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Journal: The Journal of Comparative Neurology
Article Title: Distribution of Voltage‐Gated Sodium Channels and Scaffolding Proteins on Vestibular Calyx Ending Delineates the Axon Initial Segment
doi: 10.1002/cne.70127
Figure Lengend Snippet: Na V 1.3 antibody labels Domain 3 in presumed C units. (A, A’) In this maximum intensity projection image of a stack from a crista, the presumption is based on the relatively superficial location of Caspr1‐stained hemi‐paranodes (red, arrows) and the location of the units in the CZ. Na V 1.3 labeling ( green in A’) is absent in the PZ ( not shown ), which contains D , but not C , units. In the three units marked by arrows in A, MBP ( blue ) can be traced from the parent axons below the basement membrane (BM) (dashed line) to the stained hemi‐paranodes. In A’, Caspr1 immunostaining ( red ) has been removed, leaving Na V 1.3 labeling ( green ) of Domain 3, including the outer surface of the calyx endings (COF, Domain 3a) and the unmyelinated parent axons above the heminodes (Domain 3b, locations indicated by arrows ). Inset, upper right , schematic of entire calyx, showing Domain 3 in red. (B, C) Additional examples of calyx afferents labeled with Na V 1.3. Same color conventions as in A. Scale bars = 5 µm.
Article Snippet:
Techniques: Staining, Labeling, Membrane, Immunostaining