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5 ht2a receptor antibodies  (Alomone Labs)


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    Alomone Labs 5 ht2a receptor antibodies
    5 Ht2a Receptor Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/asr-033/Guinea+Pig+Anti-5HT2A+Receptor+(HTR2A)+(extracellular)+Antibody/pmc09780347__41467_2022_35346_MOESM2_ESM-266-6-9
    Average 93 stars, based on 1 article reviews
    5 ht2a receptor antibodies - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Saline:

    Article Title: Serotonin functions as a bidirectional guidance molecule regulating growth cone motility
    Article Snippet: Pharmacological agents were bath-applied to cultures 20 min prior to growth cone turning assay as listed: chlorpromazine (5 μM,Sigma-Aldrich), ritanserin (1 nM, Tocris), GR-55562 dihydrochloride (500 nM, R&D Systems), Sp-cAMPs (20 μM, BioLog Life Science), U-731222 (20 nM, Sigma-Aldrich), forskolin (5 μM, Sigma-Aldrich), nifedipine (5 μM, Alomone Labs), thapsigargin (50 nM, Alomone Labs), ondansentron (10 nM, Tocris), TCB-2 ((4-Bromo-3,6-dimethoxybenzocyclobuten-1-yl)methylamine hydrobromide, 2 nM, Tocris), CP 94253 hydrochloride (5-Propoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1H-pyrrolo[3,2-b]pyridine hydrochloride, 5 nM, Tocris). .. DRG sensory neurons were fixed with paraformaldehyde (PFA) (4%, Sigma-Aldrich) in phosphate buffered saline (PBS) overnight at 4 °C, washed in PBS (3 × 10 min), for some experiments, cells were permeabilised for 1 h with blocking solution (PBS containing 0.4% Triton X-100; 5% goat serum, Sigma-Aldrich & Gibco) and immunostained with a rabbit antibody to serotonin receptors 5-HT1b (1:500; Abcam) or 5-HT2a (1:500; Alomone Labs) in blocking solution overnight at 4 °C. .. Following washes in PBS (3 × 10 min), Alexa Fluor goat anti-rabbit 488/568/647 secondary antibodies (1:1000, Invitrogen) were incubated for 1 h and rinsed in PBS (3x10 min) prior to 4% PFA incubation overnight at 4 °C.

    Article Title: Serotonin functions as a bidirectional guidance molecule regulating growth cone motility
    Article Snippet: Pharmacological agents Pharmacological agents were bath-applied to cultures 20 min prior to growth cone turning assay as listed: chlorpromazine (5 μM,Sigma-Aldrich), ritanserin (1 nM, Tocris), GR-55562 dihydrochloride (500 nM, R&D Systems), Sp-cAMPs (20 μM, BioLog Life Science), U-731222 (20 nM, Sigma-Aldrich), forskolin (5 μM, Sigma-Aldrich), nifedipine (5 μM, Alomone Labs), thapsigargin (50 nM, Alomone Labs), ondansentron (10 nM, Tocris), TCB-2 ((4-Bromo-3,6-dimethoxybenzocyclobuten-1-yl)methylamine hydrobromide, 2 nM, Tocris), CP 94253 hydrochloride (5-Propoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1H-pyrrolo[3,2-b]pyridine hydrochloride, 5 nM, Tocris). .. Immunocytochemistry DRG sensory neurons were fixed with paraformaldehyde (PFA) (4%, Sigma-Aldrich) in phosphate buffered saline (PBS) overnight at 4 °C, washed in PBS (3 × 10 min), for some experiments, cells were permeabilised for 1 h with blocking solution (PBS containing 0.4% Triton X-100; 5% goat serum, Sigma-Aldrich & Gibco) and immunostained with a rabbit antibody to serotonin receptors 5-HT1b (1:500; Abcam) or 5-HT2a (1:500; Alomone Labs) in blocking solution overnight at 4 °C. .. Following washes in PBS (3 × 10 min), Alexa Fluor goat anti-rabbit 488/568/647 secondary antibodies (1:1000, Invitrogen) were incubated for 1 h and rinsed in PBS (3x10 min) prior to 4% PFA incubation overnight at 4 °C.

    Article Title: Serotonin functions as a bidirectional guidance molecule regulating growth cone motility.
    Article Snippet: Pharmacological agents were bath-applied to cultures 20 min prior to growth cone turning assay as listed: chlorpromazine (5 μM,Sigma-Aldrich), ritanserin (1 nM, Tocris), GR-55562 dihydrochloride (500 nM, R&D Systems), SpcAMPs (20 μM, BioLog Life Science), U-731222 (20 nM, Sigma-Aldrich), forskolin (5 μM, Sigma-Aldrich), nifedipine 1 3 (5 μM, Alomone Labs), thapsigargin (50 nM, Alomone Labs), ondansentron (10 nM, Tocris), TCB-2 ((4-Bromo3,6-dimethoxybenzocyclobuten-1-yl)methylamine hydrobromide, 2 nM, Tocris), CP 94253 hydrochloride (5-Propoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1H-pyrrolo[3,2-b] pyridine hydrochloride, 5 nM, Tocris). .. DRG sensory neurons were fixed with paraformaldehyde (PFA) (4%, Sigma-Aldrich) in phosphate buffered saline (PBS) overnight at 4 °C, washed in PBS (3 × 10 min), for some experiments, cells were permeabilised for 1 h with blocking solution (PBS containing 0.4% Triton X-100; 5% goat serum, Sigma-Aldrich & Gibco) and immunostained with a rabbit antibody to serotonin receptors 5-HT1b (1:500; Abcam) or 5-HT2a (1:500; Alomone Labs) in blocking solution overnight at 4 °C. .. Following washes in PBS (3 × 10 min), Alexa Fluor goat anti-rabbit 488/568/647 secondary antibodies (1:1000, Invitrogen) were incubated for 1 h and rinsed in PBS (3x10 min) prior to 4% PFA incubation overnight at 4 °C.

    Blocking Assay:

    Article Title: Serotonin functions as a bidirectional guidance molecule regulating growth cone motility
    Article Snippet: Pharmacological agents were bath-applied to cultures 20 min prior to growth cone turning assay as listed: chlorpromazine (5 μM,Sigma-Aldrich), ritanserin (1 nM, Tocris), GR-55562 dihydrochloride (500 nM, R&D Systems), Sp-cAMPs (20 μM, BioLog Life Science), U-731222 (20 nM, Sigma-Aldrich), forskolin (5 μM, Sigma-Aldrich), nifedipine (5 μM, Alomone Labs), thapsigargin (50 nM, Alomone Labs), ondansentron (10 nM, Tocris), TCB-2 ((4-Bromo-3,6-dimethoxybenzocyclobuten-1-yl)methylamine hydrobromide, 2 nM, Tocris), CP 94253 hydrochloride (5-Propoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1H-pyrrolo[3,2-b]pyridine hydrochloride, 5 nM, Tocris). .. DRG sensory neurons were fixed with paraformaldehyde (PFA) (4%, Sigma-Aldrich) in phosphate buffered saline (PBS) overnight at 4 °C, washed in PBS (3 × 10 min), for some experiments, cells were permeabilised for 1 h with blocking solution (PBS containing 0.4% Triton X-100; 5% goat serum, Sigma-Aldrich & Gibco) and immunostained with a rabbit antibody to serotonin receptors 5-HT1b (1:500; Abcam) or 5-HT2a (1:500; Alomone Labs) in blocking solution overnight at 4 °C. .. Following washes in PBS (3 × 10 min), Alexa Fluor goat anti-rabbit 488/568/647 secondary antibodies (1:1000, Invitrogen) were incubated for 1 h and rinsed in PBS (3x10 min) prior to 4% PFA incubation overnight at 4 °C.

    Article Title: Serotonin functions as a bidirectional guidance molecule regulating growth cone motility
    Article Snippet: Pharmacological agents Pharmacological agents were bath-applied to cultures 20 min prior to growth cone turning assay as listed: chlorpromazine (5 μM,Sigma-Aldrich), ritanserin (1 nM, Tocris), GR-55562 dihydrochloride (500 nM, R&D Systems), Sp-cAMPs (20 μM, BioLog Life Science), U-731222 (20 nM, Sigma-Aldrich), forskolin (5 μM, Sigma-Aldrich), nifedipine (5 μM, Alomone Labs), thapsigargin (50 nM, Alomone Labs), ondansentron (10 nM, Tocris), TCB-2 ((4-Bromo-3,6-dimethoxybenzocyclobuten-1-yl)methylamine hydrobromide, 2 nM, Tocris), CP 94253 hydrochloride (5-Propoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1H-pyrrolo[3,2-b]pyridine hydrochloride, 5 nM, Tocris). .. Immunocytochemistry DRG sensory neurons were fixed with paraformaldehyde (PFA) (4%, Sigma-Aldrich) in phosphate buffered saline (PBS) overnight at 4 °C, washed in PBS (3 × 10 min), for some experiments, cells were permeabilised for 1 h with blocking solution (PBS containing 0.4% Triton X-100; 5% goat serum, Sigma-Aldrich & Gibco) and immunostained with a rabbit antibody to serotonin receptors 5-HT1b (1:500; Abcam) or 5-HT2a (1:500; Alomone Labs) in blocking solution overnight at 4 °C. .. Following washes in PBS (3 × 10 min), Alexa Fluor goat anti-rabbit 488/568/647 secondary antibodies (1:1000, Invitrogen) were incubated for 1 h and rinsed in PBS (3x10 min) prior to 4% PFA incubation overnight at 4 °C.

    Article Title: Serotonin functions as a bidirectional guidance molecule regulating growth cone motility.
    Article Snippet: Pharmacological agents were bath-applied to cultures 20 min prior to growth cone turning assay as listed: chlorpromazine (5 μM,Sigma-Aldrich), ritanserin (1 nM, Tocris), GR-55562 dihydrochloride (500 nM, R&D Systems), SpcAMPs (20 μM, BioLog Life Science), U-731222 (20 nM, Sigma-Aldrich), forskolin (5 μM, Sigma-Aldrich), nifedipine 1 3 (5 μM, Alomone Labs), thapsigargin (50 nM, Alomone Labs), ondansentron (10 nM, Tocris), TCB-2 ((4-Bromo3,6-dimethoxybenzocyclobuten-1-yl)methylamine hydrobromide, 2 nM, Tocris), CP 94253 hydrochloride (5-Propoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1H-pyrrolo[3,2-b] pyridine hydrochloride, 5 nM, Tocris). .. DRG sensory neurons were fixed with paraformaldehyde (PFA) (4%, Sigma-Aldrich) in phosphate buffered saline (PBS) overnight at 4 °C, washed in PBS (3 × 10 min), for some experiments, cells were permeabilised for 1 h with blocking solution (PBS containing 0.4% Triton X-100; 5% goat serum, Sigma-Aldrich & Gibco) and immunostained with a rabbit antibody to serotonin receptors 5-HT1b (1:500; Abcam) or 5-HT2a (1:500; Alomone Labs) in blocking solution overnight at 4 °C. .. Following washes in PBS (3 × 10 min), Alexa Fluor goat anti-rabbit 488/568/647 secondary antibodies (1:1000, Invitrogen) were incubated for 1 h and rinsed in PBS (3x10 min) prior to 4% PFA incubation overnight at 4 °C.

    Article Title: Abdominal Ultrasound Stimulation Alleviates Negative Symptoms Through Modulation of Serotonin Signaling and Gut Microbiota in the MK-801 Model of Schizophrenia.
    Article Snippet: © The Author(s) 2025.. esearch Center. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints.

    Article Title: Serotonin-Mediated Cardiac Analgesia via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction.
    Article Snippet: PII: S0306-4522(19)30375-6 DOI: https://doi.org/10.1016/j.neuroscience.2019.05.045 Reference: NSC 19091 To appear in: Neuroscience Received date: 26 February 2019 Accepted date: 22 May 2019 Please cite this article as: X. Wen, X. Yu, R. Huo, et al., Serotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction, Neuroscience, https://doi.org/10.1016/ j.neuroscience.2019.05.045 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Immunocytochemistry:

    Article Title: Serotonin functions as a bidirectional guidance molecule regulating growth cone motility
    Article Snippet: Pharmacological agents Pharmacological agents were bath-applied to cultures 20 min prior to growth cone turning assay as listed: chlorpromazine (5 μM,Sigma-Aldrich), ritanserin (1 nM, Tocris), GR-55562 dihydrochloride (500 nM, R&D Systems), Sp-cAMPs (20 μM, BioLog Life Science), U-731222 (20 nM, Sigma-Aldrich), forskolin (5 μM, Sigma-Aldrich), nifedipine (5 μM, Alomone Labs), thapsigargin (50 nM, Alomone Labs), ondansentron (10 nM, Tocris), TCB-2 ((4-Bromo-3,6-dimethoxybenzocyclobuten-1-yl)methylamine hydrobromide, 2 nM, Tocris), CP 94253 hydrochloride (5-Propoxy-3-(1,2,3,6-tetrahydro-4-pyridinyl)-1H-pyrrolo[3,2-b]pyridine hydrochloride, 5 nM, Tocris). .. Immunocytochemistry DRG sensory neurons were fixed with paraformaldehyde (PFA) (4%, Sigma-Aldrich) in phosphate buffered saline (PBS) overnight at 4 °C, washed in PBS (3 × 10 min), for some experiments, cells were permeabilised for 1 h with blocking solution (PBS containing 0.4% Triton X-100; 5% goat serum, Sigma-Aldrich & Gibco) and immunostained with a rabbit antibody to serotonin receptors 5-HT1b (1:500; Abcam) or 5-HT2a (1:500; Alomone Labs) in blocking solution overnight at 4 °C. .. Following washes in PBS (3 × 10 min), Alexa Fluor goat anti-rabbit 488/568/647 secondary antibodies (1:1000, Invitrogen) were incubated for 1 h and rinsed in PBS (3x10 min) prior to 4% PFA incubation overnight at 4 °C.

    Control:

    Article Title: Serotonin-Mediated Cardiac Analgesia via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction.
    Article Snippet: PII: S0306-4522(19)30375-6 DOI: https://doi.org/10.1016/j.neuroscience.2019.05.045 Reference: NSC 19091 To appear in: Neuroscience Received date: 26 February 2019 Accepted date: 22 May 2019 Please cite this article as: X. Wen, X. Yu, R. Huo, et al., Serotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction, Neuroscience, https://doi.org/10.1016/ j.neuroscience.2019.05.045 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Incubation:

    Article Title: Abdominal Ultrasound Stimulation Alleviates Negative Symptoms Through Modulation of Serotonin Signaling and Gut Microbiota in the MK-801 Model of Schizophrenia.
    Article Snippet: © The Author(s) 2025.. esearch Center. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints.

    Article Title: Serotonin-Mediated Cardiac Analgesia via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction.
    Article Snippet: PII: S0306-4522(19)30375-6 DOI: https://doi.org/10.1016/j.neuroscience.2019.05.045 Reference: NSC 19091 To appear in: Neuroscience Received date: 26 February 2019 Accepted date: 22 May 2019 Please cite this article as: X. Wen, X. Yu, R. Huo, et al., Serotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction, Neuroscience, https://doi.org/10.1016/ j.neuroscience.2019.05.045 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.



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