Journal: International Journal of Molecular Sciences
Article Title: Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)
doi: 10.3390/ijms22147539
Figure Lengend Snippet: Multiple clusters of pacemaker cells detected by the double immunolabeling with antibodies against Islet-1 (green signal), and HCN1 and HCN2 (red signal) in the SAR. ( a ) Pacemaker cells (arrows) embedded in the myocardium (M) expressing Islet-1 (green signal) and HCN1 (red signal). ( b – f ) Pacemaker cells revealed colocalization of Islet-1 and HCN2 ( d ; merge). Two populations expressing Islet-1 (green signal and arrows) and HCN2 (red signal and arrows), respectively, were observed in e and f. Scale bars: 20 µm.
Article Snippet: Pre-absorption of the primary antisera to HCN1, HCN2 and HCN4 with the respective blocking peptide (HCN1 blocking peptide, BLP-PC056, HCN2 blocking peptide, BLP-P030 and HCN4 blocking peptide, BLP-PCO52 from Alomone Labs) according to the guidelines of the supplier led to the complete elimination of the immunostaining of the pacemaker tissue.
Techniques: Immunolabeling, Expressing