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anti-5ht7 receptor/htr7 (extracellular) antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti-5ht7 receptor/htr7 (extracellular) antibody
    Anti 5ht7 Receptor/Htr7 (Extracellular) Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/asr-037/Anti-5HT7+Receptor%2FHTR7+(extracellular)+Antibody/custom%40asr-037%4038215751
    Average 89 stars, based on 1 article reviews
    anti-5ht7 receptor/htr7 (extracellular) antibody - by Bioz Stars, 2026-09
    89/100 stars

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    Related Articles

    Incubation:

    Article Title: Serotonin receptor 5-HT7 modulates inflammatory-associated functions of macrophages
    Article Snippet: Page 6/26 Immuno uorescence staining and imaging THP-1-derived macrophages were grown on glass cover slips and xed using paraformaldehyde (PFA, Carl Roth, 0335.3, 4%) for 10 min at RT, permeabilized with acetone for 3 min at -20°C, washed with PBS and unspeci c binding sites were blocked with albumin fraction V (Carl Roth, T844.4, %) in PBS for 1 h at room temperature. .. Afterwards, cells were incubated with anti-5HT7 Receptor/HTR7 (extracellular)-FITC antibody (1:400 in PBS, ASR-037-F, Alomone Labs) or 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (1:10 ratio to the antibody according to the manufacturers recommendations; BLP-SR037, Alomone Labs) for 1 h at room temperature. .. Nuclei were stained using DAPI (1:5000, Sigma Aldrich, D9542) for 5 min at RT.

    Article Title: Serotonin receptor 5-HT7 modulates inflammatory-associated functions of macrophages
    Article Snippet: THP-1-derived macrophages were grown on glass cover slips and fixed using paraformaldehyde (PFA, Carl Roth, 0335.3, 4%) for 10 min at RT, permeabilized with acetone for 3 min at -20 °C, washed with PBS and unspecific binding sites were blocked with albumin fraction V (Carl Roth, T844.4, 1%) in PBS for 1 h at RT. .. Afterwards, cells were incubated with anti-5HT7 Receptor/HTR7 (extracellular)-FITC antibody (1:400 in PBS, Alomone Labs, ASR-037-F) or 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (1:10 ratio to the antibody according to the manufacturers recommendations; Alomone Labs, BLP-SR037) for 1 h at RT. .. Nuclei were stained using DAPI (1:5000, Sigma Aldrich, D9542) for 5 min at RT.

    Blocking Assay:

    Article Title: Serotonin receptor 5-HT7 modulates inflammatory-associated functions of macrophages
    Article Snippet: Page 6/26 Immuno uorescence staining and imaging THP-1-derived macrophages were grown on glass cover slips and xed using paraformaldehyde (PFA, Carl Roth, 0335.3, 4%) for 10 min at RT, permeabilized with acetone for 3 min at -20°C, washed with PBS and unspeci c binding sites were blocked with albumin fraction V (Carl Roth, T844.4, %) in PBS for 1 h at room temperature. .. Afterwards, cells were incubated with anti-5HT7 Receptor/HTR7 (extracellular)-FITC antibody (1:400 in PBS, ASR-037-F, Alomone Labs) or 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (1:10 ratio to the antibody according to the manufacturers recommendations; BLP-SR037, Alomone Labs) for 1 h at room temperature. .. Nuclei were stained using DAPI (1:5000, Sigma Aldrich, D9542) for 5 min at RT.

    Article Title: Serotonin receptor 5-HT7 modulates inflammatory-associated functions of macrophages
    Article Snippet: THP-1-derived macrophages were grown on glass cover slips and fixed using paraformaldehyde (PFA, Carl Roth, 0335.3, 4%) for 10 min at RT, permeabilized with acetone for 3 min at -20 °C, washed with PBS and unspecific binding sites were blocked with albumin fraction V (Carl Roth, T844.4, 1%) in PBS for 1 h at RT. .. Afterwards, cells were incubated with anti-5HT7 Receptor/HTR7 (extracellular)-FITC antibody (1:400 in PBS, Alomone Labs, ASR-037-F) or 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (1:10 ratio to the antibody according to the manufacturers recommendations; Alomone Labs, BLP-SR037) for 1 h at RT. .. Nuclei were stained using DAPI (1:5000, Sigma Aldrich, D9542) for 5 min at RT.



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