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streptavidin apc  (Jackson Immuno)


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    Structured Review

    Jackson Immuno streptavidin apc
    Streptavidin Apc, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc-016/Allophycocyanin+(APC)+Streptavidin/pmc13043083-185-0-3
    Average 94 stars, based on 295 article reviews
    streptavidin apc - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    FACS:

    Article Title: CD200R1 immune checkpoint blockade by the first-in-human anti-CD200R1 antibody 23ME-00610: molecular mechanism and engineering of a surrogate antibody
    Article Snippet: .. Cells were then washed and the secondary anti-human IgG antibody (Jackson ImmunoResearch 016-130-084) diluted at 1:100 in FACS buffer was then added to the plate. ..

    Article Title: CD200R1 immune checkpoint blockade by the first-in-human anti-CD200R1 antibody 23ME-00610: molecular mechanism and engineering of a surrogate antibody.
    Article Snippet: .. Cells were then washed and the secondary anti-human IgG antibody (Jackson ImmunoResearch 016-130-084) diluted at 1:100 in FACS buffer was then added to the plate. ..

    Incubation:

    Article Title: ROR2 antibody compositions and related methods
    Article Snippet: .. Briefly, 0.1 ̃1×106 cells were incubated with purified (1 μg/mL) chimeric rabbit/human IgG1 on ice for 1 h. After washing twice with ice-cold flow cytometry buffer (PBS containing 1% (v/v) BSA, 0.1% sodium azide and 1 mM EDTA), the cells were incubated with a 1:500 dilution of goat anti-human IgG, Fcγ pAbs conjugated to APC (Jackson ImmunoResearch) in 100 μL flow cytometry buffer on ice for 30 min. After washing twice with ice-cold flow cytometry buffer, the cells were incubated with a 1:500 dilution of biotinylated rat anti-HA mAb 3F10 (Roche) in 100 μL flow cytometry buffer on ice for 1 h, washed as before, and stained with 2 μg/mL PE-conjugated streptavidin (BD Biosciences) on ice for 30 min. DAPI was added to a final concentration of 100 ng/mL to exclude dead cells from analysis. ..

    Purification:

    Article Title: ROR2 antibody compositions and related methods
    Article Snippet: .. Briefly, 0.1 ̃1×106 cells were incubated with purified (1 μg/mL) chimeric rabbit/human IgG1 on ice for 1 h. After washing twice with ice-cold flow cytometry buffer (PBS containing 1% (v/v) BSA, 0.1% sodium azide and 1 mM EDTA), the cells were incubated with a 1:500 dilution of goat anti-human IgG, Fcγ pAbs conjugated to APC (Jackson ImmunoResearch) in 100 μL flow cytometry buffer on ice for 30 min. After washing twice with ice-cold flow cytometry buffer, the cells were incubated with a 1:500 dilution of biotinylated rat anti-HA mAb 3F10 (Roche) in 100 μL flow cytometry buffer on ice for 1 h, washed as before, and stained with 2 μg/mL PE-conjugated streptavidin (BD Biosciences) on ice for 30 min. DAPI was added to a final concentration of 100 ng/mL to exclude dead cells from analysis. ..

    Flow Cytometry:

    Article Title: ROR2 antibody compositions and related methods
    Article Snippet: .. Briefly, 0.1 ̃1×106 cells were incubated with purified (1 μg/mL) chimeric rabbit/human IgG1 on ice for 1 h. After washing twice with ice-cold flow cytometry buffer (PBS containing 1% (v/v) BSA, 0.1% sodium azide and 1 mM EDTA), the cells were incubated with a 1:500 dilution of goat anti-human IgG, Fcγ pAbs conjugated to APC (Jackson ImmunoResearch) in 100 μL flow cytometry buffer on ice for 30 min. After washing twice with ice-cold flow cytometry buffer, the cells were incubated with a 1:500 dilution of biotinylated rat anti-HA mAb 3F10 (Roche) in 100 μL flow cytometry buffer on ice for 1 h, washed as before, and stained with 2 μg/mL PE-conjugated streptavidin (BD Biosciences) on ice for 30 min. DAPI was added to a final concentration of 100 ng/mL to exclude dead cells from analysis. ..

    Staining:

    Article Title: ROR2 antibody compositions and related methods
    Article Snippet: .. Briefly, 0.1 ̃1×106 cells were incubated with purified (1 μg/mL) chimeric rabbit/human IgG1 on ice for 1 h. After washing twice with ice-cold flow cytometry buffer (PBS containing 1% (v/v) BSA, 0.1% sodium azide and 1 mM EDTA), the cells were incubated with a 1:500 dilution of goat anti-human IgG, Fcγ pAbs conjugated to APC (Jackson ImmunoResearch) in 100 μL flow cytometry buffer on ice for 30 min. After washing twice with ice-cold flow cytometry buffer, the cells were incubated with a 1:500 dilution of biotinylated rat anti-HA mAb 3F10 (Roche) in 100 μL flow cytometry buffer on ice for 1 h, washed as before, and stained with 2 μg/mL PE-conjugated streptavidin (BD Biosciences) on ice for 30 min. DAPI was added to a final concentration of 100 ng/mL to exclude dead cells from analysis. ..

    Concentration Assay:

    Article Title: ROR2 antibody compositions and related methods
    Article Snippet: .. Briefly, 0.1 ̃1×106 cells were incubated with purified (1 μg/mL) chimeric rabbit/human IgG1 on ice for 1 h. After washing twice with ice-cold flow cytometry buffer (PBS containing 1% (v/v) BSA, 0.1% sodium azide and 1 mM EDTA), the cells were incubated with a 1:500 dilution of goat anti-human IgG, Fcγ pAbs conjugated to APC (Jackson ImmunoResearch) in 100 μL flow cytometry buffer on ice for 30 min. After washing twice with ice-cold flow cytometry buffer, the cells were incubated with a 1:500 dilution of biotinylated rat anti-HA mAb 3F10 (Roche) in 100 μL flow cytometry buffer on ice for 1 h, washed as before, and stained with 2 μg/mL PE-conjugated streptavidin (BD Biosciences) on ice for 30 min. DAPI was added to a final concentration of 100 ng/mL to exclude dead cells from analysis. ..

    Binding Assay:

    Article Title: Identification of novel anti-CD16a antibody clones for the development of effective natural killer cell engagers.
    Article Snippet: .. After repeated washing with binding buffer, the relevant detection antibodies were added, namely fluorescein isothiocyanate (FITC)-labeled goat anti-human Fc F(ab)2 fragment (Cat.: A24477, Invitrogen) for the detection of IgG1 and IgG4 antibody formats, Alexa Fluor 647-conjugated rabbit anti-His-Tag mAb (D3I1O, Cat.: 14931, Cell Signaling Technology) for the Fab, BiKE, or IgG-like BsAb, which each contain a C-terminal His-tag, or allophycocyanin (APC)-labeled streptavidin (Cat.: 016-130-084, Jackson ImmunoResearch) for detection of biotinylated antibodies. .. The FITC-labeled 3G8 antibody (Cat.: MHCD1601, ThermoFisher Scientific) was used as a reference binding control.

    Article Title: Identification of novel anti-CD16a antibody clones for the development of effective natural killer cell engagers
    Article Snippet: .. After repeated washing with binding buffer, the relevant detection antibodies were added, namely fluorescein isothiocyanate (FITC)-labeled goat anti-human Fc F(ab)2 fragment (Cat.: A24477, Invitrogen) for the detection of IgG1 and IgG4 antibody formats, Alexa Fluor 647-conjugated rabbit anti-His-Tag mAb (D3I1O, Cat.: 14931, Cell Signaling Technology) for the Fab, BiKE, or IgG-like BsAb, which each contain a C-terminal His-tag, or allophycocyanin (APC)-labeled streptavidin (Cat.: 016-130-084, Jackson ImmunoResearch) for detection of biotinylated antibodies. .. The FITC-labeled 3G8 antibody (Cat.: MHCD1601, ThermoFisher Scientific) was used as a reference binding control.



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