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pluriSelect Life Science UG Co KG red blood cell lysis buffer 60-00050-12
Red Blood Cell Lysis Buffer 60 00050 12, supplied by pluriSelect Life Science UG Co KG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/co+ip+lysis+buffer/red+blood+cell+lysis+buffer/pmc10276297-327-23-29
Average 90 stars, based on 1 article reviews
red blood cell lysis buffer 60-00050-12 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Red Blood Cell Lysis:

Article Title: Flk1 Deficiency and Hypoxia Synergistically Promote Endothelial Dysfunction, Vascular Remodeling, and Pulmonary Hypertension
Article Snippet: 1668 September 2023 Arterioscler Thromb Vasc Biol.. 2023;43:1668–1683.. DOI: 10.1161/ATVBAHA.123.319266 Arterioscler Thromb Vasc Biol is available at www.ahajournals.org/journal/atvb Correspondence to: Masaki Ieda, MD, PhD, Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

Article Title: Association of Gut Microbiome with Early Brain Injury After Subarachnoid Hemorrhage: an Experimental Study.
Article Snippet: The occurrence of early brain injury (EBI) following subarachnoid hemorrhage (SAH) is crucial in the prognosis of SAH; however, no effective treatment for EBI has been developed.. Gut microbiome (GM) composition influences the outcome of various diseases, including ischemic stroke.. Here, we evaluated whether prior GM alteration could prevent EBI following SAH.

Article Title: Astragalus root increases Treg and Th17 involvement in embryo implantation and pregnancy maintenance by decreasing CTLA-4 + Tregs.
Article Snippet: .. The spleen was extirpated under sterile conditions, minced in RPMI medium (RPMI-1640, FUJIFILM Wako Pure Chemical Corporation), filtered through 40 μm mesh and hemolyzed with red blood cell lysis buffer (pluriSelect Life Science). .. Splenocytes were suspended in RPMI medium containing 0.05 mM 2-mercaptoethanol (FUJIFILM Wako Pure Chemical Corporation), 10% heat-inactivated FBS (Gibco Fetal Bovine Serum, Qualified, ThermoFisher Scientific Inc), antibiotic and antimycotic (Penicillin-Streptomycin mixed stock solution, Nacalai Tesque Inc.) and were adjusted to 5 × 106 viable cells per milliliter.

Article Title: Disulfiram ameliorates bone loss in ovariectomized mice by suppressing osteoclastogenesis
Article Snippet: .. Red blood cells among BM cells were lysed using red blood cell lysis solution (pluriSelect, Leipzig, Germany). ..

Article Title: Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners.
Article Snippet: .. The filtered cell suspension was centrifuged at 300g for 10 min, and the cell pellet was incubated in 10 ml of cold Red Blood Cell Lysis Buffer (60-00050-12, pluriSelect) for 10 min in the refrigerator (2−8 C), followed by centrifugation at room temperature and at 300g for 10 min. ..

Article Title: Novel therapeutic strategies for injured endometrium: intrauterine transplantation of menstrual blood‑derived cells from infertile patients.
Article Snippet: .. Then, 10 ml of 1X RBC lysis buffer (pluriSelect, GER, catalog number 60-00050-12) was added and gently pipetted several times. ..

Article Title: Disulfiram ameliorates bone loss in ovariectomized mice by suppressing osteoclastogenesis.
Article Snippet: .. Red blood cells among BM cells were lysed using red blood cell lysis solution (pluriSelect, Leipzig, Germany). ..

Article Title: GM-CSF receptor/SYK/JNK/FOXO1/CD11c signaling promotes atherosclerosis.
Article Snippet: PMA (AG-CN2-0010; AdipoGen)-primed THP-1 and HL-60 cells were detached using cold PBS (-). .. Peripheral blood cells drawn from the inferior vena cava were incubated thrice with RBC lysis buffer (60-00050-11; pluriSelect, Leipzig, Germany) for 2 min to remove red blood cells. ..

Staining:

Article Title: Flk1 Deficiency and Hypoxia Synergistically Promote Endothelial Dysfunction, Vascular Remodeling, and Pulmonary Hypertension
Article Snippet: 1668 September 2023 Arterioscler Thromb Vasc Biol.. 2023;43:1668–1683.. DOI: 10.1161/ATVBAHA.123.319266 Arterioscler Thromb Vasc Biol is available at www.ahajournals.org/journal/atvb Correspondence to: Masaki Ieda, MD, PhD, Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

Sterility:

Article Title: Astragalus root increases Treg and Th17 involvement in embryo implantation and pregnancy maintenance by decreasing CTLA-4 + Tregs.
Article Snippet: .. The spleen was extirpated under sterile conditions, minced in RPMI medium (RPMI-1640, FUJIFILM Wako Pure Chemical Corporation), filtered through 40 μm mesh and hemolyzed with red blood cell lysis buffer (pluriSelect Life Science). .. Splenocytes were suspended in RPMI medium containing 0.05 mM 2-mercaptoethanol (FUJIFILM Wako Pure Chemical Corporation), 10% heat-inactivated FBS (Gibco Fetal Bovine Serum, Qualified, ThermoFisher Scientific Inc), antibiotic and antimycotic (Penicillin-Streptomycin mixed stock solution, Nacalai Tesque Inc.) and were adjusted to 5 × 106 viable cells per milliliter.

Suspension:

Article Title: Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners.
Article Snippet: .. The filtered cell suspension was centrifuged at 300g for 10 min, and the cell pellet was incubated in 10 ml of cold Red Blood Cell Lysis Buffer (60-00050-12, pluriSelect) for 10 min in the refrigerator (2−8 C), followed by centrifugation at room temperature and at 300g for 10 min. ..

Incubation:

Article Title: Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners.
Article Snippet: .. The filtered cell suspension was centrifuged at 300g for 10 min, and the cell pellet was incubated in 10 ml of cold Red Blood Cell Lysis Buffer (60-00050-12, pluriSelect) for 10 min in the refrigerator (2−8 C), followed by centrifugation at room temperature and at 300g for 10 min. ..

Article Title: GM-CSF receptor/SYK/JNK/FOXO1/CD11c signaling promotes atherosclerosis.
Article Snippet: PMA (AG-CN2-0010; AdipoGen)-primed THP-1 and HL-60 cells were detached using cold PBS (-). .. Peripheral blood cells drawn from the inferior vena cava were incubated thrice with RBC lysis buffer (60-00050-11; pluriSelect, Leipzig, Germany) for 2 min to remove red blood cells. ..

Centrifugation:

Article Title: Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners.
Article Snippet: .. The filtered cell suspension was centrifuged at 300g for 10 min, and the cell pellet was incubated in 10 ml of cold Red Blood Cell Lysis Buffer (60-00050-12, pluriSelect) for 10 min in the refrigerator (2−8 C), followed by centrifugation at room temperature and at 300g for 10 min. ..



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