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anti kcnn3 kca2 3  (Alomone Labs)


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    Alomone Labs anti kcnn3 kca2 3
    Anti Kcnn3 Kca2 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc-025/Anti-KCNN3+Antibody/bio_rxiv__2025__07__29__667377-304-11-13
    Average 95 stars, based on 79 article reviews
    anti kcnn3 kca2 3 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: High-resolution integrative analysis allows characterization and spatial annotation of normal and cancer-associated colon fibroblasts
    Article Snippet: Blocking was performed using TNB blocking buffer (SKU FP1012, Akoya Biosciences) for 30 min at RT. .. Primary antibodies anti-KCNN3 (APC-025, Alomone Labs, 1:100 dilution) and anti-PDGFRa (AF-307-NA, R&D Systems, 1:50 dilution) were incubated overnight at +4°C. .. Secondary antibodies Alexa Fluor 647 donkey anti-rabbit (A-31573, Thermo Fisher Scientific) and Alexa Fluor 594 donkey anti-goat (A11058, Thermo Fisher Scientific) were incubated in blocking buffer (1:500 dilution) for one hour at RT and washed 3×5 min in 0.1% TBS-Tween.

    Article Title: Properties of rhythmogenic currents in spinal Shox2 interneurons across postnatal development
    Article Snippet: .. Slides were incubated in one of the following primary antibodies overnight: rabbit anti-KCNQ2 (dilution 1:1000; PA1-929; Thermo Fisher Scientific), rabbit anti-KCNQ3 (dilution 1:400; APC-051; Alomone, Jerusalem, Israel), rabbit anti-SK2 (dilution 1:50; APC-028; Alomone) or rabbit anti-SK3 (dilution 1:50; APC-025; Alomone). .. Slides were then incubated for 2 h in goat anti-rabbit 647 secondary antibody (dilution 1:400; A-21245; Invitrogen, Waltham, MA, USA).

    Article Title: High-resolution integrative analysis allows characterization and spatial annotation of normal and cancer-associated colon fibroblasts
    Article Snippet: .. The samples were incubated with anti-PDGFRa (AF1062, R&D systems, 1:100) and anti-KCNN3/KCa2.3 (APC-025, Alomone Labs, 1:400) antibodies overnight at 4°C in blocking buffer. ..

    Oligomer Restriction:

    Article Title: IK Channel Confers Fine-tuning of Rod Bipolar Cell Excitation and Synaptic Transmission in the Retina
    Article Snippet: .. Anti-KCa1.1 (1:100; APC-151, Alomone, Jerusalem, Israel), Anti-KCa2.1 (1:100; APC-039 Alomone), Anti-KCa2.2 (1:100; APC-028, Alomone), Anti-KCa2.3 (1:100; APC-025, Alomone), Anti-KCa3.1 (1:100; ALM-051, Alomone), Anti-PKCα (1:1000; sc-208, Santa Cruz, Dallas, Texas, U.S.A.), Cy3 (1:1500; Jackson Immunoresearch, West Grove, PA, USA), and Alexa 488-conjugated antibodies (1:500; Molecular Probes, Eugene, OR, USA) were used for primary and secondary antibodies. .. All images were captured using a Zeiss LSM 900 confocal microscope (Carl Zeiss Co. Ltd, Oberkochen, Germany).

    SDS Page:

    Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION
    Article Snippet: Protein concentrations were determined using a detergent-compatible assay (Bio-Rad). .. Equal amounts of protein (40 μg per lane) were separated on 4-15% gradient SDS-PAGE stain-free gels (Bio-Rad), transferred to nitrocellulose membranes, and probed overnight at 4 °C with either a polyclonal rabbit anti-SK3 antibody (1:500, Alomone Labs, APC-025) or an anti-actin antibody (1:1,000, A2066, Sigma-Aldrich) in Tris-buffered saline containing 5% fat-free milk. .. Membranes were then incubated for 1 h at room temperature with a goat anti-rabbit horseradish peroxidase-conjugated secondary antibody (1:40,000; Thermo Fisher).

    Staining:

    Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION
    Article Snippet: Protein concentrations were determined using a detergent-compatible assay (Bio-Rad). .. Equal amounts of protein (40 μg per lane) were separated on 4-15% gradient SDS-PAGE stain-free gels (Bio-Rad), transferred to nitrocellulose membranes, and probed overnight at 4 °C with either a polyclonal rabbit anti-SK3 antibody (1:500, Alomone Labs, APC-025) or an anti-actin antibody (1:1,000, A2066, Sigma-Aldrich) in Tris-buffered saline containing 5% fat-free milk. .. Membranes were then incubated for 1 h at room temperature with a goat anti-rabbit horseradish peroxidase-conjugated secondary antibody (1:40,000; Thermo Fisher).

    Saline:

    Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION
    Article Snippet: Protein concentrations were determined using a detergent-compatible assay (Bio-Rad). .. Equal amounts of protein (40 μg per lane) were separated on 4-15% gradient SDS-PAGE stain-free gels (Bio-Rad), transferred to nitrocellulose membranes, and probed overnight at 4 °C with either a polyclonal rabbit anti-SK3 antibody (1:500, Alomone Labs, APC-025) or an anti-actin antibody (1:1,000, A2066, Sigma-Aldrich) in Tris-buffered saline containing 5% fat-free milk. .. Membranes were then incubated for 1 h at room temperature with a goat anti-rabbit horseradish peroxidase-conjugated secondary antibody (1:40,000; Thermo Fisher).

    other:

    Article Title: Physiological oxygen levels reset K + channel activity in human vascular endothelial cells.
    Article Snippet: Whole cell lysates were collected from HUVEC or hCMEC/D3 cells maintained within the workstations for 5 days under 18 kPa or 5 kPa O2, using SDS lysis buffer supplemented with protease inhibitor cocktail (Sigma-Aldrich, UK).

    Blocking Assay:

    Article Title: High-resolution integrative analysis allows characterization and spatial annotation of normal and cancer-associated colon fibroblasts
    Article Snippet: .. The samples were incubated with anti-PDGFRa (AF1062, R&D systems, 1:100) and anti-KCNN3/KCa2.3 (APC-025, Alomone Labs, 1:400) antibodies overnight at 4°C in blocking buffer. ..



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    a-c Voltage traces from ventromedial interneurons of the rhythmogenic CPG region (L1-L2) recorded under control conditions ( a ), after tamapin application (10 nM, b ), or after lei-dab7 application (10 nM, c ). d Bar graph showing the proportion of interneurons displaying bursting in response to increasing concentrations of lei-dab7 (teal) or tamapin (purple). e Representative action potentials evoked by near-threshold current injections under control conditions (black), tamapin (purple), or lei-dab7 (teal). f-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying AHP amplitude ( f ), AHP duration ( g ), and firing frequency ( h ) under the three conditions. i-k Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying burst duration ( i ), amplitude ( j ), and frequency ( k ) across interneurons treated with apamin (pink), tamapin (purple), or lei-dab7 (teal). l-o Representative voltage traces from interneurons expressing control shRNA ( l ) or <t>SK3-targeting</t> shRNA ( m-o ), showing heterogeneous bursting phenotypes: bursts at rest/rheobase ( m ), bursts requiring stronger depolarization ( n ), and elliptic bursting dynamics ( o ). p-r Raincloud plots comparing burst duration ( p ), amplitude ( q ), and frequency ( r ) in interneurons after SK3 knockdown (orange) and after apamin application (pink). Numbers in parentheses denote recorded cells; each dot represents a single cell. Data points plotted beyond the dashed vertical line indicate values outside the axis range. n.s., not significant; * P < 0.05; ** P < 0.01; *** P < 0.001 (two-sided Fisher’s exact test for d ; Kruskal-Wallis with Dunn’s post hoc test versus control for f-h and i-k ; two-sided Mann-Whitney test for p-r ). For detailed P values, see Source Data.
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    a-c Voltage traces from ventromedial interneurons of the rhythmogenic CPG region (L1-L2) recorded under control conditions ( a ), after tamapin application (10 nM, b ), or after lei-dab7 application (10 nM, c ). d Bar graph showing the proportion of interneurons displaying bursting in response to increasing concentrations of lei-dab7 (teal) or tamapin (purple). e Representative action potentials evoked by near-threshold current injections under control conditions (black), tamapin (purple), or lei-dab7 (teal). f-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying AHP amplitude ( f ), AHP duration ( g ), and firing frequency ( h ) under the three conditions. i-k Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying burst duration ( i ), amplitude ( j ), and frequency ( k ) across interneurons treated with apamin (pink), tamapin (purple), or lei-dab7 (teal). l-o Representative voltage traces from interneurons expressing control shRNA ( l ) or <t>SK3-targeting</t> shRNA ( m-o ), showing heterogeneous bursting phenotypes: bursts at rest/rheobase ( m ), bursts requiring stronger depolarization ( n ), and elliptic bursting dynamics ( o ). p-r Raincloud plots comparing burst duration ( p ), amplitude ( q ), and frequency ( r ) in interneurons after SK3 knockdown (orange) and after apamin application (pink). Numbers in parentheses denote recorded cells; each dot represents a single cell. Data points plotted beyond the dashed vertical line indicate values outside the axis range. n.s., not significant; * P < 0.05; ** P < 0.01; *** P < 0.001 (two-sided Fisher’s exact test for d ; Kruskal-Wallis with Dunn’s post hoc test versus control for f-h and i-k ; two-sided Mann-Whitney test for p-r ). For detailed P values, see Source Data.
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    (A) Dot plot depicting marker genes for mouse MIFs (muscle-embedded interstitial fibroblasts). (B) GSEA of the mouse MIF signature against indicated Gene Ontology Molecular Function (GOMF) gene sets. (C) Immunofluorescent staining of PDGFRA and <t>KCNN3</t> in the muscularis propria in mouse colon and (D) small intestine. Dashed line indicates crypts. (E) Dot plot depicting marker genes for indicated human cell types. (F) GSEA of human MIF signature against indicated GOMF gene sets. (G) Normalized enrichment scores (NES) of GSEA of human MIF signature against gene sets from human colon-derived cells: PDGFRA+ cells of the smooth muscle (PaC), intestinal cells of Cajal (ICC), PDGFRA+ cells of the colon mucosa (FIB), smooth muscle cells of the colon muscle (SMC), enteric neurons from colon myenteric ganglia (EN). (H) Immunofluorescent staining of PDGFRA and KCNN3 in the muscularis propria in human colon. (I) Graphical representation of MIFs in the mouse and human colon.
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    95
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    (A) Dot plot depicting marker genes for mouse MIFs (muscle-embedded interstitial fibroblasts). (B) GSEA of the mouse MIF signature against indicated Gene Ontology Molecular Function (GOMF) gene sets. (C) Immunofluorescent staining of PDGFRA and <t>KCNN3</t> in the muscularis propria in mouse colon and (D) small intestine. Dashed line indicates crypts. (E) Dot plot depicting marker genes for indicated human cell types. (F) GSEA of human MIF signature against indicated GOMF gene sets. (G) Normalized enrichment scores (NES) of GSEA of human MIF signature against gene sets from human colon-derived cells: PDGFRA+ cells of the smooth muscle (PaC), intestinal cells of Cajal (ICC), PDGFRA+ cells of the colon mucosa (FIB), smooth muscle cells of the colon muscle (SMC), enteric neurons from colon myenteric ganglia (EN). (H) Immunofluorescent staining of PDGFRA and KCNN3 in the muscularis propria in human colon. (I) Graphical representation of MIFs in the mouse and human colon.
    Anti Kcnn3 Kca2 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc-025/Anti-KCNN3+Antibody/bio_rxiv__2025__07__29__667377-304-11-13
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    Image Search Results


    a-c Voltage traces from ventromedial interneurons of the rhythmogenic CPG region (L1-L2) recorded under control conditions ( a ), after tamapin application (10 nM, b ), or after lei-dab7 application (10 nM, c ). d Bar graph showing the proportion of interneurons displaying bursting in response to increasing concentrations of lei-dab7 (teal) or tamapin (purple). e Representative action potentials evoked by near-threshold current injections under control conditions (black), tamapin (purple), or lei-dab7 (teal). f-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying AHP amplitude ( f ), AHP duration ( g ), and firing frequency ( h ) under the three conditions. i-k Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying burst duration ( i ), amplitude ( j ), and frequency ( k ) across interneurons treated with apamin (pink), tamapin (purple), or lei-dab7 (teal). l-o Representative voltage traces from interneurons expressing control shRNA ( l ) or SK3-targeting shRNA ( m-o ), showing heterogeneous bursting phenotypes: bursts at rest/rheobase ( m ), bursts requiring stronger depolarization ( n ), and elliptic bursting dynamics ( o ). p-r Raincloud plots comparing burst duration ( p ), amplitude ( q ), and frequency ( r ) in interneurons after SK3 knockdown (orange) and after apamin application (pink). Numbers in parentheses denote recorded cells; each dot represents a single cell. Data points plotted beyond the dashed vertical line indicate values outside the axis range. n.s., not significant; * P < 0.05; ** P < 0.01; *** P < 0.001 (two-sided Fisher’s exact test for d ; Kruskal-Wallis with Dunn’s post hoc test versus control for f-h and i-k ; two-sided Mann-Whitney test for p-r ). For detailed P values, see Source Data.

    Journal: bioRxiv

    Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION

    doi: 10.64898/2026.03.19.712770

    Figure Lengend Snippet: a-c Voltage traces from ventromedial interneurons of the rhythmogenic CPG region (L1-L2) recorded under control conditions ( a ), after tamapin application (10 nM, b ), or after lei-dab7 application (10 nM, c ). d Bar graph showing the proportion of interneurons displaying bursting in response to increasing concentrations of lei-dab7 (teal) or tamapin (purple). e Representative action potentials evoked by near-threshold current injections under control conditions (black), tamapin (purple), or lei-dab7 (teal). f-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying AHP amplitude ( f ), AHP duration ( g ), and firing frequency ( h ) under the three conditions. i-k Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying burst duration ( i ), amplitude ( j ), and frequency ( k ) across interneurons treated with apamin (pink), tamapin (purple), or lei-dab7 (teal). l-o Representative voltage traces from interneurons expressing control shRNA ( l ) or SK3-targeting shRNA ( m-o ), showing heterogeneous bursting phenotypes: bursts at rest/rheobase ( m ), bursts requiring stronger depolarization ( n ), and elliptic bursting dynamics ( o ). p-r Raincloud plots comparing burst duration ( p ), amplitude ( q ), and frequency ( r ) in interneurons after SK3 knockdown (orange) and after apamin application (pink). Numbers in parentheses denote recorded cells; each dot represents a single cell. Data points plotted beyond the dashed vertical line indicate values outside the axis range. n.s., not significant; * P < 0.05; ** P < 0.01; *** P < 0.001 (two-sided Fisher’s exact test for d ; Kruskal-Wallis with Dunn’s post hoc test versus control for f-h and i-k ; two-sided Mann-Whitney test for p-r ). For detailed P values, see Source Data.

    Article Snippet: Equal amounts of protein (40 μg per lane) were separated on 4-15% gradient SDS-PAGE stain-free gels (Bio-Rad), transferred to nitrocellulose membranes, and probed overnight at 4 °C with either a polyclonal rabbit anti-SK3 antibody (1:500, Alomone Labs, APC-025) or an anti-actin antibody (1:1,000, A2066, Sigma-Aldrich) in Tris-buffered saline containing 5% fat-free milk.

    Techniques: Control, Whisker Assay, Expressing, shRNA, Knockdown, Single Cell, MANN-WHITNEY, Patch Clamp

    a Representative low-magnification confocal image of GFP-expressing Hb9 interneurons in the ventromedial spinal cord. The asterisk marks the soma of an identified interneuron. The central canal (cc) is indicated by the dashed line. Scale bar, 25 µm. b High-magnification images of the soma indicated in a , showing GFP fluorescence (top), SK2 immunolabeling (middle), and a merged GFP/SK2 overlay (bottom). Dashed lines delineate the somatic membrane. Scale bar, 10 µm. c-d Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( c ) and density ( d , clusters per µm 2 ) of SK2, SK3, and co-localized SK2/3 clusters at the somatic membrane. e Confocal images of an Hb9 interneuron soma showing GFP (left), SK3 immunolabeling (middle), and a merged GFP/SK3 overlay (right). Dashed lines delineate the somatic membrane. Scale bar, 20 µm. f Confocal images of an intracellularly recorded HB9 interneuron filled with biocytin (magenta), showing GFP immunofluorescence (green), SK2 (red), and SK3 immunolabeling (cyan), alongside a merged GFP/SK3 signal. Scale bar, 20 µm. g-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( g ) and density ( h ) of SK2, SK3, and co-localized clusters along the dendrites. Numbers in parentheses denote the number of cells ( c, d ) or dendrites ( g, h ) analyzed; each dot represents a single measurement. *** P < 0.001 (two-sided unpaired t-test for c and g ; One-way ANOVA for d and h ). For detailed P values, see Source data.

    Journal: bioRxiv

    Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION

    doi: 10.64898/2026.03.19.712770

    Figure Lengend Snippet: a Representative low-magnification confocal image of GFP-expressing Hb9 interneurons in the ventromedial spinal cord. The asterisk marks the soma of an identified interneuron. The central canal (cc) is indicated by the dashed line. Scale bar, 25 µm. b High-magnification images of the soma indicated in a , showing GFP fluorescence (top), SK2 immunolabeling (middle), and a merged GFP/SK2 overlay (bottom). Dashed lines delineate the somatic membrane. Scale bar, 10 µm. c-d Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( c ) and density ( d , clusters per µm 2 ) of SK2, SK3, and co-localized SK2/3 clusters at the somatic membrane. e Confocal images of an Hb9 interneuron soma showing GFP (left), SK3 immunolabeling (middle), and a merged GFP/SK3 overlay (right). Dashed lines delineate the somatic membrane. Scale bar, 20 µm. f Confocal images of an intracellularly recorded HB9 interneuron filled with biocytin (magenta), showing GFP immunofluorescence (green), SK2 (red), and SK3 immunolabeling (cyan), alongside a merged GFP/SK3 signal. Scale bar, 20 µm. g-h Raincloud plots with box-and-whisker overlays (median, interquartile range) quantifying the size ( g ) and density ( h ) of SK2, SK3, and co-localized clusters along the dendrites. Numbers in parentheses denote the number of cells ( c, d ) or dendrites ( g, h ) analyzed; each dot represents a single measurement. *** P < 0.001 (two-sided unpaired t-test for c and g ; One-way ANOVA for d and h ). For detailed P values, see Source data.

    Article Snippet: Equal amounts of protein (40 μg per lane) were separated on 4-15% gradient SDS-PAGE stain-free gels (Bio-Rad), transferred to nitrocellulose membranes, and probed overnight at 4 °C with either a polyclonal rabbit anti-SK3 antibody (1:500, Alomone Labs, APC-025) or an anti-actin antibody (1:1,000, A2066, Sigma-Aldrich) in Tris-buffered saline containing 5% fat-free milk.

    Techniques: Confocal, Expressing, Fluorescence, Immunolabeling, Membrane, Whisker Assay, Immunofluorescence

    (A) Dot plot depicting marker genes for mouse MIFs (muscle-embedded interstitial fibroblasts). (B) GSEA of the mouse MIF signature against indicated Gene Ontology Molecular Function (GOMF) gene sets. (C) Immunofluorescent staining of PDGFRA and KCNN3 in the muscularis propria in mouse colon and (D) small intestine. Dashed line indicates crypts. (E) Dot plot depicting marker genes for indicated human cell types. (F) GSEA of human MIF signature against indicated GOMF gene sets. (G) Normalized enrichment scores (NES) of GSEA of human MIF signature against gene sets from human colon-derived cells: PDGFRA+ cells of the smooth muscle (PaC), intestinal cells of Cajal (ICC), PDGFRA+ cells of the colon mucosa (FIB), smooth muscle cells of the colon muscle (SMC), enteric neurons from colon myenteric ganglia (EN). (H) Immunofluorescent staining of PDGFRA and KCNN3 in the muscularis propria in human colon. (I) Graphical representation of MIFs in the mouse and human colon.

    Journal: bioRxiv

    Article Title: High-resolution integrative analysis allows characterization and spatial annotation of normal and cancer-associated colon fibroblasts

    doi: 10.1101/2025.07.29.667377

    Figure Lengend Snippet: (A) Dot plot depicting marker genes for mouse MIFs (muscle-embedded interstitial fibroblasts). (B) GSEA of the mouse MIF signature against indicated Gene Ontology Molecular Function (GOMF) gene sets. (C) Immunofluorescent staining of PDGFRA and KCNN3 in the muscularis propria in mouse colon and (D) small intestine. Dashed line indicates crypts. (E) Dot plot depicting marker genes for indicated human cell types. (F) GSEA of human MIF signature against indicated GOMF gene sets. (G) Normalized enrichment scores (NES) of GSEA of human MIF signature against gene sets from human colon-derived cells: PDGFRA+ cells of the smooth muscle (PaC), intestinal cells of Cajal (ICC), PDGFRA+ cells of the colon mucosa (FIB), smooth muscle cells of the colon muscle (SMC), enteric neurons from colon myenteric ganglia (EN). (H) Immunofluorescent staining of PDGFRA and KCNN3 in the muscularis propria in human colon. (I) Graphical representation of MIFs in the mouse and human colon.

    Article Snippet: Primary antibodies anti-KCNN3 (APC-025, Alomone Labs, 1:100 dilution) and anti-PDGFRa (AF-307-NA, R&D Systems, 1:50 dilution) were incubated overnight at +4°C.

    Techniques: Marker, Staining, Derivative Assay