apc-conjugated pro5 mhc np 147-155 pentamer (Proimmune)
90
Structured Review
Proimmune
apc-conjugated pro5 mhc np 147-155 pentamer
Apc Conjugated Pro5 Mhc Np 147 155 Pentamer, supplied by Proimmune, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/apc-155/apc+pro5+mhc+pentamer+h2db+asmtnmelm/pmc12003732-392-5-7
Average 90 stars, based on 1 article reviews
Apc Conjugated Pro5 Mhc Np 147 155 Pentamer, supplied by Proimmune, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/apc-155/apc+pro5+mhc+pentamer+h2db+asmtnmelm/pmc12003732-392-5-7
Average 90 stars, based on 1 article reviews
apc-conjugated pro5 mhc np 147-155 pentamer - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice"
Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice
Journal: Nature Communications
doi: 10.1038/s41467-025-58931-y
Figure Legend Snippet: A On day 0, seven-weeks-old female BALB/c mice were either i.n. immunized with rAd-HA, rAd-NP, and rAd-IL-1β (each 2 × 10 8 particles) or infected with the H1N1 strain A/PR/8/34 (100 PFU). At the indicated time points, lymphocytes were isolated from lung tissues to evaluate T-cell phenotypes and functional responses. In addition, blood and BALF samples were collected at the specified times to assess systemic and local antibody responses. The localization and organization of different immune cells was examined by histological techniques. B At the indicated times, antigen-specific cells were identified by pentamer staining, and total numbers of HA 533-541 - and NP 147-155 -specific Pent + cells are depicted. The distribution of antigen-specific T cells within the iv - and iv + proportions are illustrated by different coloring. C Absolute numbers of circulating effector and memory T-cell subsets, and those of the T RM pool induced by mucosal vaccination or H1N1 infection are shown. Iv - T RM (KLRG1-) are colored in different shades of blue (regarding CD69 and CD103 expression), while effector T cells (T eff ; iv + KLRG1 + CD127 - ) are shown in black, and effector memory T cells (T EM ; iv + KLRG1 + CD127 + ), and central memory T cells (T CM ; iv + KLRG1- CD127 + ) are shown in gray. Each data set represents the mean + SEM of per group ( n = 6 mice per group/timepoint, except 150 dpi in the H1N1 group represents only n = 4). Statistical significances were analyzed by two-way ANOVA followed by Šídák’s multiple comparison test (*, p < 0.1; **, p < 0.01; ***, p < 0.001 (gray line: iv - )). ( A ) Created in BioRender. Tenbusch, M. (2025) https://BioRender.com/o92n191 .
Techniques Used: Infection, Isolation, Functional Assay, Staining, Expressing, Comparison
Figure Legend Snippet: A Lungs from rAd-immunized or H1N1-infected mice were obtained 18 days post-treatment, and HE-stained lung sections were scanned at 40x magnification. Airways were generally intact and solely H1N1 infection caused lymphocytic infiltration reflected by areas of lymphocyte accumulations, without neutrophils, monocytes, and macrophages (arrows). One representative example of four mice per group is shown. Scale bars in whole slide lung images are 1000 µm and scale bars in magnified images are 100 µm. B At the indicated time points, lungs of naïve, immunized, and infected mice were removed, embedded into O.C.T. compound, and finally stained with anti-CD4, anti-CD8, and anti-B220. Nuclei are shown in blue (Hoechst33342), CD4 + T cells are shown in yellow, CD8 + T cells are shown in green, and B cells are shown in red. C To identify the localization of antigen-specific CD8 + T cells in the lung parenchyma, lung sections from day 18 p.i. were primary stained with anti-CD8, followed by MHC-I pentamer staining. The signal intensity of bound APC-labeled pentamers was amplified with anti-APC-AF647, and anti-mouse IgG2b-AF647. Nuclei are shown in blue (Hoechst33342), CD8 + T cells are shown in green, and MHC-I pentamer-specific T cells are shown in red. Images were acquired on a Leica SP5X laser scanning confocal microscope using a 40x oil objective and are representatives from a collection of different slices of four mice per group ( n = 4). Scale bars in each image are 50 µm.
Techniques Used: Infection, Staining, Labeling, Amplification, Microscopy
Figure Legend Snippet: Previously, rAd-immunized or H1N1-infected mice were challenged with H3N2 (10,000 PFU) 56 days after the initial prime, and lymphocytes from lung tissues were isolated 44 days after the challenge (+ H3N2). One set of mice was not reinfected and served as the control group (- H3N2). Additionally, some lymphocytes were restimulated in vitro using MHC-II- or MHC-I-restricted peptides derived from HA and NP, and ICS was used for the functional identification. A , B Phenotypic differentiation between antigen-specific T eff , T EM , and T CM , and different subsets of T RM . The graphs show the total number of HA 533-541 - and NP 147-155 -specific Pent + CD8 + T cells. C Frequencies of cytokine-specific CD8 + T cells investigated in secondary infected mice (+ H3N2) compared to only primed mice (- H3N2) are shown. A – C Each data point represents an individual mouse, and bars represent the mean of the group ( n = 6 mice for rAd and H1N1 (-H3N2), 7 mice for H1N1 (+ H3N2) and 8 mice for rAd (+ H3N2). To compare statistical effects between unchallenged (- H3N2) and challenged (+ H3N2) mice of one group, statistical significances were analyzed by two-tailed Mann-Whitney test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).
Techniques Used: Infection, Isolation, Control, In Vitro, Derivative Assay, Functional Assay, Two Tailed Test, MANN-WHITNEY
Figure Legend Snippet: A The survival of previously induced IAV-specific T RM was examined upon secondary inflammatory events by subsequent infection with the unrelated RSV (1 × 10 6 PFU) or by LPS treatment (10 µg) on day 56 after priming. Three weeks after the challenge, lymphocytes were isolated from lung tissues. One set of initially primed mice remained without secondary exposure (- RSV, - LPS, respectively) and served as the control group. B Body weight was measured for nine days following the RSV challenge, and values represent percentages of the initial weight on day 0. C Antigen-specific CD8 + T cells were identified by pentamer staining. Total numbers of HA 533-541 -, NP 147-155 -, and M2 82-90 -specific tissue-resident CD8 + T cells are shown. D Frequencies of non-circulating CD8 + T cells that are at least positive for the single expression of either CD107a, IFNγ, IL-2, or TNFα were assessed after in vitro restimulation. E Immunofluorescence staining of lung tissues from initially rAd-immunized or H1N1-infected mice reinfected with RSV on day 35 and collected an additional 35 days after challenge. Tissue sections were stained with anti-B220 (green), RSV M2 82-90 pentamer (yellow), and IAV NP 147-155 pentamer (red). Nuclei are shown in blue (Hoechst33342). Images were acquired on a Leica SP5X laser scanning confocal microscope using a 40x oil objective and are representatives from a collection of different slices of three mice per group ( n = 3). Scale bars in each image are 50 µm. C , D Each data point represents an individual mouse, and bars represent the mean of the group mean ± SEM per group ( n = 6 mice per group, only in (D) one sample in the group rAd (-RSV) was lost due to technical issues). Numbers above the columns show fold change between unchallenged (- RSV) and challenged (+ RSV) mice. Statistical significances were analyzed ( B) by two-way ANOVA followed by Tukey’s multiple comparison test, or ( C , D ) by one-way ANOVA followed by Tukey’s multiple comparison test (( B ): *, p < 0.05 H1N1 + RSV vs. naïve + RSV; ( C , D ); *, p < 0.1; ***, p < 0.001; ****, p < 0.001). ( A ) Created in BioRender. Tenbusch, M. (2025) https://BioRender.com/g83h450 .
Techniques Used: Infection, Isolation, Control, Staining, Expressing, In Vitro, Immunofluorescence, Microscopy, Comparison
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Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Amplification:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Microscopy:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Isolation:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Control:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP In Vitro:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Derivative Assay:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Functional Assay:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Two Tailed Test:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP MANN-WHITNEY:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Expressing:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Immunofluorescence:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Comparison:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Multiplex Assay:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Sequencing:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Enzyme-linked Immunosorbent Assay:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Enzyme-linked Immunospot:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP Flow Cytometry:Article Title: Colocalized inhibition of TGF-β and PD-L1 Article Snippet: For P15E staining, MC38 tumor cells were incubated with Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice. Article Snippet: Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells. Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy. Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma528,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells Article Snippet: For the flow cytometric detection of OVA-specific T cells, R-PE labeled Pro5 MHC pentamer antibody specific for H-2Kb SIINFLEKL was used according to manufacturer’s instructions ( Article Title: NitraTh epitope-based neoantigen vaccines for effective tumor immunotherapy Article Snippet: The antibodies were utilized in this study: FITC anti-mouse CD45 (Biolegend, Cat. No. 103108), PE anti-mouse CD3 (Biolegend, Cat. No. 100206), BB700 anti-mouse CD3e (BD, Cat. No. 566494), FITC anti-mouse CD4 (Biolegend, Cat. No. 100406), BV605 anti-mouse CD4 (BD, Cat. No. 563151), PE-CY7 anti-mouse CD8 (BD, Cat. No. 552877), APC anti-mouse CD107a (eBioscience, Cat. No. ma5-28,671), APC anti-mouse CD25 (BD, Cat. No. 557192), APC anti-mouse IFN-γ (BD, Cat. No. 554413), BV786 anti-mouse CD366 (BD, Cat. No. 747621), PE FOXP3 Monoclonal Antibody (eBioscience, Cat. No. 12–5773-82), PE-CY7 anti-mouse CD11B (BD, Cat. No. 552850), BV480 anti-mouse F4/80 (BD, Cat. No. 565635), Super Bright 436 anti-mouse Ly6c (eBioscience, Cat. No. 62–5932-82), Article Title: Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice Article Snippet: Similarly, APC-conjugated Pro5 MHC NP |