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hcn4  (Alomone Labs)


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    Alomone Labs hcn4
    Relative expression levels of hcn1 , hcn2a , hcn2b and <t>hcn4</t> in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.
    Hcn4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blp-pc056/pmc08307021-170-9-29?v=Alomone+Labs
    Average 90 stars, based on 1 article reviews
    hcn4 - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)"

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22147539

    Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.
    Figure Legend Snippet: Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.

    Techniques Used: Expressing

    Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and HCN4 (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.
    Figure Legend Snippet: Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and HCN4 (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.

    Techniques Used: Immunolabeling

    Primary and secondary antibodies used in the present study.
    Figure Legend Snippet: Primary and secondary antibodies used in the present study.

    Techniques Used:

    Details of the primers used for relative quantification of mRNA levels by qPCR. In addition to the primer sequences used to quantify each transcript level, amplicon sizes, annealing temperatures (Ta) and amplification efficiencies (E) are indicated.
    Figure Legend Snippet: Details of the primers used for relative quantification of mRNA levels by qPCR. In addition to the primer sequences used to quantify each transcript level, amplicon sizes, annealing temperatures (Ta) and amplification efficiencies (E) are indicated.

    Techniques Used: Quantitative Proteomics, Amplification, Sequencing



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    Relative expression levels of <t>hcn1</t> , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.
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    Relative expression levels of hcn1 , hcn2a , hcn2b and <t>hcn4</t> in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.
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    Relative expression levels of hcn1 , hcn2a , hcn2b and <t>hcn4</t> in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.
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    Image Search Results


    Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.

    Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

    Techniques: Expressing

    Multiple clusters of pacemaker cells detected by the double immunolabeling with antibodies against Islet-1 (green signal), and HCN1 and HCN2 (red signal) in the SAR. ( a ) Pacemaker cells (arrows) embedded in the myocardium (M) expressing Islet-1 (green signal) and HCN1 (red signal). ( b – f ) Pacemaker cells revealed colocalization of Islet-1 and HCN2 ( d ; merge). Two populations expressing Islet-1 (green signal and arrows) and HCN2 (red signal and arrows), respectively, were observed in e and f. Scale bars: 20 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Multiple clusters of pacemaker cells detected by the double immunolabeling with antibodies against Islet-1 (green signal), and HCN1 and HCN2 (red signal) in the SAR. ( a ) Pacemaker cells (arrows) embedded in the myocardium (M) expressing Islet-1 (green signal) and HCN1 (red signal). ( b – f ) Pacemaker cells revealed colocalization of Islet-1 and HCN2 ( d ; merge). Two populations expressing Islet-1 (green signal and arrows) and HCN2 (red signal and arrows), respectively, were observed in e and f. Scale bars: 20 µm.

    Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

    Techniques: Immunolabeling, Expressing

    Primary and secondary antibodies used in the present study.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Primary and secondary antibodies used in the present study.

    Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

    Techniques:

    Details of the primers used for relative quantification of mRNA levels by qPCR. In addition to the primer sequences used to quantify each transcript level, amplicon sizes, annealing temperatures (Ta) and amplification efficiencies (E) are indicated.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Details of the primers used for relative quantification of mRNA levels by qPCR. In addition to the primer sequences used to quantify each transcript level, amplicon sizes, annealing temperatures (Ta) and amplification efficiencies (E) are indicated.

    Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

    Techniques: Quantitative Proteomics, Amplification, Sequencing

    Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Relative expression levels of hcn1 , hcn2a , hcn2b and hcn4 in sinus, atrium, ventricle and bulbus arteriosus in Atlantic cod. Transcripts were quantified by qPCR, normalized using the geometric average of ubi and eef1 expression and shown as relative values compared to hcn1 transcript levels in each sample. Data are expressed in arbitrary units (A.U.) as mean ± S.E. ( n = 5). Different superscript letters ( a, b ) indicate significant differences in transcript levels between hcn paralogues in each heart region. Differences in hcn transcript levels within each heart area were determined by one-way ANOVA with a Holm–Sidak post hoc test ( p < 0.05). Kruskal–Wallis one-way ANOVA on ranks with Dunn’s post hoc test was performed when the data did not meet the normality and equal variance requirements.

    Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

    Techniques: Expressing

    Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and HCN4 (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Double immunolabeling with antibodies against Hu (( a ), green signal and arrows) and HCN4 (( b ), red signal and arrows) in the SAR. HCN4 was weakly expressed in the pacemaker cells (green, red, and white arrows) that appeared to be innervated by Hu-immunoreactive axons and nerve fibers (arrowheads). Areas of pacemaker tissue with abundant pacemaker cells are boxed in ( c ). Scale bars: 20 µm.

    Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

    Techniques: Immunolabeling

    Primary and secondary antibodies used in the present study.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Primary and secondary antibodies used in the present study.

    Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

    Techniques:

    Details of the primers used for relative quantification of mRNA levels by qPCR. In addition to the primer sequences used to quantify each transcript level, amplicon sizes, annealing temperatures (Ta) and amplification efficiencies (E) are indicated.

    Journal: International Journal of Molecular Sciences

    Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)

    doi: 10.3390/ijms22147539

    Figure Lengend Snippet: Details of the primers used for relative quantification of mRNA levels by qPCR. In addition to the primer sequences used to quantify each transcript level, amplicon sizes, annealing temperatures (Ta) and amplification efficiencies (E) are indicated.

    Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.

    Techniques: Quantitative Proteomics, Amplification, Sequencing