio-Rad), with MCoV infection quantified as described previously (22).

    Article Title: Phosphorylation of YBX1 in the Kidneys is Altered in Legumain Knockout-Mice.
    Article Snippet: The isolated RNA concentration was determined by using a NanoDrop One/One spectrophotometer (Thermo Scientific). .. In the reverse transcription reaction, 1 mg of total RNA was used with LunaScript RT Supermix (New England Biolabs). .. For the RT-qPCR reaction, 10 ng of cDNA per well was used with KAPA SYBR FAST (Merck) master mix.

    Article Title: NCX2 Targeting as a Neuroprotective Strategy Against Oxaliplatin-Induced Peripheral Neurotoxicity.
    Article Snippet: .. Reverse transcription was performed using LunaScript RT SuperMix 5× (New England Biolabs, Ipswich, MA, USA) and a Mastercycler nexus X2 PCR Thermal Cycler (Eppendorf, Hamburg, Germany), starting from 100 ng of RNA for each sample. .. qPCR was performed using Luna Universal qPCR Master Mix 2× (New England Biolabs, Ipswich, MA, USA) on a QuantStudio 7 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA).

    Polymerase Chain Reaction:

    Article Title: A two-component RUBY reporter system to assess plant virus infection.
    Article Snippet: .. Total RNA isolated from cDNA derived infections or subsequent mechanical passages were reverse-transcribed using LunaScript RT SuperMix (NEB, Ipswich, MA USA) and first strand-synthesis used for subsequent PCR analysis. ..

    Article Title: NCX2 Targeting as a Neuroprotective Strategy Against Oxaliplatin-Induced Peripheral Neurotoxicity.
    Article Snippet: .. Reverse transcription was performed using LunaScript RT SuperMix 5× (New England Biolabs, Ipswich, MA, USA) and a Mastercycler nexus X2 PCR Thermal Cycler (Eppendorf, Hamburg, Germany), starting from 100 ng of RNA for each sample. .. qPCR was performed using Luna Universal qPCR Master Mix 2× (New England Biolabs, Ipswich, MA, USA) on a QuantStudio 7 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA).

    Synthesized:

    Article Title: The Inv(16) Oncogene CBFB::MYH11 is Required for the Survival of Leukemia Cells in the Blood and Spleen, but not the Bone Marrow
    Article Snippet: RNA was extracted using Trizol (Thermo Scientific, Waltham, MA, USA) as described previously [ , ]. .. cDNA was synthesized and amplified using Luna RT Supermix and Luna Universal qPCR kit (New England Biolabs, Ipswich, MA, USA) per manufacturer’s protocol. ..

    Article Title: Tissue-Specific Expression and Laying-Performance Responsiveness of FXR1 PRL, GnRH2, and TFPI2 in Helmeted Guinea Fowl
    Article Snippet: .. First-strand cDNA was synthesized from total RNA extracted from brain, liver and ovary tissues using Luna Script RT Super Mix (New England Biolabs, USA). .. The reverse-transcription reaction was performed in a final volume of 20 μL containing 4 μL of 5X Luna Script RT Super Mix and the appropriate amount of RNA template, with nuclease-free water added to obtain the final reaction volume.

    Article Title: Direct cell-to-cell transmission of retrotransposons.
    Article Snippet: RT-qPCR analysis of transposon expression Total RNA was extracted from five pairs of dissected ovaries per biological replicate using an in-house RNA purification kit on an automated KingFisher Duo Prime system (Thermo Fisher Scientific), including DNase treatment. .. cDNA was synthesized from 500 ng of total RNA using LunaScript RT SuperMix (New England Biolabs) according to the manufacturer’s instructions. .. qPCR was performed using Luna Universal qPCR Master Mix (New England Biolabs) on a Bio-Rad C1000 Touch Thermal Cycler realtime PCR system.

    Amplification:

    Article Title: The Inv(16) Oncogene CBFB::MYH11 is Required for the Survival of Leukemia Cells in the Blood and Spleen, but not the Bone Marrow
    Article Snippet: RNA was extracted using Trizol (Thermo Scientific, Waltham, MA, USA) as described previously [ , ]. .. cDNA was synthesized and amplified using Luna RT Supermix and Luna Universal qPCR kit (New England Biolabs, Ipswich, MA, USA) per manufacturer’s protocol. ..

    Real-time Polymerase Chain Reaction:

    Article Title: The Inv(16) Oncogene CBFB::MYH11 is Required for the Survival of Leukemia Cells in the Blood and Spleen, but not the Bone Marrow
    Article Snippet: RNA was extracted using Trizol (Thermo Scientific, Waltham, MA, USA) as described previously [ , ]. .. cDNA was synthesized and amplified using Luna RT Supermix and Luna Universal qPCR kit (New England Biolabs, Ipswich, MA, USA) per manufacturer’s protocol. ..



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