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Promega bluescript sk1
Bluescript Sk1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bluescript/bluescript+vector/pm08530411-39-8-10
Average 90 stars, based on 1 article reviews
bluescript sk1 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Synthesized:

Article Title: Cardiomyocyte Derived miR-328 Promotes Cardiac Fibrosis by Paracrinely Regulating Adjacent Fibroblasts.
Article Snippet: .. A fragment containing 6 antimiR-328 antisense units (Shanghai Biological Engineering Inc., Shanghai, China) was synthesized and subsequently subcloned into the Bluescript vector (Promega Co. Ltd., Madison, Wisconsin, USA) carrying the cardiac-specific MHC promoter. ..

Plasmid Preparation:

Article Title: Cardiomyocyte Derived miR-328 Promotes Cardiac Fibrosis by Paracrinely Regulating Adjacent Fibroblasts.
Article Snippet: .. A fragment containing 6 antimiR-328 antisense units (Shanghai Biological Engineering Inc., Shanghai, China) was synthesized and subsequently subcloned into the Bluescript vector (Promega Co. Ltd., Madison, Wisconsin, USA) carrying the cardiac-specific MHC promoter. ..

Article Title: A Novel Mechanism of Alternative Promoter and Splicing Regulates the Epitope Generation of Tumor Antigen CML66-L
Article Snippet: Specific PCR products were subcloned into the pCR-TOPO vector kit (Invitrogen, Carlsbad, CA) and sequenced at Lone Star. .. In vitro transcription and translation (TNT) Bluescript plasmid containing cDNA encoding CML66-L, plasmid pTriplEx containing cDNA encoding CML66-S, and plasmid pTriplEx without cDNA insert (as a negative control) were used (1 μg each) in the in vitro TNT, which were then performed using a TNT T7/T3-coupled system according to the manufacturer’s protocol (Promega, Madison, WI) ( 12 ). ..

Article Title: Method of extracting chromatin fractions from intact cells
Article Snippet: .. BlueScript plasmid (6.5 μg) was relaxed with 15 IU of Topoisomerase I (Topo I, Promega) in PBS/1 mM EDTA. ..

Article Title: Cardiomyocyte Derived miR-328 Promotes Cardiac Fibrosis by Paracrinely Regulating Adjacent Fibroblasts.
Article Snippet: .. A fragment containing the pre-miR-328 sequence was subcloned into the Bluescript vector (Promega Co. Ltd., Madison, Wisconsin, USA) carrying the cardiac-specific α-myosin heavy chain (αMHC) promoter. ..

Article Title: Overexpression of microRNA-1 Causes Atrioventricular Block in Rodents
Article Snippet: .. The fragment was then subcloned into the SalI and HindIII sites of the Bluescript vector (Promega, Madison, WI) carrying the cardiac-specific α-myosin heavy chain (α-MHC) promoter and human growth hormone poly(A) + signal. ..

Immunopeptidomics:

Article Title: Cardiomyocyte Derived miR-328 Promotes Cardiac Fibrosis by Paracrinely Regulating Adjacent Fibroblasts.
Article Snippet: .. A fragment containing 6 antimiR-328 antisense units (Shanghai Biological Engineering Inc., Shanghai, China) was synthesized and subsequently subcloned into the Bluescript vector (Promega Co. Ltd., Madison, Wisconsin, USA) carrying the cardiac-specific MHC promoter. ..

Produced:

Article Title: Expression of apoAI and variants thereof using spliceosome mediated RNA trans-splicing
Article Snippet: .. RNAs may be produced in high yield via in vitro transcription using plasmids such as SPS65 and Bluescript (Promega Corporation, Madison, Wis.). ..

Article Title: Correction of alpha-1-antitrypsin genetic defects using spliceosome mediated RNA trans splicing
Article Snippet: .. RNAs may be produced in high yield via in vitro transcription using plasmids such as SPS65 and Bluescript (Promega Corporation, Madison, Wis.). ..

In Vitro:

Article Title: Expression of apoAI and variants thereof using spliceosome mediated RNA trans-splicing
Article Snippet: .. RNAs may be produced in high yield via in vitro transcription using plasmids such as SPS65 and Bluescript (Promega Corporation, Madison, Wis.). ..

Article Title: A Novel Mechanism of Alternative Promoter and Splicing Regulates the Epitope Generation of Tumor Antigen CML66-L
Article Snippet: Specific PCR products were subcloned into the pCR-TOPO vector kit (Invitrogen, Carlsbad, CA) and sequenced at Lone Star. .. In vitro transcription and translation (TNT) Bluescript plasmid containing cDNA encoding CML66-L, plasmid pTriplEx containing cDNA encoding CML66-S, and plasmid pTriplEx without cDNA insert (as a negative control) were used (1 μg each) in the in vitro TNT, which were then performed using a TNT T7/T3-coupled system according to the manufacturer’s protocol (Promega, Madison, WI) ( 12 ). ..

Article Title: Correction of alpha-1-antitrypsin genetic defects using spliceosome mediated RNA trans splicing
Article Snippet: .. RNAs may be produced in high yield via in vitro transcription using plasmids such as SPS65 and Bluescript (Promega Corporation, Madison, Wis.). ..

Negative Control:

Article Title: A Novel Mechanism of Alternative Promoter and Splicing Regulates the Epitope Generation of Tumor Antigen CML66-L
Article Snippet: Specific PCR products were subcloned into the pCR-TOPO vector kit (Invitrogen, Carlsbad, CA) and sequenced at Lone Star. .. In vitro transcription and translation (TNT) Bluescript plasmid containing cDNA encoding CML66-L, plasmid pTriplEx containing cDNA encoding CML66-S, and plasmid pTriplEx without cDNA insert (as a negative control) were used (1 μg each) in the in vitro TNT, which were then performed using a TNT T7/T3-coupled system according to the manufacturer’s protocol (Promega, Madison, WI) ( 12 ). ..

Sequencing:

Article Title: Cardiomyocyte Derived miR-328 Promotes Cardiac Fibrosis by Paracrinely Regulating Adjacent Fibroblasts.
Article Snippet: .. A fragment containing the pre-miR-328 sequence was subcloned into the Bluescript vector (Promega Co. Ltd., Madison, Wisconsin, USA) carrying the cardiac-specific α-myosin heavy chain (αMHC) promoter. ..

other:

Article Title: At Least Two Genes Encode Many Variants of Irak3 in Rainbow Trout, but Neither the Full-Length Factor Nor Its Variants Interfere Directly With the TLR-Mediated Stimulation of Inflammation
Article Snippet: The retrieved fragments were finally inserted “in frame” into our expression vector 280, a modified ( ) CMV-driven pKS(+) Bluescript plasmid (Promega).



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