apc conjugated streptavidin (Jackson Immuno)

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Jackson Immuno apc conjugated streptavidin
Apc Conjugated Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 5192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/apc-016/Streptavidin/us12479914-729-22-24
Average 96 stars, based on 5192 article reviews

apc conjugated streptavidin - by Bioz Stars, 2026-10

96/100 stars

Images

Fluorescence:

Article Title: A new therapeutic approach for Parkinson’s disease: dual targeting of α-Synuclein aggregation and microglial function by the novel immunomodulator 3-Monothiopomalidomide
Article Snippet: The day after, sections were immunoreacted with the following unconjugated primary antibodies for double immunolabeling: rabbit monoclonal anti-pSer129-αSyn (1:800, Abcam, Cambridge, UK); mouse monoclonal anti-TH (1:400, Millipore, Burlington, MA, USA); goat polyclonal anti Iba-1 (1:1000; Novus Biologicals, Littleton, CO, USA); rabbit polyclonal anti TNF-α (1:500, Novus Biologicals, Littleton, CO, USA); rabbit polyclonal anti IL-10 (1:200, Abbiotec, Escondido, CA, USA); mouse monoclonal anti CD68 (1:100, Bio-Rad, Hercules, CA, USA). .. For fluorescence visualization of TH, IBA1 and CD68 a two-step indirect labelling protocol was used, while a three-step detection was performed to increase the signal of pSer129-αSyn, TNF-α and IL-10 by combining biotin-SP-conjugated IgG (1:500, Jackson Immunoresearch, West Grove, PA, USA) and streptavidin–fluorescein (1:400, Jackson Immunoresearch, West Grove, PA, USA), as previously described [ ]. .. The sections were then incubated for 5□min in DAPI diluted in PBS before being mounted in Vectashield Antifade Mounting Medium (H-1000-10, Vector Laboratories, UK).

Article Title: Corticospinal propagation of full-length TDP-43 toxicity drives brain-to-muscle pathology
Article Snippet: Sections were preincubated with a blocking solution with normal serum/BSA and then were immunoreacted with the following unconjugated primary antibodies for double immunolabeling: mouse monoclonal anti-NeuN (1:500, Abcam); rabbit polyclonal anti CIV (1:100, Thermo Fisher Scientific); rabbit monoclonal anti pTDP-43 (1:500, Thermo Fisher Scientific). .. For fluorescence visualization of NeuN and CIV a two-step indirect labelling protocol was used (Alexa Fluor 594 anti-mouse 1:500, Jackson ImmunoResearch West Grove, PA, USA; Alexa Fluor 488 anti-rabbit 1:500 Jackson ImmunoResearch, West Grove, PA, USA), whereas a three-step detection was carried out to increase the signal of pTDP-43 by combining biotin-SP-conjugated IgG (1:500, Jackson ImmunoResearch West Grove, PA, USA) and streptavidin–fluorescein (1:400, Jackson ImmunoResearch), as previously described (Boi et al., 2020; Palmas et al., 2022)(Boi et al., 2020; Palmas et al., 2022). .. Images were acquired using a spinning disk confocal microscope (Crisel Instruments) with a X63 magnification.

Labeling:

Article Title: Glycogen-dependent demixing of frog egg cytoplasm at increased crowding
Article Snippet: .. To observe the two phases and estimate partitioning of fluorescent probes, we imaged Fab fragment antibody labeled with Alexa Fluor 647 (Jackson ImmunoResearch #111–607-003), Streptavidin labeled with Alexa Fluor 647 (Jackson ImmunoResearch #016–600-084), and Phosphorylase A (Sigma-Aldrich #P1261) labeled with Pacific Blue (Thermo Fisher # P10163 ). .. Amyloglucosidase (AG) (Sigma-Aldrich #A7420) was added to extracts to a final concentration of 1.25 mg/mL (8.7 μM).

other:

Article Title: Specialized RNA decay fine-tunes monogenic antigen expression in Trypanosoma brucei.
Article Snippet: The nanoLC system was interfaced with an Orbitrap Fusion hybrid mass spectrometer (Thermo) with an EasyNano ionization source (Thermo).

Incubation:

Article Title: Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles
Article Snippet: .. The cell-gelation solution was then incubated at 37°C for 1–2 h. Gelled cells were immersed in heat denaturation buffer (200 mM sodium dodecyl sulfate, 200 mM NaCl, and 50 mM Tris, pH 6.8) for 1.5 h at 78°C and washed with excess of water for 30 min. To fluorescently label the whole proteins, immerse the gelled cells in poststaining buffer (10 mM HEPES and 150 mM NaCl, pH 7.5) twice, 30 min each time, then incubate the gelled cells with 4 μg/ml streptavidin dyes (included in cat#LD-P568-050/LD-P488-050; Cytoseen, or cat#016-540-084; Jackson ImmunoResearch) in poststaining buffer overnight. ..

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