Journal: Bioactive Materials

Article Title: A catalytically active and recyclable bioelastomer inspired by metalloenzymes

doi: 10.1016/j.bioactmat.2026.02.053

Figure Lengend Snippet: Cytocompatibility of Cu-PIAS. ( A ) Viability, as measured by ATP content, of human umbilical vein endothelial cells cultured with media containing extracts of 15-Cu-PIAS, Latex (Positive), or PCL (Negative) over three days. ( B ) Representative images of cell morphology after 24 h exposure to material extract-containing media, visualized by Calcein AM (Green) staining. Additional staining with ethidium homodimer-1 (red) indicates the presence of non-viable cells.

Article Snippet: Briefly, endothelial growth medium supplemented with 5% fetal bovine serum, growth factors, ascorbic acid, and hydrocortisone (Promocell C-22121) was incubated at 37 °C for 72 h under agitation alone, or in the presence of 15-Cu-PIAS (6 cm 2 /mL), PCL (6 cm 2 /mL), or latex (3 cm 2 /mL) sheets cut into several 6 mm × 6 mm squares.

Techniques: Cell Culture, Staining

Journal: iScience

Article Title: RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion

doi: 10.1016/j.isci.2026.116333

Figure Lengend Snippet: rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in 5637 cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of WT cultured in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .

Article Snippet: 5637 cells (ATCC HTB-9) were purchased from American Type Culture Collection and cultured in RPMI 1640 medium (CELLMAX; JYC11875500BT) containing 10% fetal bovine serum (Gibco; 10091130) at 37 °C, 5% CO 2 , with 1% streptomycin and 1% penicillin as necessary.

Techniques: Infection, Cell Culture, Confocal Microscopy, Two Tailed Test, MANN-WHITNEY

Journal: iScience

Article Title: RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion

doi: 10.1016/j.isci.2026.116333

Figure Lengend Snippet: RstAB activates the expression and production of type 1 fimbria (A) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT, Δ rstAB , or Δ rstAB + P rstAB statically cultured in LB medium for 12 h. (B) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB- , or Δ rstAB + P rstAB -infected 5637 cells at 1 hpi. (C) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB -, or Δ rstAB + P rstAB -infected mouse bladders at 1 hpi. (D) Quantitative analysis of FimH protein levels in WT, Δ rstAB , or Δ rstAB + P rstAB statically grown in LB medium at 37°C overnight (top). DnaK served as a loading control. Representative image (bottom) from three independent experiments. (E) HA assays of the production of type 1 fimbria in WT, Δ rstAB , Δ rstAB + P rstAB , Δ fimA-H , or Δ fimAH Δ rstAB in the presence or absence of 3% mannose. Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined by two-tailed unpaired Student’s t test (A–D) and two-way analysis of variance (E). Significance was indicated by a p value. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. no significant difference. See also and .

Article Snippet: 5637 cells (ATCC HTB-9) were purchased from American Type Culture Collection and cultured in RPMI 1640 medium (CELLMAX; JYC11875500BT) containing 10% fetal bovine serum (Gibco; 10091130) at 37 °C, 5% CO 2 , with 1% streptomycin and 1% penicillin as necessary.

Techniques: Expressing, Cell Culture, Infection, Control, Two Tailed Test

Journal: Translational Oncology

Article Title: Prognostic value and therapeutic potential of the cuproptosis-related gene LOXL2 in thyroid cancer

doi: 10.1016/j.tranon.2026.102795

Figure Lengend Snippet: Knockdown of LOXL2 inhibits cell proliferation and metastasis. (A) The knockdown efficiency of LOXL2 at the protein level was verified via WB (LOXL2: 100 kDa; GAPDH: 37 kDa) . (B) CCK-8 assay showed the cell activity of LOXL2 knockdown TPC-1 and K1 cells was significantly weaker than that of siNC group cells. (C) EdU assay showed the cell proliferation ability of LOXL2 knockdown TPC-1 and K1 cells was significantly less than siNC group cells. (D) The cells transwell assay showed that the migration and invasion cells of LOXL2 knockdown groups were significantly less than siNC groups (100 μm). Every experiment was conducted with at least three biological replications. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The TPC-1 and K1 cell lines (ATCC, Virginia, USA) were maintained in RPMI-1640 medium (Gibco; Gaithersburg, MD, USA) supplemented with 2 mM l -glutamine(Gibco), 10 % FBS (Biological Industries; Cromwell, CT, USA), and penicillin-streptomycin(Gibco), under standard culture conditions (37 °C, 5 % CO2).

Techniques: Knockdown, CCK-8 Assay, Activity Assay, EdU Assay, Transwell Assay, Migration

Journal: Translational Oncology

Article Title: Prognostic value and therapeutic potential of the cuproptosis-related gene LOXL2 in thyroid cancer

doi: 10.1016/j.tranon.2026.102795

Figure Lengend Snippet: Elesclomol inhibits cell proliferation and metastasis. (A) Detection of IC50 values of Elesclomol in TPC-1 and K1 cell lines. (B, C) Effect of different concentrations of Elesclomol on mRNA and Protein Levels of LOXL2 detected by RT-qPCR and WB (LOXL2: 100 kDa; GAPDH: 37 kDa) . (D) CCK-8 assay showed that Elesclomol inhibited the viability of TPC-1 and K1 cells. (E) EdU assay showed that Elesclomol inhibited the proliferative capacity of TPC-1 and K1 cells. (F) Transwell assay showed that Elesclomol inhibited the migration and invasion ability of TPC-1 and K1 cells. ns, No significance; ** p < 0.01; *** p < 0.001.

Article Snippet: The TPC-1 and K1 cell lines (ATCC, Virginia, USA) were maintained in RPMI-1640 medium (Gibco; Gaithersburg, MD, USA) supplemented with 2 mM l -glutamine(Gibco), 10 % FBS (Biological Industries; Cromwell, CT, USA), and penicillin-streptomycin(Gibco), under standard culture conditions (37 °C, 5 % CO2).

Techniques: Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Transwell Assay, Migration