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anti type iv collagen  (Rockland Immunochemicals)


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    Structured Review

    Rockland Immunochemicals anti type iv collagen
    Anti Type Iv Collagen, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagen+iv/AFF%2EPUR%2EANTI-COLLAGEN+TYPE+IV/pmc07463893-66-31-34
    Average 93 stars, based on 24 article reviews
    anti type iv collagen - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Control:

    Article Title: The latent dedifferentiation capacity of newt limb muscles is unleashed by a combination of metamorphosis and body growth
    Article Snippet: .. Primary antibodies were mouse monoclonal anti-Pax7 antibody (1:200; PAX7-c; Developmental Studies Hybridoma Bank, University of Iowa, Iowa, IA, USA), mouse monoclonal anti-myosin heavy chain antibody (1:200; MF20-c; Developmental Studies Hybridoma Bank) and rabbit polyclonal anti-PCNA antibody (1:500; ab18197; Abcam, Tokyo, Japan) , and for control, rabbit polyclonal anti-collagen type IV antibody (1:500; 600-401-106-0.1; Rockland Immunochemicals, Gilbertsville, PA 19525, USA). .. Secondary antibodies were goat anti-mouse IgG (H + L) antibody conjugated with Alexa Fluor 405 (1:200; A-31553; Thermo Fisher Scientific) and goat anti-rabbit IgG (H + L) antibody conjugated with Alexa Fluor 405 (1:500; ab175652; Abcam).

    Article Title: The latent dedifferentiation capacity of newt limb muscles is unleashed by a combination of metamorphosis and body growth.
    Article Snippet: .. Primary antibodies were mouse monoclonal anti-Pax7 antibody (1:200; PAX7-c; Developmental Studies Hybridoma Bank, University of Iowa, Iowa, IA, USA), mouse monoclonal anti-myosin heavy chain antibody (1:200; MF20-c; Developmental Studies Hybridoma Bank) and rabbit polyclonal anti-PCNA antibody (1:500; ab18197; Abcam, Tokyo, Japan)20, and for control, rabbit polyclonal anti-collagen type IV antibody (1:500; 600-401-106-0.1; Rockland Immunochemicals, Gilbertsville, PA 19525, USA). .. Secondary antibodies were goat anti-mouse IgG (H + L) antibody conjugated with Alexa Fluor 405 (1:200; A-31553; Thermo Fisher Scientific) and goat anti-rabbit IgG (H + L) antibody conjugated with Alexa Fluor 405 (1:500; ab175652; Abcam).

    Staining:

    Article Title: Efficacy of Deferoxamine Mesylate in Serum and Serum-Free Media: Adult Ventral Root Schwann Cell Survival Following Hydrogen Peroxide-Induced Cell Death.
    Article Snippet: .. We stained SCs with GFAP (Chicken, 1:1000, Aves GFAP, Davis, CA, USA), P75 (Rabbit, 1:400, Bioss BS-0161R, Woburn, MA, USA), HiF1α (Rabbit, 1:500, Sigma Aldrich SAB5701087, St. Louis, MO, USA), and Collagen IV (Rabbit, 1:400, Rockland 600-401-106-0.1, Pottstown, PA, USA) primary antibodies. .. Secondary antibodies used were from Jackson Immuno (West Grove, PA) and used at 1:800; we used . c tic f eri e tal esi a c a cell c lt res.Sche atic of the experits. i i ts f tr t ts r r i ll .r s t ti ri tfi l i ages of Sch ann cells 16 h following hydrogen peroxide challenge (B(III,IV)) or sham (B(I,II)) in serum-containing medium. (B(I,III)) shows a 20× image, and (B(II,IV)) shows a 40× image. (C) Representative brightfield images of Schwann cells 16 h following hydrogen peroxide challenge (C(III,IV)) or sham (C(I,II)) in serum-free medium. (C(I,III)) shows a 20× image and (C(II,IV)) shows a 40× image.

    Article Title: Efficacy of Deferoxamine Mesylate in Serum and Serum-Free Media: Adult Ventral Root Schwann Cell Survival Following Hydrogen Peroxide-Induced Cell Death
    Article Snippet: .. We stained SCs with GFAP (Chicken, 1:1000, Aves GFAP, Davis, CA, USA), P75 (Rabbit, 1:400, Bioss BS-0161R, Woburn, MA, USA), HiF1α (Rabbit, 1:500, Sigma Aldrich SAB5701087, St. Louis, MO, USA), and Collagen IV (Rabbit, 1:400, Rockland 600-401-106-0.1, Pottstown, PA, USA) primary antibodies. .. Secondary antibodies used were from Jackson Immuno (West Grove, PA, USA) and used at 1:800; we used donkey anti-chicken AlexaFluor 488 (703-546-155) and donkey anti-rabbit cy3 (711-166-152).

    Article Title: Keratinocyte-driven dermal collagen formation in the axolotl skin.
    Article Snippet: .. Immunofluorescence The following primary antibodies were used for immunofluorescence staining: rabbit anti-GFP pAb (MBL Cat# 598, 1:500, RRID:AB_591819), mouse anti-collagen type I (#C2456-2 ML, 1:500; Sigma), mouse antipan cytokeratin (AE1 + AE3) antibody (NBP2-29429, 1:100; Novus Biologicals), mouse anti-procollagen type I aminoterminal extension peptide (SP1.D8, 1:10; DSHB), rabbit anti-Laminin (L9393, 1:100; Sigma), rabbit anti-collagen type III alpha1 (N-terminal) (22734-1-AP, 1:500; Proteintech), rabbit anti-collagen type IV (600-401-106-0.1, 1:100; Rockland), rabbit anti-procollagen type I (orb389309, 1:100; Biorbyt) and mouse anti-collagen Type2 (II-II6B3C, 1:100; DSHB). ..

    Western Blot:

    Article Title: Myeloid EGFR deficiency accelerates recovery from AKI via macrophage efferocytosis and neutrophil apoptosis
    Article Snippet: .. List of antibody, source, application, and dilution Antibody Catalog Number Dilution Goat anti-EGFR Rabbit anti-pEGFR Rabbit anti-S100A9 Rat anti-Gr-1 Rabbit anti-MCL-1 Rabbit anti-Bax Mouse anti-α-Tubulin Rat anti-F4/80 Mouse anti-α-SMA Rabbit anti-Col IV Rabbit anti-Col I Anti-mouse CD11b (M1/70) Anti-mouse Ly6G (1A8) Anti-mouse Ly6c (HK1.4) Anti-mouse F4/80 (BM8) Sigma, E1281 Abcam, ab40815 ThermoFisher, PIPA146489 BioRad, MCAa2387 Cell signal technology, 94296S Cell signal technology, 5023T Cell signal technology, 3873 BioRad, MCA497 Sigma-Aldrich, A5228 Rockland, 6004011060.1 Rockland, 600-401-103-0.1 Biolegend 101226 Biolegend 127672 Biolegend 128024 Biolegend 123114 IF 1:50, WB 1:1000 IF 1:100 IF 1:100 IF1:100 IF 1:250, WB 1:1000 IF 1:100 WB 1:2000 IHC 1:100 IHC 1:500, WB1:2000 IHC 1:100 IHC 1:100 FC 0.2mg/ml FC 0.2mg/ml FC 0.2mg/ml FC 0.2mg/ml Anti-mouse CD45 (30-F11) Anti-cleaved caspase 3 Anti-C1q (JL-1) Rabbit anti-cleaved caspase 3 Rabbit anti-fibronectin Goat anti-KIM 1 Goat anti-NGAL Rabbit anti-CD68 Rabbit anti- Arginase 1 Mouse anti-β-actin Goat anti-PDGFR beta Biolegend 103149 R&D IC835G Thermo Fisher, MA1-40313 Cell signal technology, 9661S Sigma Aldrich, F3648 R&D, AF1817 R&D, AF1857 Abcan, ab125212 Novus, NBP1-32731 Sigma Aldrich, A 1978 R&D, AF1042 FC 0.1 mg/ml FC 0.2mg/ml FC 0.1mg/ml IF 1:50 WB 1:1000 WB 1:500 WB 1:500 IF 1:100 IF 1:100 WB 1:2000 WB 1:500 Table S3. .. List of gene probes (Applied Biosystems) Mouse primer Catalog Number Emr1 Mm00802529 Cd68 Mm03047343 Tnf Mm99999068 Il1b Mm00434228 Il6 Il11 Mm00446190 Mm00434162 Col1a1 Mm00801666 Fn1 Mm01256744 Col4a1 Mm01210125 Acta2 Mm01546133 Tgfb1 CD3 CD4 CD8a Il1α Mm00441726 Mm00442746 Mm00442754 Mm01182107 Mm00439621 Ctgf Mm01192933 Mrc1 Arg1 Ccl2 Ccl3 Egfr Il23a Ifng Vim Nos2 Irf5 Ym1 Il4 Il10 Gas6 Pros1 Mfge8 Mcl1 Mm01329362 Mm00475991 Mm00441242 Mm00441258 Mm00433023 Mm00518984 Mm01168134 Mm01333430 Mm00440502 Mm00496477 Mm00657889 Mm00515786 Mm00439616 Mm00490378 Mm01343426 Mm00500549 Mm01257351

    Immunohistochemistry:

    Article Title: Myeloid EGFR deficiency accelerates recovery from AKI via macrophage efferocytosis and neutrophil apoptosis
    Article Snippet: .. List of antibody, source, application, and dilution Antibody Catalog Number Dilution Goat anti-EGFR Rabbit anti-pEGFR Rabbit anti-S100A9 Rat anti-Gr-1 Rabbit anti-MCL-1 Rabbit anti-Bax Mouse anti-α-Tubulin Rat anti-F4/80 Mouse anti-α-SMA Rabbit anti-Col IV Rabbit anti-Col I Anti-mouse CD11b (M1/70) Anti-mouse Ly6G (1A8) Anti-mouse Ly6c (HK1.4) Anti-mouse F4/80 (BM8) Sigma, E1281 Abcam, ab40815 ThermoFisher, PIPA146489 BioRad, MCAa2387 Cell signal technology, 94296S Cell signal technology, 5023T Cell signal technology, 3873 BioRad, MCA497 Sigma-Aldrich, A5228 Rockland, 6004011060.1 Rockland, 600-401-103-0.1 Biolegend 101226 Biolegend 127672 Biolegend 128024 Biolegend 123114 IF 1:50, WB 1:1000 IF 1:100 IF 1:100 IF1:100 IF 1:250, WB 1:1000 IF 1:100 WB 1:2000 IHC 1:100 IHC 1:500, WB1:2000 IHC 1:100 IHC 1:100 FC 0.2mg/ml FC 0.2mg/ml FC 0.2mg/ml FC 0.2mg/ml Anti-mouse CD45 (30-F11) Anti-cleaved caspase 3 Anti-C1q (JL-1) Rabbit anti-cleaved caspase 3 Rabbit anti-fibronectin Goat anti-KIM 1 Goat anti-NGAL Rabbit anti-CD68 Rabbit anti- Arginase 1 Mouse anti-β-actin Goat anti-PDGFR beta Biolegend 103149 R&D IC835G Thermo Fisher, MA1-40313 Cell signal technology, 9661S Sigma Aldrich, F3648 R&D, AF1817 R&D, AF1857 Abcan, ab125212 Novus, NBP1-32731 Sigma Aldrich, A 1978 R&D, AF1042 FC 0.1 mg/ml FC 0.2mg/ml FC 0.1mg/ml IF 1:50 WB 1:1000 WB 1:500 WB 1:500 IF 1:100 IF 1:100 WB 1:2000 WB 1:500 Table S3. .. List of gene probes (Applied Biosystems) Mouse primer Catalog Number Emr1 Mm00802529 Cd68 Mm03047343 Tnf Mm99999068 Il1b Mm00434228 Il6 Il11 Mm00446190 Mm00434162 Col1a1 Mm00801666 Fn1 Mm01256744 Col4a1 Mm01210125 Acta2 Mm01546133 Tgfb1 CD3 CD4 CD8a Il1α Mm00441726 Mm00442746 Mm00442754 Mm01182107 Mm00439621 Ctgf Mm01192933 Mrc1 Arg1 Ccl2 Ccl3 Egfr Il23a Ifng Vim Nos2 Irf5 Ym1 Il4 Il10 Gas6 Pros1 Mfge8 Mcl1 Mm01329362 Mm00475991 Mm00441242 Mm00441258 Mm00433023 Mm00518984 Mm01168134 Mm01333430 Mm00440502 Mm00496477 Mm00657889 Mm00515786 Mm00439616 Mm00490378 Mm01343426 Mm00500549 Mm01257351

    Blocking Assay:

    Article Title: IPSC-derived organoid-sourced skin cells enable functional 3D skin modeling of recessive dystrophic epidermolysis bullosa
    Article Snippet: .. Permeabilization was performed with 0.1% Triton X for 10 min, followed by blocking at room temperature with 5% donkey serum and 8% bovine serum albumin in PBS for 1 h. The following primary antibodies were used and incubated overnight at 4°C: keratin 14 (BioLegend #906004), keratin 10 (BioLegend #905404), keratin 5 (BioLegend #905904), Ki67 (Abcam #ab16667), involucrin (Abcam #ab27495), DSG1 (Thermo Fisher # MA191590 ), loricrin (Abcam #ab188994), filaggrin (Abcam #ab218395), vimentin (Santa Cruz #sc-6260), fibronectin (Santa Cruz #sc-73611), collagen I (Abcam #ab6308), collagen III (Abcam #ab6310), collagen IV (Rockland #6004011060.1), collagen VII (EMD Millipore #234192), CD90 (BioLegend #328110), FAP (R&D Systems #AF3715-SP), elastin (Abcam #ab21610), enactin (Abcam #ab254325), laminin α5 (EMD Millipore #MABT39). ..

    Incubation:

    Article Title: IPSC-derived organoid-sourced skin cells enable functional 3D skin modeling of recessive dystrophic epidermolysis bullosa
    Article Snippet: .. Permeabilization was performed with 0.1% Triton X for 10 min, followed by blocking at room temperature with 5% donkey serum and 8% bovine serum albumin in PBS for 1 h. The following primary antibodies were used and incubated overnight at 4°C: keratin 14 (BioLegend #906004), keratin 10 (BioLegend #905404), keratin 5 (BioLegend #905904), Ki67 (Abcam #ab16667), involucrin (Abcam #ab27495), DSG1 (Thermo Fisher # MA191590 ), loricrin (Abcam #ab188994), filaggrin (Abcam #ab218395), vimentin (Santa Cruz #sc-6260), fibronectin (Santa Cruz #sc-73611), collagen I (Abcam #ab6308), collagen III (Abcam #ab6310), collagen IV (Rockland #6004011060.1), collagen VII (EMD Millipore #234192), CD90 (BioLegend #328110), FAP (R&D Systems #AF3715-SP), elastin (Abcam #ab21610), enactin (Abcam #ab254325), laminin α5 (EMD Millipore #MABT39). ..

    Immunofluorescence:

    Article Title: Keratinocyte-driven dermal collagen formation in the axolotl skin.
    Article Snippet: .. Immunofluorescence The following primary antibodies were used for immunofluorescence staining: rabbit anti-GFP pAb (MBL Cat# 598, 1:500, RRID:AB_591819), mouse anti-collagen type I (#C2456-2 ML, 1:500; Sigma), mouse antipan cytokeratin (AE1 + AE3) antibody (NBP2-29429, 1:100; Novus Biologicals), mouse anti-procollagen type I aminoterminal extension peptide (SP1.D8, 1:10; DSHB), rabbit anti-Laminin (L9393, 1:100; Sigma), rabbit anti-collagen type III alpha1 (N-terminal) (22734-1-AP, 1:500; Proteintech), rabbit anti-collagen type IV (600-401-106-0.1, 1:100; Rockland), rabbit anti-procollagen type I (orb389309, 1:100; Biorbyt) and mouse anti-collagen Type2 (II-II6B3C, 1:100; DSHB). ..



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