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Proteintech clu
Clu, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clusterin+clu/Clusterin+Antibody/pmc12948417-249-25-26
Average 93 stars, based on 31 article reviews
clu - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Immunofluorescence:

Article Title: Acute Inflammation Drives Corneal Pathological Regeneration.
Article Snippet: All slides were imaged using a ZEISS LSM 800 confocal laser scanning microscope. .. The antibodies used for immunofluorescence are as follows: anti-KRT3 (no. ab68260, 1:200; Abcam, Cambridge, MA, USA), antiKRT12 (no. ab124975, 1:200; Abcam), anti-ALDH3A1 (no. GTX30042, 1:200; GeneTex, Inc., Irvine, CA, USA), anti-CLU (no. 12289-1-AP, 1:200; Proteintech, Rosemont, IL, USA), and anti-PAX6 (no. AMAB91372, 1:200; Sigma-Aldrich Corp., St. Louis, MO, USA). .. TUNEL staining was performed according to the manufacturer’s instructions (no. C1086; Beyotime Institute of Biotechnology, Jiangsu, China).

Article Title: Acute Inflammation Drives Corneal Pathological Regeneration
Article Snippet: All slides were imaged using a ZEISS LSM 800 confocal laser scanning microscope. .. The antibodies used for immunofluorescence are as follows: anti-KRT3 (no. ab68260, 1:200; Abcam, Cambridge, MA, USA), anti-KRT12 (no. ab124975, 1:200; Abcam), anti-ALDH3A1 (no. GTX30042, 1:200; GeneTex, Inc., Irvine, CA, USA), anti-CLU (no. 12289-1-AP, 1:200; Proteintech, Rosemont, IL, USA), and anti-PAX6 (no. AMAB91372, 1:200; Sigma-Aldrich Corp., St. Louis, MO, USA). .. TUNEL staining was performed according to the manufacturer's instructions (no. C1086; Beyotime Institute of Biotechnology, Jiangsu, China).

Article Title: α -Ketoglutarate protects against cartilage damage via epigenetically driven metabolic reprogramming in osteoarthritis models
Article Snippet: Each condition was performed with 4–6 replicates, and the analysis of data was performed using Wave software (Seahorse Bioscience). .. Immunofluorescence staining was carried out by incubation overnight at 4°C with antibodies against COL1A1 (Servicebio, GB11022-1; 1:200), Aspn (Affinity, DF13642; 1:100), Cilp (Huabio, ER1906-32; 1:100), Clu (Proteintech, 66109-1-Ig; 1:100), and Cnmd (Affinity, DF13715; 1:100). .. Alexa Fluor 594– or Cy3-conjugated secondary antibodies (Invitrogen) were added for 30 minutes, followed by staining with DAPI (Beyotime).

Incubation:

Article Title: (-)-Epigallocatechin-3-gallate promotes the dermal papilla cell proliferation and migration through the induction of VEGFA.
Article Snippet: Dermal papilla cells (DPCs) are crucial for the growth and development of hair follicles (HF). (− )-Epigallocatechin-3-gallate (EGCG) is the primary catechin identified in green tea, which has antioxidant effects and regulates cell activity.. This study demonstrates that EGCG could promote the proliferation of DPCs.. In addition, EGCG treatment significantly upregulated the expression of PCNA, CCND1, HIF-1α, VEGFA, and Bcl-2 mRNAs in DPCs, while significantly reducing the gene expression of Bax.

Article Title: Secreted Clusterin Inhibits Keloid Formation by Promoting Fibroblast Apoptosis
Article Snippet: .. Primary antibody of CLU (1:1200 dilution, 12289-1-AP from Proteintech) was applied and incubated for 15 minutes at room temperature. ..

Article Title: α -Ketoglutarate protects against cartilage damage via epigenetically driven metabolic reprogramming in osteoarthritis models
Article Snippet: Each condition was performed with 4–6 replicates, and the analysis of data was performed using Wave software (Seahorse Bioscience). .. Immunofluorescence staining was carried out by incubation overnight at 4°C with antibodies against COL1A1 (Servicebio, GB11022-1; 1:200), Aspn (Affinity, DF13642; 1:100), Cilp (Huabio, ER1906-32; 1:100), Clu (Proteintech, 66109-1-Ig; 1:100), and Cnmd (Affinity, DF13715; 1:100). .. Alexa Fluor 594– or Cy3-conjugated secondary antibodies (Invitrogen) were added for 30 minutes, followed by staining with DAPI (Beyotime).

FLAG-tag:

Article Title: Dichotomous roles of ACBD3 in NSCLC growth and metastasis
Article Snippet: We purchased SYBR Green, fetal bovine serum (FBS), Dulbecco’s minimal essential medium (DMEM), RPMI Media 1640, Alexa Fluor-tagged secondary antibodies, and DAPI from Life Technologies; puromycin from InvivoGene; paraformaldehyde from Electron Microscopy Sciences; Transwell and Matrigel-coated Boyden chambers from BD Biosciences; G418 from Corning; We obtained shRNAs against human ACBD3 (TRCN0000065218 and TRCN0000065219), human PI4KB (TRCN0000005692), and mouse ACBD3 (TRCN0000124734 and TRCN0000124738); siRNAs against human ACBD3 (SASI_Hs01_00052488 and SASI_Hs01_00052489), human PI4KB (SASI_Hs01_00149544), human SNAI1 (SASI_Hs01_00039785), and control siRNA (SIC002) from Sigma. .. We purchased primary antibodies against ACBD3 (sc-101277) from Santa Cruz; GM130 (#560066) from BD Transduction Laboratories; α-tubulin (#T9026) from Sigma; Flag tag (F3165) and EGFP (G6539) from Sigma; SNAI1 (#3879), PARP1 (#9542), and β-actin (#4970) from Cell Signaling; ZEB1 (21544-1-AP), STC2 (10314-1-AP), and CLU (12289-1-AP) from Proteintech; CDH1 (13–1700) from ThermoFisher Scientific; and VSVG (IE9F9) from Kerafast. .. Human lung cancer cell lines (A549, H1299, CALU1, CALU6, H358, H460, H441, H292, H358, H2122, H1792, and HCC827) and human embryonic kidney 293 T cells were obtained from the American Type Culture Collection.

Article Title: Dichotomous roles of ACBD3 in NSCLC growth and metastasis.
Article Snippet: We purchased SYBR Green, fetal bovine serum (FBS), Dulbecco’s minimal essential medium (DMEM), RPMI Media 1640, Alexa Fluor-tagged secondary antibodies, and DAPI from Life Technologies; puromycin from InvivoGene; paraformaldehyde from Electron Microscopy Sciences; Transwell and Matrigel-coated Boyden chambers from BD Biosciences; G418 from Corning; We obtained shRNAs against human ACBD3 (TRCN0000065218 and TRCN0000065219), human PI4KB (TRCN0000005692), and mouse ACBD3 (TRCN0000124734 and TRCN0000124738); siRNAs against human ACBD3 (SASI_Hs01_00052488 and SASI_Hs01_00052489), human PI4KB (SASI_Hs01_00149544), human SNAI1 (SASI_Hs01_00039785), and control siRNA (SIC002) from Sigma. .. We purchased primary antibodies against ACBD3 (sc-101277) from Santa Cruz; GM130 (#560066) from BD Transduction Laboratories; α-tubulin (#T9026) from Sigma; Flag tag (F3165) and EGFP (G6539) from Sigma; SNAI1 (#3879), PARP1 (#9542), and β-actin (#4970) from Cell Signaling; ZEB1 (21544-1-AP), STC2 (10314-1-AP), and CLU (12289-1-AP) from Proteintech; CDH1 (13–1700) from ThermoFisher Scientific; and VSVG (IE9F9) from Kerafast. .. Human lung cancer cell lines (A549, H1299, CALU1, CALU6, H358, H460, H441, H292, H358, H2122, H1792, and HCC827) and human embryonic kidney 293 T cells were obtained from the American Type Culture Collection.

Staining:

Article Title: α -Ketoglutarate protects against cartilage damage via epigenetically driven metabolic reprogramming in osteoarthritis models
Article Snippet: Each condition was performed with 4–6 replicates, and the analysis of data was performed using Wave software (Seahorse Bioscience). .. Immunofluorescence staining was carried out by incubation overnight at 4°C with antibodies against COL1A1 (Servicebio, GB11022-1; 1:200), Aspn (Affinity, DF13642; 1:100), Cilp (Huabio, ER1906-32; 1:100), Clu (Proteintech, 66109-1-Ig; 1:100), and Cnmd (Affinity, DF13715; 1:100). .. Alexa Fluor 594– or Cy3-conjugated secondary antibodies (Invitrogen) were added for 30 minutes, followed by staining with DAPI (Beyotime).



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