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solution alomone labs asr 033  (Alomone Labs)


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    Alomone Labs solution alomone labs asr 033
    Solution Alomone Labs Asr 033, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/asr-033/Anti-5HT2A+Receptor+Antibody/ppr0951182-509-97-98
    Average 93 stars, based on 3 article reviews
    solution alomone labs asr 033 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Expression of serotonin transporter (SERT) and receptors 5-HTR1A and 5-HTR2A in an animal model of hypothermia.
    Article Snippet: For qRT-PCR total RNA was extracted from fresh samples with a GenEluteTM kit (Sigma), followed by RT and qPCR with hydrolysis probes (Applied Biosystems). .. For immunofluorescence 200 μm slices were cut from fixed brains and stained with primary antibodies ASR-021, ASR-033, AMT-004 (Alomone Labs) and secondary antibody N2404-Ab635P-L (NanoTag), co-stained with phalloidin-AlexaFluor488 and DAPI. ..

    Staining:

    Article Title: Expression of serotonin transporter (SERT) and receptors 5-HTR1A and 5-HTR2A in an animal model of hypothermia.
    Article Snippet: For qRT-PCR total RNA was extracted from fresh samples with a GenEluteTM kit (Sigma), followed by RT and qPCR with hydrolysis probes (Applied Biosystems). .. For immunofluorescence 200 μm slices were cut from fixed brains and stained with primary antibodies ASR-021, ASR-033, AMT-004 (Alomone Labs) and secondary antibody N2404-Ab635P-L (NanoTag), co-stained with phalloidin-AlexaFluor488 and DAPI. ..

    other:

    Article Title: Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion
    Article Snippet: Cy3 AffiniPure donkey anti-rabbit IgG (Jackson ImmunoResearch, 711-165-152, lot: 141941) 15. rabbit anti-5-HT2A receptor antibody (Alomone, ASR-033) 16. rabbit anti-5-HT2A receptor antibody (Immunostar, 24288, lot: 2041001L) 1. https://www.thermofisher.com/antibody/product/GFP-Antibody-Polyclonal/A-11122 2. https://www.merckmillipore.com/GB/en/product/Anti-Tryptophan-Hydroxylase-Antibody,MM_NF-AB1541 3. https://nittobo-nmd.co.jp/pdf/reagents/HTT.pdf 4. https://www.novusbio.com/products/c-fos-antibody-2h2_nbp2-50037 5. https://labchem-wako.fujifilm.com/europe/product/detail/W01W0101-2254.html 6.

    Blocking Assay:

    Article Title: Psilocin fosters neuroplasticity in iPSC-derived human cortical neurons
    Article Snippet: .. Antibody (clone) Host species Dilution Blocking Solution in 10% Fetal bovine serum (FBS) Manufacturer Catalogu e number Registered office BDNF rabbit 1:300 0.1% Saponin Elabscience E-AB18244.20 0 Houston, USA CTIP-2 (25B6) rat 1:500 0.3% Triton X Abcam ab18465 Cambridge, UK cFOS (9F6) rabbit 1:500 0.3% Triton X Cell Signaling Technologies 2250 Danvers, USA FOXA2 goat 1:500 0.3% Triton X R&D Systems AF2400 Minneapolis , USA OCT3/4 (A-9) mouse 1:500 0.3% Triton X Santa Cruz Sc365509 Santa Cruz, USA GFAP mouse 1:500 0.1% Triton X Synaptic Systems 173011 Göttingen, Germany Extracellular epitode of 5- HT2A-R rabbit 1:100 Blocking solution Alomone Labs ASR-033 Jerusalem, Israel MAP2 chicken 1:7000 0.1% Saponin/ 0.1% - 0.3% Triton X BioLegend 822501 San Diego, USA mCherry rabbit 1:1000 0.3% Triton X Cell Signaling Technologies 43590S Danvers, USA NESTIN 196908 mouse 1:600 0.3% Triton X R&D Systems MAB1259 Minneapolis , USA NeuN (D4G4O) rabbit 1:200 0.3% Triton X Cell Signaling Technologies 24307S Danvers, USA PAX6 (Poly19013) rabbit 1:500 0.3% Triton X Biolegend 901301 San Diego, USA PSD-95 rabbit 1:250 0.1% Saponin Cell Signaling Technologies 2507S Danvers, USA SOX2 (D6D9) rabbit 1:500 0.3% Triton X Cell Signaling Technologies 3579S Danvers, USA Synapsin I/II/III (A17080A) mouse 1:500 0.1% Saponin BioLegend 853701 San Diego, USA TAU guinea-pig 1:700 0.1% Saponin/ 0.1% - 0.3% Triton X Synaptic Systems 314004 Göttingen, Germany TBR1 rabbit 1:500 0.3% Triton X Abcam ab31940 Cambridge, UK vGLUT2 guinea-pig 1:200 0.1% Triton X Synaptic Systems 135304 Göttingen, Germany Table S2: Secondary antibodies used for ICC. .. Antibody Host species Manufacturer Catalogue number Registered office anti-chicken IgG Alexa Fluor-488 goat Thermo Fisher Scientific A11039 Waltham, USA anti-guinea-pig IgG Alexa Fluor488 goat Thermo Fisher Scientific A11073 Waltham, USA anti-guinea-pig IgG Alexa Fluor568 goat Thermo Fisher Scientific A11075 Waltham, USA anti-mouse IgG Alexa Fluor-488 goat Thermo Fisher Scientific A11001 Waltham, USA anti-mouse IgG Alexa Fluor-568 goat Thermo Fisher Scientific A11004 Waltham, USA anti-rabbit IgG Alexa Fluor-488 goat Thermo Fisher Scientific A11008 Waltham, USA anti-rabbit IgG Alexa Fluor-555 goat Thermo Fisher Scientific A21428 Waltham, USA anti-rabbit IgG Alexa Fluor-647 goat Thermo Fisher Scientific A21244 Waltham, USA anti-rat IgG Alexa Fluor-555 goat Thermo Fisher Scientific A21434 Waltham, USA

    Article Title: Investigation of neuromodulation of the endbulb of Held synapse in the cochlear nucleus by serotonin and norepinephrine
    Article Snippet: Rabbit-anti-5-HT 1B receptor + blocking peptide , Alomone Labs, Israel. .. Rabbit-anti-5-HT 2A receptor + blocking peptide , Alomone Labs, Israel. .. Rabbit-anti-5-HT 2B receptor + blocking peptide , Alomone Labs, Israel.

    Immunocytochemistry:

    Article Title: Psilocin fosters neuroplasticity in iPSC-derived human cortical neurons
    Article Snippet: .. Antibody (clone) Host species Dilution Blocking Solution in 10% Fetal bovine serum (FBS) Manufacturer Catalogu e number Registered office BDNF rabbit 1:300 0.1% Saponin Elabscience E-AB18244.20 0 Houston, USA CTIP-2 (25B6) rat 1:500 0.3% Triton X Abcam ab18465 Cambridge, UK cFOS (9F6) rabbit 1:500 0.3% Triton X Cell Signaling Technologies 2250 Danvers, USA FOXA2 goat 1:500 0.3% Triton X R&D Systems AF2400 Minneapolis , USA OCT3/4 (A-9) mouse 1:500 0.3% Triton X Santa Cruz Sc365509 Santa Cruz, USA GFAP mouse 1:500 0.1% Triton X Synaptic Systems 173011 Göttingen, Germany Extracellular epitode of 5- HT2A-R rabbit 1:100 Blocking solution Alomone Labs ASR-033 Jerusalem, Israel MAP2 chicken 1:7000 0.1% Saponin/ 0.1% - 0.3% Triton X BioLegend 822501 San Diego, USA mCherry rabbit 1:1000 0.3% Triton X Cell Signaling Technologies 43590S Danvers, USA NESTIN 196908 mouse 1:600 0.3% Triton X R&D Systems MAB1259 Minneapolis , USA NeuN (D4G4O) rabbit 1:200 0.3% Triton X Cell Signaling Technologies 24307S Danvers, USA PAX6 (Poly19013) rabbit 1:500 0.3% Triton X Biolegend 901301 San Diego, USA PSD-95 rabbit 1:250 0.1% Saponin Cell Signaling Technologies 2507S Danvers, USA SOX2 (D6D9) rabbit 1:500 0.3% Triton X Cell Signaling Technologies 3579S Danvers, USA Synapsin I/II/III (A17080A) mouse 1:500 0.1% Saponin BioLegend 853701 San Diego, USA TAU guinea-pig 1:700 0.1% Saponin/ 0.1% - 0.3% Triton X Synaptic Systems 314004 Göttingen, Germany TBR1 rabbit 1:500 0.3% Triton X Abcam ab31940 Cambridge, UK vGLUT2 guinea-pig 1:200 0.1% Triton X Synaptic Systems 135304 Göttingen, Germany Table S2: Secondary antibodies used for ICC. .. Antibody Host species Manufacturer Catalogue number Registered office anti-chicken IgG Alexa Fluor-488 goat Thermo Fisher Scientific A11039 Waltham, USA anti-guinea-pig IgG Alexa Fluor488 goat Thermo Fisher Scientific A11073 Waltham, USA anti-guinea-pig IgG Alexa Fluor568 goat Thermo Fisher Scientific A11075 Waltham, USA anti-mouse IgG Alexa Fluor-488 goat Thermo Fisher Scientific A11001 Waltham, USA anti-mouse IgG Alexa Fluor-568 goat Thermo Fisher Scientific A11004 Waltham, USA anti-rabbit IgG Alexa Fluor-488 goat Thermo Fisher Scientific A11008 Waltham, USA anti-rabbit IgG Alexa Fluor-555 goat Thermo Fisher Scientific A21428 Waltham, USA anti-rabbit IgG Alexa Fluor-647 goat Thermo Fisher Scientific A21244 Waltham, USA anti-rat IgG Alexa Fluor-555 goat Thermo Fisher Scientific A21434 Waltham, USA

    Immunohistochemistry:

    Article Title: Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion
    Article Snippet: The brains were frozen and cryosectioned into 30 μm-thick coronal sections with the cryostat (Leica CM3050S; Leica Biosystems) and stored at −80 °C until immunohistochemical processing. .. For immunohistochemistry, the sections were immersed in 0.25% Triton X-100 (Nacalai Tesque) in PBS (PBS-T) for permeabilization and then incubated overnight at 4 °C with rabbit polyclonal anti-green fluorescent protein (GFP) antibody (1:2000; A-11122, Thermo Fisher Scientific), sheep polyclonal anti-tryptophan hydroxylase (Tph) antibody (1:200; AB1541, Merck Millipore), goat polyclonal anti-SERT antibody (1:200; HTT-Go-Af970, Nittobo Medical), mouse monoclonal anti-c-Fos antibody (2H2) (1:200; NBP2-50037, Novus Biologicals), rabbit polyclonal anti-5-HT 2A receptor antibody (1:500; ASR-033, Alomone), or rabbit polyclonal anti-5-HT 2A receptor antibody (1:100; #24288, ImmunoStar), followed by incubation with Alexa Fluor 594-labeled donkey anti-rabbit IgG (1:200; A-21207, Thermo Fisher Scientific), Alexa Fluor 647-labeled donkey anti-sheep IgG (1:200; A-21448, Thermo Fisher Scientific), Alexa Fluor 488-labeled donkey anti-rabbit IgG (1:200; A-21206, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-goat IgG (1:200; A-11058, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-sheep IgG (1:200; A-11016, Thermo Fisher Scientific), or Alexa Fluor 647-labeled donkey anti-mouse IgG (1:200; A-31571, Thermo Fisher Scientific) for 2 h at room temperature. ..

    Article Title: Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion.
    Article Snippet: The brains were frozen and cryosectioned into 30μm-thick coronal sections with the cryostat (Leica CM3050S; Leica Biosystems) and stored at −80 °C until immunohistochemical processing. .. For immunohistochemistry, the sections were immersed in 0.25% Triton X-100 (Nacalai Tesque) in PBS (PBS-T) for permeabilization and then incubated overnight at 4 °C with rabbit polyclonal antigreen fluorescent protein (GFP) antibody (1:2000; A-11122, Thermo Fisher Scientific), sheep polyclonal anti-tryptophan hydroxylase (Tph) antibody (1:200; AB1541, Merck Millipore), goat polyclonal anti-SERT antibody (1:200; HTT-Go-Af970, NittoboMedical), mouse monoclonal anti-c-Fos antibody (2H2) (1:200; NBP2-50037, Novus Biologicals), rabbit polyclonal anti-5-HT2A receptor antibody (1:500; ASR-033, Alomone), or rabbit polyclonal anti-5-HT2A receptor antibody (1:100; #24288, ImmunoStar), followed by incubation with Alexa Fluor 594- labeled donkey anti-rabbit IgG (1:200; A-21207, Thermo Fisher Scientific), Alexa Fluor 647-labeled donkey anti-sheep IgG (1:200; A-21448, Thermo Fisher Scientific), Alexa Fluor 488-labeled donkey anti-rabbit IgG (1:200; A-21206, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-goat IgG (1:200; A-11058, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-sheep IgG (1:200; A-11016, Thermo Fisher Scientific), or Alexa Fluor 647-labeled donkey anti-mouse IgG (1:200; A-31571, Thermo Fisher Scientific) for 2 h at room temperature. ..

    Incubation:

    Article Title: Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion
    Article Snippet: The brains were frozen and cryosectioned into 30 μm-thick coronal sections with the cryostat (Leica CM3050S; Leica Biosystems) and stored at −80 °C until immunohistochemical processing. .. For immunohistochemistry, the sections were immersed in 0.25% Triton X-100 (Nacalai Tesque) in PBS (PBS-T) for permeabilization and then incubated overnight at 4 °C with rabbit polyclonal anti-green fluorescent protein (GFP) antibody (1:2000; A-11122, Thermo Fisher Scientific), sheep polyclonal anti-tryptophan hydroxylase (Tph) antibody (1:200; AB1541, Merck Millipore), goat polyclonal anti-SERT antibody (1:200; HTT-Go-Af970, Nittobo Medical), mouse monoclonal anti-c-Fos antibody (2H2) (1:200; NBP2-50037, Novus Biologicals), rabbit polyclonal anti-5-HT 2A receptor antibody (1:500; ASR-033, Alomone), or rabbit polyclonal anti-5-HT 2A receptor antibody (1:100; #24288, ImmunoStar), followed by incubation with Alexa Fluor 594-labeled donkey anti-rabbit IgG (1:200; A-21207, Thermo Fisher Scientific), Alexa Fluor 647-labeled donkey anti-sheep IgG (1:200; A-21448, Thermo Fisher Scientific), Alexa Fluor 488-labeled donkey anti-rabbit IgG (1:200; A-21206, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-goat IgG (1:200; A-11058, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-sheep IgG (1:200; A-11016, Thermo Fisher Scientific), or Alexa Fluor 647-labeled donkey anti-mouse IgG (1:200; A-31571, Thermo Fisher Scientific) for 2 h at room temperature. ..

    Article Title: Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion.
    Article Snippet: The brains were frozen and cryosectioned into 30μm-thick coronal sections with the cryostat (Leica CM3050S; Leica Biosystems) and stored at −80 °C until immunohistochemical processing. .. For immunohistochemistry, the sections were immersed in 0.25% Triton X-100 (Nacalai Tesque) in PBS (PBS-T) for permeabilization and then incubated overnight at 4 °C with rabbit polyclonal antigreen fluorescent protein (GFP) antibody (1:2000; A-11122, Thermo Fisher Scientific), sheep polyclonal anti-tryptophan hydroxylase (Tph) antibody (1:200; AB1541, Merck Millipore), goat polyclonal anti-SERT antibody (1:200; HTT-Go-Af970, NittoboMedical), mouse monoclonal anti-c-Fos antibody (2H2) (1:200; NBP2-50037, Novus Biologicals), rabbit polyclonal anti-5-HT2A receptor antibody (1:500; ASR-033, Alomone), or rabbit polyclonal anti-5-HT2A receptor antibody (1:100; #24288, ImmunoStar), followed by incubation with Alexa Fluor 594- labeled donkey anti-rabbit IgG (1:200; A-21207, Thermo Fisher Scientific), Alexa Fluor 647-labeled donkey anti-sheep IgG (1:200; A-21448, Thermo Fisher Scientific), Alexa Fluor 488-labeled donkey anti-rabbit IgG (1:200; A-21206, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-goat IgG (1:200; A-11058, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-sheep IgG (1:200; A-11016, Thermo Fisher Scientific), or Alexa Fluor 647-labeled donkey anti-mouse IgG (1:200; A-31571, Thermo Fisher Scientific) for 2 h at room temperature. ..

    Labeling:

    Article Title: Median raphe serotonergic neurons projecting to the interpeduncular nucleus control preference and aversion.
    Article Snippet: The brains were frozen and cryosectioned into 30μm-thick coronal sections with the cryostat (Leica CM3050S; Leica Biosystems) and stored at −80 °C until immunohistochemical processing. .. For immunohistochemistry, the sections were immersed in 0.25% Triton X-100 (Nacalai Tesque) in PBS (PBS-T) for permeabilization and then incubated overnight at 4 °C with rabbit polyclonal antigreen fluorescent protein (GFP) antibody (1:2000; A-11122, Thermo Fisher Scientific), sheep polyclonal anti-tryptophan hydroxylase (Tph) antibody (1:200; AB1541, Merck Millipore), goat polyclonal anti-SERT antibody (1:200; HTT-Go-Af970, NittoboMedical), mouse monoclonal anti-c-Fos antibody (2H2) (1:200; NBP2-50037, Novus Biologicals), rabbit polyclonal anti-5-HT2A receptor antibody (1:500; ASR-033, Alomone), or rabbit polyclonal anti-5-HT2A receptor antibody (1:100; #24288, ImmunoStar), followed by incubation with Alexa Fluor 594- labeled donkey anti-rabbit IgG (1:200; A-21207, Thermo Fisher Scientific), Alexa Fluor 647-labeled donkey anti-sheep IgG (1:200; A-21448, Thermo Fisher Scientific), Alexa Fluor 488-labeled donkey anti-rabbit IgG (1:200; A-21206, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-goat IgG (1:200; A-11058, Thermo Fisher Scientific), Alexa Fluor 594-labeled donkey anti-sheep IgG (1:200; A-11016, Thermo Fisher Scientific), or Alexa Fluor 647-labeled donkey anti-mouse IgG (1:200; A-31571, Thermo Fisher Scientific) for 2 h at room temperature. ..



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