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hi c  (Complete Genomics Inc)


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    Complete Genomics Inc hi c
    Hi C, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 2069 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c-900/DNBSEQ-T7/pm40641325-67-31-33
    Average 99 stars, based on 2069 article reviews
    hi c - by Bioz Stars, 2026-10
    99/100 stars

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    Sequencing:

    Article Title: A single-cell profile of the porcine immune repertoire: concurrent profiling of T cell receptor and B cell receptor V(D)J sequences in health
    Article Snippet: .. The libraries were finally sequenced on a MGI DNBSEQ-T7 sequencer at a depth of at least 20,000 reads per cell using paired-end 150-bp (PE150) sequencing by BestopCell (Beijing, China). ..

    Article Title: Newborn Screening of Primary Carnitine Deficiency and Prenatal Genetic Diagnosis for a Recurrence‐Risk Family
    Article Snippet: .. Sequencing was performed on the DNBSEQ‐T7 platform (MGI, China) with an average sequencing depth ≥ 200×, and 20× coverage of the target regions reached ≥ 95%. ..

    Article Title: Accelerated hippocampal atrophy in APOE ε4 homozygotes and females: Longitudinal findings from the Tohoku Medical Megabank MRI study.
    Article Snippet: .. Multiple sequencing platforms were used, including Illumina HiSeq X Five, NovaSeq 6000, MGI DNBSeq G400, and DNBSeq T7. ..

    Article Title: Genome-wide mapping of the Galleria mellonella transcription start sites during fungal infection and treatment
    Article Snippet: .. Resulting RNA library was QCed using Agilent Bioanalyzer with High Sensitivity DNA assay kit (Agilent Technologies) and sequenced with 100 bp PE reads using a DNBSEQ G400 Sequencing platform (MGI Tech) and a DNBSEQ-T7 Sequencing platform (MGI Tech). ..

    Article Title: Photoaging-driven amplification of polystyrene microplastic toxicity in wheat: physiological and multi-omics insights
    Article Snippet: .. Library quality was assessed by electrophoresis, PicoGreen quantification, and qPCR, followed by paired-end 150 bp sequencing on Illumina NovaSeq X Plus or BGI DNBSEQ-T7 platforms. ..

    Sensitivity:

    Article Title: Genome-wide mapping of the Galleria mellonella transcription start sites during fungal infection and treatment
    Article Snippet: .. Resulting RNA library was QCed using Agilent Bioanalyzer with High Sensitivity DNA assay kit (Agilent Technologies) and sequenced with 100 bp PE reads using a DNBSEQ G400 Sequencing platform (MGI Tech) and a DNBSEQ-T7 Sequencing platform (MGI Tech). ..

    Electrophoresis:

    Article Title: Photoaging-driven amplification of polystyrene microplastic toxicity in wheat: physiological and multi-omics insights
    Article Snippet: .. Library quality was assessed by electrophoresis, PicoGreen quantification, and qPCR, followed by paired-end 150 bp sequencing on Illumina NovaSeq X Plus or BGI DNBSEQ-T7 platforms. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Photoaging-driven amplification of polystyrene microplastic toxicity in wheat: physiological and multi-omics insights
    Article Snippet: .. Library quality was assessed by electrophoresis, PicoGreen quantification, and qPCR, followed by paired-end 150 bp sequencing on Illumina NovaSeq X Plus or BGI DNBSEQ-T7 platforms. ..



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    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
    Mgi Dnbelab C Taim4, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
    Dnbelab C Series Highthroughput Single Cell Rna Library Preparation Set, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    shp  (Mini-Circuits)
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    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
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    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
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    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
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    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
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    94/100 stars
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    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
    Wild Type C Jadinii Strain Nbrc0988, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rlp  (Mini-Circuits)
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    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
    Rlp, supplied by Mini-Circuits, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    genome survey genome assembly hi c 1d library bgi dnbseq t7 clean  (Complete Genomics Inc)
    99
    Complete Genomics Inc genome survey genome assembly hi c 1d library bgi dnbseq t7 clean
    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
    Genome Survey Genome Assembly Hi C 1d Library Bgi Dnbseq T7 Clean, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c-900/DNBSEQ-T7/pm40664694-96-0-7
    Average 99 stars, based on 1 article reviews
    genome survey genome assembly hi c 1d library bgi dnbseq t7 clean - by Bioz Stars, 2026-10
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    hi c  (Complete Genomics Inc)
    99
    Complete Genomics Inc hi c
    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization <t>index</t> quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.
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    Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization index quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.

    Journal: Alzheimer's & Dementia

    Article Title: GLP‐1R agonist semaglutide protects human forebrain organoids from cholesterol‐induced neurotoxicity

    doi: 10.1002/alz.71888

    Figure Lengend Snippet: Semaglutide exerts limited neuroprotective effects in Day 80 forebrain organoids. A–B, Morphological evaluation of late‐stage forebrain organoids. Organoids were treated for 15 days (from Day 65 to Day 80) and analyzed on Day 80. A, Representative bright‐field images. Scale bar, 1 mm. B, Quantification of organoid area on Day 80. n = 11 organoids per group. C–E, Western blot analysis of AD‐associated pathological proteins. Representative bands show the expression of APP (≈ 110 kDa), p‐tau217 (≈ 55 kDa), and total tau (≈ 55 kDa), with β‐actin (42 kDa) as a loading control. D–E, Quantitative analysis of (D) relative APP levels and (E) p‐tau217/tau ratio. n = 4 independent experiments. F–H, Representative immunofluorescence images of MAP2 (green) and DAPI (blue) in Day 80 organoids. Middle panels show the MAP2 channel only, and lower panels show high‐magnification views of the boxed regions. Scale bar, 50 µm. G, Quantification of the ratio of MAP2‐positive area to DAPI‐positive area. n = 6 independent experiments. H, Quantification of MAP2 + fragmented branch density, calculated as the number of MAP2 + fragmented branches normalized to MAP2 + area. n = 6 independent experiments. I–M, Calcium imaging analysis of neuronal network activity in Day 80 organoids. I, Schematic of calcium imaging workflow and representative Fluo‐4 AM raw data. Scale bar, 50 µm. J, Representative heatmaps showing calcium transients (ΔF/F 0 ) in individual ROIs ( n = 40 cells per organoid) over a 5‐minute recording period. K–M, Quantitative analysis of (K) spontaneous calcium firing frequency (events/cell), (L) synchronization index quantified by the mean Pearson correlation coefficient of Δ F / F 0 traces across all cell pairs within each organoid, and (M) percentage of active versus inactive cells. n = 120 ROIs from n = 3 organoids across three independent differentiations. Statistical comparisons were performed based on the biological replicates ( n = 3) rather than technical ROIs. All data are presented as mean ± standard error of the mean. Statistical significance was determined using one‐way analysis of variance. ns, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. AD, Alzheimer's disease; APP, amyloid precursor protein; CHOL, cholesterol treatment; CHOL+SEMA, cholesterol + semaglutide treatment; CTR, control group; DAPI, 4′,6‐diamidino‐2‐phenylindole; MAP2, microtubule associated protein 2; p‐tau, phosphorylated tau; ROI, region of interest.

    Article Snippet: High‐throughput single‐cell encapsulation, library preparation, and paired‐end sequencing were performed on the MGI DNBelab C‐TaiM4 and DNBSEQ‐T7 platforms (BGI/Innomics).

    Techniques: Organoids, Western Blot, Analysis, Expressing, Control, Immunofluorescence, Imaging, Activity Assay