ice cold lysis buffer for co ip (Wanleibio)

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Wanleibio ice cold lysis buffer for co ip
Ice Cold Lysis Buffer For Co Ip, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/co+ip+lysis+buffer/buffer+lysis+ripa/pmc12561918-73-24-29
Average 86 stars, based on 1 article reviews

ice cold lysis buffer for co ip - by Bioz Stars, 2026-09

86/100 stars

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Modification:

Article Title: Protective effects of dopamine against non-alcoholic steatohepatitis via inhibiting p65 pathways in vivo and in vitro
Article Snippet: .. The following materials were used in the present study: Dulbecco modified eagle’s medium (DMEM)-high glucose (Sigma-Aldrich, USA), total cholesterol (TC), triglyceride (TG) assay kit (Nanjing Jiancheng Bioengineering Institute, China), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Aladdin Bio-Chem Technology, China), antibiotics and fetal bovine serum (FBS) (Gibco, USA), isopropanol, oleate, palmitate, oil red O and defatted bovine serum albumin (St Louis, MO, USA), high fat diet (carbohydrates, 20%; fat, 60%; protein, 20%; D12492; Huafukang Bioscience; Beijing, China), methionine-choline deficient diet (TP3005G; TrophicDiet, Nantong, China), radio immunoprecipitation assay (RIPA) buffer (Wanleibio, China), polyvinylidene fluoride (PVDF) membrane (Millipore Corporation, Bedford, MA, USA), efficient chemiluminescence (ECL) plus kit (Biosharp; Labgic), antibody to p-p65, p65, F4/80, NLRP3, ASC, Caspase1, IL-1β, IL6 and IL18 (Wanleibio, Shenyang, China), β-actin (Bioworld Technology, Co., Ltd China), GSMD (Wuhan ColorfulGene Biological Technology Co. Ltd, China), horseradish peroxidase (HRP)-linked secondary antibody (Sangon Biotech, China), FITC Goat Anti-rabbit IgG H&L/FITC; Bioss, Beijing, China). ..

MTT Assay:

Radio Immunoprecipitation:

Membrane:

Western Blot:

Article Title: Relationship Between ACSL4-Mediated Ferroptosis and Chronic Obstructive Pulmonary Disease
Article Snippet: .. Western Blot Analysis Total proteins were extracted from tissues or cells using a lysis buffer (Wanleibio, WLA019, Shenyang, China). .. Proteins were quantified using a BCA protein assay kit (Wanleibio, WLA004, Shenyang, China).

Article Title: Pseudorabies Virus UL41 Hijacks IFN Response via JAK/STAT Pathway While Cellular TRIM21 Blocks it Through K48 Ubiquitination
Article Snippet: .. Briefly, cells were harvested and lysed in a cell lysis buffer for Western and IP (WanleiBio, Shenyang, China), supplemented with protease inhibitors (Sellcek, Houston, TX, USA). .. Protein was resolved by 10% SDS–PAGE and transferred onto a 0.45 μm PVDF membrane (Millipore, Billerica, MA, USA).

Lysis:

Article Title: Nrf2 deficiency in myeloid cells accelerates atherosclerosis by promoting the inflammatory response and impairing efferocytosis.
Article Snippet: .. Proteins were harvested using a lysis buffer (Wanleibio) and quantitated using a Bicinchoninic acid assay kit (TaKaRa Bio). ..

Article Title: The Ubiquitination of Mycobacterium tuberculosis Rv3717 Promotes Proteasomal Degradation of Interleukin Enhancer-Binding Factor
Article Snippet: .. The cells were placed in ice-cold RIPA lysis buffer (Wanlei Bio, Shenyang, China) with 1 mM PMSF; nuclear proteins and cytoplasmic proteins were isolated via a nuclear protein and cytoplasmic protein extraction kit (Wanlei Bio, Shenyang, China). .. The proteins were collected using centrifugation and quantified using BCA reagent (Wanlei Bio, Shenyang, China).

Article Title: Discovery of novel harmine derivatives as potent, selective, and brain permeable GSK3β inhibitors with effective In vivo anti-AD activity.
Article Snippet: Alzheimer’s disease (AD) is a neurodegenerative disorder with limited therapeutic options.. Glycogen synthase kinase 3β (GSK3β), a key enzyme in tau phosphorylation, is a promising therapeutic target for AD.. Herein, we employed a structure-based drug design strategy to develop a novel series of harmine derivatives as potent GSK3β inhibitors.

Article Title: The Ubiquitination of Mycobacterium tuberculosis Rv3717 Promotes Proteasomal Degradation of Interleukin Enhancer-Binding Factor
Article Snippet: HEK293T cells were transiently co-transfected with GV230::Flag_Rv3717 and GV230::HA_ILF2 or GV230::HA_UBC using Lipo8000TM Transfection Reagent (Beyotime Bio, Shanghai, China). .. The medium was replaced after 12 h, and the cells were harvested after 36 h. The cells were washed with PBS and lysed with ice-cold lysis buffer for Co-IP (Wanlei Bio, Shenyang, China) with 1 mM PMSF. ..

other:

Article Title: Pseudorabies Virus UL41 Hijacks IFN Response via JAK/STAT Pathway While Cellular TRIM21 Blocks it Through K48 Ubiquitination
Article Snippet: Then, cells were lysed in lysis buffer for Western blotting and immunoprecipitation (WanleiBio, Shenyang, China) supplemented with protease inhibitors (Sellcek, Houston, TX, USA) and RNase inhibitor (E125, Novoprotein, Jiangsu, China) for 15 min on ice.

Acid Assay:

Isolation:

Protein Extraction:

Co-Immunoprecipitation Assay:

Article Title: The Ubiquitination of Mycobacterium tuberculosis Rv3717 Promotes Proteasomal Degradation of Interleukin Enhancer-Binding Factor
Article Snippet: HEK293T cells were transiently co-transfected with GV230::Flag_Rv3717 and GV230::HA_ILF2 or GV230::HA_UBC using Lipo8000TM Transfection Reagent (Beyotime Bio, Shanghai, China). .. The medium was replaced after 12 h, and the cells were harvested after 36 h. The cells were washed with PBS and lysed with ice-cold lysis buffer for Co-IP (Wanlei Bio, Shenyang, China) with 1 mM PMSF. ..

Review