lysis buffer (MedChemExpress)

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MedChemExpress lysis buffer
Lysis Buffer, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/co+ip+lysis+buffer/IP%2FCo-IP+Kit/pm41888318-216-12-15
Average 94 stars, based on 25 article reviews

lysis buffer - by Bioz Stars, 2026-09

94/100 stars

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Incubation:

Article Title: AMZ2 facilitates oral squamous cell carcinoma progression via USP10-mediated deubiquitination of SF3B1 and regulation of MCL-1 alternative splicing.
Article Snippet: Cells were lysed using immunoprecipitation lysis buffer (PC105, Epizyme Bio, Shanghai, China), and the supernatant was collected following centrifugation. .. The clarified lysate was incubated with the specified primary antibody overnight at 4 ◦C, followed by incubation with Protein A/G magnetic beads (HY-K0202K, MedChemExpress) for 4 h to capture the immune complexes. ..

Magnetic Beads:

Article Title: Endothelial SP1 lactylation promotes bronchopulmonary dysplasia via regulation of Cdkn1a expression.
Article Snippet: Protein–protein interaction (PPI) analysis of candidate genes was performed using the STRING database and visualized in Cytoscape. .. Immunoprecipitation was performed using an IP/Co-IP Kit with Protein A/G magnetic beads (MedChemExpress, USA) according to the manufacturer’s instructions. .. Cells were lysed in IP lysis buffer supplemented with protease inhibitors (MedChemExpress, USA), and the lysates were incubated with anti-SP1 antibody (Proteintech, China) or control IgG (Proteintech, China) together with Protein A/G magnetic beads.

Immunoprecipitation:

Activity Assay:

Article Title: Silencing PCSK9 reshapes the spatiotemporal activation of STING for safe and effective cancer immunotherapy
Article Snippet: After overnight culture, BMDCs were pretreated with 20 μg/ml siRNA siPCSK9 or siCtrl for 24 h and then treated with 400 μM Mn 2+ for 3 h. SURF4 Co-IP samples were prepared by Pierce TM Direct Magnetic IP/Co-IP Kit (Thermofisher, Cat# 88828) following previously reported methods with slight modifications . .. Briefly, cells were lysed by IP buffer in IP/Co-IP Kits supplemented with 1% 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS), and beads were washed by wash buffer IP/Co-IP Kits supplemented with 0.7% CHAPS (MCE, Cat# HY-15435) for stabilized protein-protein complexes and retained the biochemical activity of proteins in solution. ..

other:

Article Title: Lactobacillus paragasseri LPG-9 reduces placental inflammation in intrahepatic cholestasis of pregnancy by regulating TGR5 in mice
Article Snippet: For Co-IP of native proteins, the samples were completely homogenized with a lysis buffer (HY-K0202K-B, MCE, USA), the samples were centrifuged for 10 min at 12,000 rpm and 4 °C, and collected at −80 °C for subsequent testing.

Co-Immunoprecipitation Assay:

Article Title: Lactobacillus paragasseri LPG-9 reduces placental inflammation in intrahepatic cholestasis of pregnancy by regulating TGR5 in mice.
Article Snippet: .. For Co-IP of native proteins, the samples were completely homogenized with a lysis buffer (HY-K0202K-B, MCE, USA), the samples were centrifuged for 10 min at 12,000 rpm and 4 °C , and collected at -80 °C for subsequent testing. .. Next 500 μg of cell lysate was incubated overnight at 4 °C with mouse anti-TLR4 (1:100; 66350, Proteintech, USA) and rabbit antiTGR5 (1:50; A20778, ABclonal, China) antibodies, using IgG (1:1000) as a negative control.

Lysis:

Article Title: Lactobacillus paragasseri LPG-9 reduces placental inflammation in intrahepatic cholestasis of pregnancy by regulating TGR5 in mice.
Article Snippet: For Co-IP of overexpressed proteins, plasmids encoding N-terminally HA-tagged TGR5 and N-terminally FLAG-tagged TLR4 were constructed and co-transfected in HEK293T cells using lipo8000 (C0533, Beyotime, China). .. After 24 h, cells were lysed in a non-denaturing IP lysis buffer (HY-K0202K, MCE, USA). .. The cell lysate was incubated with anti-FLAG (HY-K0207, MCE, USA) and anti-HA magnetic beads (HY-K0201, MCE, USA) to immunoprecipitate FLAG-TLR4 and anti-HA, respectively, overnight at 4 °C.

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