sstr1 control peptide (Alomone Labs)
Structured Review

Sstr1 Control Peptide, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asr-001/pmc06957562-39-14-20?v=Alomone+Labs
Average 92 stars, based on 1 article reviews
Images
1) Product Images from "Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex"
Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex
Journal: Brain Structure & Function
doi: 10.1007/s00429-019-02011-7
Figure Legend Snippet: Localisation of SSTRs in the cortical layers of mice primary somatosensory cortex. SSTR1 showed the most intense signal in L4 and a weaker signal in L2/3 and L5/6. SSTR2 was localised mainly in deep cortical layers (L5-6). SSTR3, SSTR4, and SSTR5 were distributed evenly throughout the cortical layers (column A and C). Column a present representative coronal brain sections through somatosensory cortex immunofluorescently labelled with antibodies specific for SSTR1-SSTR5. Column b presents the validation of antibody specificity. Coronal brain sections were treated with primary antibodies (anti-SSTR1-5) preincubated with control blocking peptides, followed by specific secondary antibodies. The addition of control peptides resulted in the disappearance of the antibody-specific signal. Scale bar 100 μm
Techniques Used: Blocking Assay
Figure Legend Snippet: SSTRs localisation in the somatosensory cortex of mice. High magnification confocal pictures of coronal sections presenting localisation of SSTR1-5 in the mouse somatosensory cortex. a SSTR1 labelled clearly the pyramidal-like cell bodies and apical dendrites. b Lack of co-localisation of SSTR2s and PV INs in the mouse primary somatosensory cortex. Yellow lines represent two orthogonal sections of a z-series showing the distribution of green and red fluorescence in the tissue. c SSTR3 immunoreactivity was found in cell bodies and neuropil. d, e Immunofluorescent signal for SSTR4 and SSTR5 was found mainly in the neuropil and much less visible in cell bodies. Scale bar for all pictures: 10 μm
Techniques Used: Fluorescence
Figure Legend Snippet: Two types of SSTRs distribution in the mouse barrel field. Tangential brain sections through L4 of the mouse barrel cortex. Type A distribution is characterised by a high concentration of immunoreactivity in the barrel walls (sides + septa). This type is represented by SSTR1 and SSTR2. Type B distribution is characterised by a homogeneous intensity of immunoreactivity in the barrel hollows. This type is represented by SSTR3, SSTR4, and SSTR5. Scale bar: 100 μm. Nuclear DAPI staining is visible in blue
Techniques Used: Concentration Assay, Staining
Figure Legend Snippet: Quantitative assessment of co-localisation of SSTRs with PV, SST, and VIP containing INs in the particular cortical layers of the mouse somatosensory cortex. Graphs represent the percentage (mean + SD) of all labelled interneurons of each particular type that exhibited co-localisation with SSTRs in different cortical layers. Top panel: SSTR1-5 + PV INs, Middle panel: SSTR1-5 + SST INs, Bottom panel: SSTR1-5 + VIP INs. Kruskal–Wallis test, * p ≤ 0.05, # 0.05 < p < 0.051; n = 3
Techniques Used: