rabbit anti at1r  (Alomone Labs)

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Localisation of SSTRs in the cortical layers of mice primary somatosensory cortex. SSTR1 showed the most intense signal in L4 and a weaker signal in L2/3 and L5/6. SSTR2 was localised mainly in deep cortical layers (L5-6). SSTR3, SSTR4, and SSTR5 were distributed evenly throughout the cortical layers (column A and C). Column a present representative coronal brain sections through somatosensory cortex immunofluorescently labelled with antibodies specific for SSTR1-SSTR5. Column b presents the validation of antibody specificity. Coronal brain sections were treated with primary antibodies (anti-SSTR1-5) preincubated with control blocking peptides, followed by specific secondary antibodies. The addition of control peptides resulted in the disappearance of the antibody-specific signal. Scale bar 100 μm

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques: Biomarker Discovery, Control, Blocking Assay

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: SSTRs localisation in the somatosensory cortex of mice. High magnification confocal pictures of coronal sections presenting localisation of SSTR1-5 in the mouse somatosensory cortex. a SSTR1 labelled clearly the pyramidal-like cell bodies and apical dendrites. b Lack of co-localisation of SSTR2s and PV INs in the mouse primary somatosensory cortex. Yellow lines represent two orthogonal sections of a z-series showing the distribution of green and red fluorescence in the tissue. c SSTR3 immunoreactivity was found in cell bodies and neuropil. d, e Immunofluorescent signal for SSTR4 and SSTR5 was found mainly in the neuropil and much less visible in cell bodies. Scale bar for all pictures: 10 μm

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques: Fluorescence

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Two types of SSTRs distribution in the mouse barrel field. Tangential brain sections through L4 of the mouse barrel cortex. Type A distribution is characterised by a high concentration of immunoreactivity in the barrel walls (sides + septa). This type is represented by SSTR1 and SSTR2. Type B distribution is characterised by a homogeneous intensity of immunoreactivity in the barrel hollows. This type is represented by SSTR3, SSTR4, and SSTR5. Scale bar: 100 μm. Nuclear DAPI staining is visible in blue

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques: Concentration Assay, Staining

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Quantitative assessment of co-localisation of SSTRs with PV, SST, and VIP containing INs in the particular cortical layers of the mouse somatosensory cortex. Graphs represent the percentage (mean + SD) of all labelled interneurons of each particular type that exhibited co-localisation with SSTRs in different cortical layers. Top panel: SSTR1-5 + PV INs, Middle panel: SSTR1-5 + SST INs, Bottom panel: SSTR1-5 + VIP INs. Kruskal–Wallis test, * p ≤ 0.05, # 0.05 < p < 0.051; n = 3

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques:

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Localisation of SSTRs in the cortical layers of mice primary somatosensory cortex. SSTR1 showed the most intense signal in L4 and a weaker signal in L2/3 and L5/6. SSTR2 was localised mainly in deep cortical layers (L5-6). SSTR3, SSTR4, and SSTR5 were distributed evenly throughout the cortical layers (column A and C). Column a present representative coronal brain sections through somatosensory cortex immunofluorescently labelled with antibodies specific for SSTR1-SSTR5. Column b presents the validation of antibody specificity. Coronal brain sections were treated with primary antibodies (anti-SSTR1-5) preincubated with control blocking peptides, followed by specific secondary antibodies. The addition of control peptides resulted in the disappearance of the antibody-specific signal. Scale bar 100 μm

Article Snippet: The blocking peptides used in this research to confirm antibody specificity were as follows: SSTR1 control peptide (catalogue no. ASR-001, Alomone Labs), SSTR2 control peptide (catalogue no. ab171899, Abcam), SSTR3 control peptide (catalogue no. ASR-003, Alomone Labs), peptide corresponding to the anti-SSTR4 antibody (amino acid sequence (C) QQEALQPEPGRKRIPLTRTTTF, Ontores, commercially not available), and SSTR5 control peptide (catalogue no. ASR-005, Alomone Labs).

Techniques: Blocking Assay

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: SSTRs localisation in the somatosensory cortex of mice. High magnification confocal pictures of coronal sections presenting localisation of SSTR1-5 in the mouse somatosensory cortex. a SSTR1 labelled clearly the pyramidal-like cell bodies and apical dendrites. b Lack of co-localisation of SSTR2s and PV INs in the mouse primary somatosensory cortex. Yellow lines represent two orthogonal sections of a z-series showing the distribution of green and red fluorescence in the tissue. c SSTR3 immunoreactivity was found in cell bodies and neuropil. d, e Immunofluorescent signal for SSTR4 and SSTR5 was found mainly in the neuropil and much less visible in cell bodies. Scale bar for all pictures: 10 μm

Article Snippet: The blocking peptides used in this research to confirm antibody specificity were as follows: SSTR1 control peptide (catalogue no. ASR-001, Alomone Labs), SSTR2 control peptide (catalogue no. ab171899, Abcam), SSTR3 control peptide (catalogue no. ASR-003, Alomone Labs), peptide corresponding to the anti-SSTR4 antibody (amino acid sequence (C) QQEALQPEPGRKRIPLTRTTTF, Ontores, commercially not available), and SSTR5 control peptide (catalogue no. ASR-005, Alomone Labs).

Techniques: Fluorescence

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Two types of SSTRs distribution in the mouse barrel field. Tangential brain sections through L4 of the mouse barrel cortex. Type A distribution is characterised by a high concentration of immunoreactivity in the barrel walls (sides + septa). This type is represented by SSTR1 and SSTR2. Type B distribution is characterised by a homogeneous intensity of immunoreactivity in the barrel hollows. This type is represented by SSTR3, SSTR4, and SSTR5. Scale bar: 100 μm. Nuclear DAPI staining is visible in blue

Article Snippet: The blocking peptides used in this research to confirm antibody specificity were as follows: SSTR1 control peptide (catalogue no. ASR-001, Alomone Labs), SSTR2 control peptide (catalogue no. ab171899, Abcam), SSTR3 control peptide (catalogue no. ASR-003, Alomone Labs), peptide corresponding to the anti-SSTR4 antibody (amino acid sequence (C) QQEALQPEPGRKRIPLTRTTTF, Ontores, commercially not available), and SSTR5 control peptide (catalogue no. ASR-005, Alomone Labs).

Techniques: Concentration Assay, Staining

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Quantitative assessment of co-localisation of SSTRs with PV, SST, and VIP containing INs in the particular cortical layers of the mouse somatosensory cortex. Graphs represent the percentage (mean + SD) of all labelled interneurons of each particular type that exhibited co-localisation with SSTRs in different cortical layers. Top panel: SSTR1-5 + PV INs, Middle panel: SSTR1-5 + SST INs, Bottom panel: SSTR1-5 + VIP INs. Kruskal–Wallis test, * p ≤ 0.05, # 0.05 < p < 0.051; n = 3

Article Snippet: The blocking peptides used in this research to confirm antibody specificity were as follows: SSTR1 control peptide (catalogue no. ASR-001, Alomone Labs), SSTR2 control peptide (catalogue no. ab171899, Abcam), SSTR3 control peptide (catalogue no. ASR-003, Alomone Labs), peptide corresponding to the anti-SSTR4 antibody (amino acid sequence (C) QQEALQPEPGRKRIPLTRTTTF, Ontores, commercially not available), and SSTR5 control peptide (catalogue no. ASR-005, Alomone Labs).

Techniques: