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anti cd63  (Novus Biologicals)


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    Novus Biologicals anti cd63
    Anti Cd63, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+mouse+monoclonal+anti+cd63+dshb+h5c6/CD63+Antibody+(H5C6)+-+BSA+Free/pmc11486831-190-182-186
    Average 95 stars, based on 57 article reviews
    anti cd63 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.
    Article Snippet: Permeabilization was performed using 0.1% Triton X-100 (Sigma-Aldrich, 9036-19-5) in PBS for 5–10 min at room temperature. .. After blocking with 3% FBS in PBS for 30 min, cells were incubated overnight with a CD63 antibody (H5C6; Novus Biologicals, NBP2-42225) diluted in the blocking buffer. .. Thereafter, cells were washed and incubated with a secondary anti-mouse Alexa Fluor 488 antibody (Invitrogen, A21110) and Phalloidin-iFluor 555 (Abcam, ab176756) for F-actin staining.

    Incubation:

    Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.
    Article Snippet: Permeabilization was performed using 0.1% Triton X-100 (Sigma-Aldrich, 9036-19-5) in PBS for 5–10 min at room temperature. .. After blocking with 3% FBS in PBS for 30 min, cells were incubated overnight with a CD63 antibody (H5C6; Novus Biologicals, NBP2-42225) diluted in the blocking buffer. .. Thereafter, cells were washed and incubated with a secondary anti-mouse Alexa Fluor 488 antibody (Invitrogen, A21110) and Phalloidin-iFluor 555 (Abcam, ab176756) for F-actin staining.

    Article Title: MicroRNA-625-3p Increases Chemosensitivity in Ovarian Cancer Cells Through Decreasing SSX2IP-Mediated Cisplatin Export in Extracellular Vesicles.
    Article Snippet: Proteins were separated on SDS–polyacrylamide gels and electrophoretically transferred to an immobilon polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). .. The membranes were incubated with Anti-SSX2IP (1:100; HPA027306; SigmaAldrich Co.), anti-CD63 (1:1000; NBP2-42225; Novus Biologicals, Centennial, CO, USA), and β-actin (loading control, 1:5000; clone AC-15, Sigma-Aldrich Co.) overnight at 4 ◦C and then incubated with the appropriate horseradish peroxidase-conjugated secondary https://doi.org/10.3390/cancers18050872 antibodies at a 1:10,000 dilution (Thermo Fisher Scientific) for 1 h at ambient temperature. .. Signals were developed using enhanced chemiluminescence detection reagents (Denville Scientific Inc., Holliston, MA, USA) and visualized on X-ray film (Fujifilm, Tokyo, Japan).

    Control:

    Article Title: MicroRNA-625-3p Increases Chemosensitivity in Ovarian Cancer Cells Through Decreasing SSX2IP-Mediated Cisplatin Export in Extracellular Vesicles.
    Article Snippet: Proteins were separated on SDS–polyacrylamide gels and electrophoretically transferred to an immobilon polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). .. The membranes were incubated with Anti-SSX2IP (1:100; HPA027306; SigmaAldrich Co.), anti-CD63 (1:1000; NBP2-42225; Novus Biologicals, Centennial, CO, USA), and β-actin (loading control, 1:5000; clone AC-15, Sigma-Aldrich Co.) overnight at 4 ◦C and then incubated with the appropriate horseradish peroxidase-conjugated secondary https://doi.org/10.3390/cancers18050872 antibodies at a 1:10,000 dilution (Thermo Fisher Scientific) for 1 h at ambient temperature. .. Signals were developed using enhanced chemiluminescence detection reagents (Denville Scientific Inc., Holliston, MA, USA) and visualized on X-ray film (Fujifilm, Tokyo, Japan).

    Western Blot:

    Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.
    Article Snippet: Following electrophoresis, proteins were transferred onto PVDF membranes, which were then blocked using Odyssey blocking buffer (LI-COR Biosciences) or 5% non-fat milk in TBS-0.1%T to minimize non-specific binding. .. The primary antibodies for Western blots used in this study were EFEMP1 (1:300, Novus Biologicals, NBP1-77040), β-Actin (1:2000, Cell Signaling Technology, 4970 or 3700S), STAT1 (1:1000, Cell Signaling Technology, 9172S), CD63 (1:1000, Novus Biologicals, NBP2-42225), CD81 (1:1000, Novus Biologicals, NB100-65805SS), vinculin (1:10,000, Sigma-Aldrich, V9131200UL) and Calnexin (1:1000, Novus Biologicals, NB100-1965SS). ..

    other:

    Article Title:
    Article Snippet: Mouse anti-human CD63 (Clone H5C6) , Novus Biologicals , Cat# NBP2-42225; RRID: AB_2884028.



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