monoclonal antibodies against cd81 (Becton Dickinson)
90
Structured Review
Becton Dickinson
monoclonal antibodies against cd81
Monoclonal Antibodies Against Cd81, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+mouse+monoclonal+anti+cd63+dshb+h5c6/anti+cd81/pmc05839777-191-0-7
Average 90 stars, based on 1 article reviews
Monoclonal Antibodies Against Cd81, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+mouse+monoclonal+anti+cd63+dshb+h5c6/anti+cd81/pmc05839777-191-0-7
Average 90 stars, based on 1 article reviews
monoclonal antibodies against cd81 - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling"
Article Title: Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling
Journal: The Journal of Cell Biology
doi: 10.1083/jcb.201703206
Figure Legend Snippet: CD63-pHluorin is sorted into acidic MVBs and released via exosomes. (a) Proposed model for the visualization of MVB–PM fusion: a pH-sensitive optical reporter (CD63-pHluorin) is quenched when facing the acidic lumen of the MVB. Upon fusion, low luminal pH is immediately neutralized, resulting in a sudden increase in fluorescent intensity. EC, extracellular. (b) Immunofluorescent colabeling of total CD63 (red) and CD63-pHluorin (green) in HeLa cells. PCC, Pearson’s correlation coefficient. (c) TIRF images of a CD63-pHluorin–expressing HeLa cell at normal and elevated intracellular pH (NH 4 Cl superfusion). On the right, a heat map revealing acidic vesicles close to the PM was obtained by subtracting the fluorescent intensity values of the normal pH from the high-pH condition. (d) EM images of an MVB close to the PM (left) and EVs aligning the PM (right) labeled with gold particles directed to GFP (10 nm) in CD63-pHluorin–expressing HeLa cells. (e) Imaging flow cytometry of the number of late endosomes per cell in a 2.5-µm optical section in CD63-pHluorin–expressing cells (left) or immunostaining against LAMP1 in nontransfected cells (right; n > 2,000 cells). (f) Volume distribution of endosomes based on analysis of whole-cell confocal scans (error bars represent SD; n = 3). The blue area accounts for 75% of the total number of endosomes and covers the 400–600-nm-diameter range. (g) Immunogold labeling on purified exosomes with gold particles (10 nm) coupled to anti-GFP antibody. (h) Western blotting analysis on untransfected (−) and CD63-pHluorin–transfected (+) cells and purified exosomes for total CD63 and GFP. (i) Example of a localized sudden increase in fluorescence at the PM before the event (1), during the event (2), and right before disappearance of the signal (3). (j) Left: total projection of fusion events (bright spots) over a time course of 3 min onto two cells (blue). Right: representative example of CD63-pHluorin–expressing HeLa cell. N, nucleus. Bars: (b, c, and j) 10 µm; (i) 2.5 µm. (k) Effect of incubation with GW4896 (5 µM; n ≥ 8 cells per condition) and nSMase-2 knockdown ( n ≥ 22 cells per condition) on fusion activity in HeLa cells. *, P < 0.05; ***, P < 0.001 using Student’s two-tailed two-sample t test. Whiskers in the box plots represent 1.5 times the interquartile distance or the highest or lowest point, whichever is shorter. (l) Western blotting analysis on purified exosomes from GW4896- and control-treated HeLa cells for CD63 and CD81.
Techniques Used: Expressing, Labeling, Imaging, Flow Cytometry, Immunostaining, Purification, Western Blot, Transfection, Fluorescence, Incubation, Activity Assay, Two Tailed Test
Figure Legend Snippet: CD63-pHluorin fusion events are derived from MVBs. (a) Left three panels: live imaging of fusion events (indicated by white arrows) over a time course of 12 s onto one cell before the event (left), at the start of the event (middle), and right before fixation of the cell (3). Right: inset showing a magnification of the localized sudden increase in fluorescence at the PM (highlighted by a dashed line square) right before fixation. (b) Left: correlation of light microscopy signal of a fusion event observed by live imaging with EM pictures of the first section of the cell facing the coverslip (low magnification). Right: correlation of light microscopy signal with the first slice of the electron tomographic reconstruction of the first section of the cell facing the coverslip. The orange circle indicates the error range (167 nm) of the correlation performed by eC-CLEM. (c) 3D model of the electron tomographic reconstruction. The ER is depicted in light violet. Dense compartments are depicted in brown. The structure of interest is depicted in red and orange. (d) Bottom side view of the 3D model of the compartment of interest in its surroundings. The white arrow indicates the opening of the MVB where ILVs are released. (e) 3D model showing the MVB isolated from its environment. ILVs secreted through the opening of the MVB are depicted in white. (f) Top view of the secretory profile of the MVB that correlates with the fluorescence burst of the CD63-pHluorin fusion event.
Techniques Used: Derivative Assay, Imaging, Fluorescence, Light Microscopy, Isolation
Figure Legend Snippet: MVB–PM fusion is distinct from other forms of vesicle-mediated exocytosis. (a) Schematic model showing the markers used in this study for the different types of cargo delivery of vesicles fusing with the PM. (b) Time-lapse imaging (heat maps) of a fusion event of the exosomal protein CD63-pHluorin. (c) Time-lapse images of soluble (NPY-pHluorin) and membrane protein (VAMP2-pHluorin) fusion events. (d) Fluorescent signal duration of NPY (mean = 0.85 s), VAMP2 (mean = 2.12 s), and CD63 (mean = 106.55 s) fusion events. n ≥ 13 events per reporter. (e) 3D heat maps of three consecutive CD63-pHluorin fusion event frames. (f) Western blot for exosomal markers (CD63 and Alix) on EVs purified from the supernatant (soluble) and EVs attached to the cell surface (PM attached) isolated after short trypsinization of the cells. (g) Direct comparison between signal duration of fusion events of CD81- and CD9-pHluorin relative to CD63-pHluorin. n ≥ 20 events per reporter. ***, P < 0.001; ****, P < 0.0001 using Student’s two-tailed two-sample t test.
Techniques Used: Imaging, Western Blot, Purification, Isolation, Two Tailed Test
Figure Legend Snippet: GPCR activation triggers MVB–PM fusion in single cells in a calcium-independent manner. (a) Schematic model of imaging setup. (b) Fusion activity of HeLa cells stimulated with KCl (70 mM), caffeine (20 mM), or histamine (100 µM). n ≥ 8 cells per condition. (c) Total projection of fusion events over a 60-s time course onto cells before (top) and after (bottom) stimulation with histamine (100 µM). Pseudocolored as in . (d) Measurement of individual HeLa cells ( n = 14) before and during stimulation with histamine (100 µM). (e) Mean fusion kinetics of CD63-pHluorin HeLa cells ( n = 6) showing the distribution of fusion events over time (dark blue line; SD is in light blue) and the calcium levels (red) during histamine stimulation (gray-shaded block). (f) Heat maps revealing calcium responses (measured by Fluo-4) upon histamine stimulation obtained by subtracting the fluorescent intensity values before stimulation from those after 8-s stimulation. Cells were nontreated or incubated with a buffer with fast (BAPTA) or slow (EGTA) calcium-binding kinetics. Bars, 10 µm. (g) Quantification of fusion activity of histamine-stimulated HeLa cells in the presence of EGTA (top) or BAPTA (bottom) buffers. n ≥ 10 cells per condition. (h) Measurement of individual HUVEC cells ( n = 30) before and after stimulation with histamine (100 µM). *, P < 0.05; **, P < 0.01 using Student’s two-tailed two-sample t test. All t tests were unpaired except for d and h. Whiskers in the box plots (b and g) represent 1.5 times the interquartile distance or the highest or lowest point, whichever is shorter.
Techniques Used: Activation Assay, Imaging, Activity Assay, Blocking Assay, Incubation, Binding Assay, Two Tailed Test
Figure Legend Snippet: The GPCR downstream effector SNAP23 regulates MVB–PM fusion. (a) Network of proteins of interest together with direct interactors with altered phosphorylation levels upon histamine (100 µM) stimulation as identified by phosphoproteomics in HeLa and HUVEC cells. Proteins of interest are depicted with a blue rim. FC, fold change. (b) Graph showing the signal intensity values of phosphorylated peptides from proteins of interest before and after stimulation with 100 µM histamine. Data represent means ± SD of two technical replicates per condition. *, P < 0.05 (P = 0.048) using Student’s two-tailed two-sample t test. (c) Western blotting analysis on SNAP23 protein expression in six different cell lines. (d) Confocal analysis of FL (GFP-SNAP23-FL) and truncated (GFP-SNAP23-CΔ9) GFP-SNAP23 (in gray)–transfected SiHa cells labeled for CD63 (red). (e) Total projection of fusion events in CD63-pHluorin SiHa cells cotransfected with SNAP23-FL or SNAP23-CΔ9 over 3 min. Pseudocolored as in . Bars, 10 µm. (f) Quantification of fusion events in CD63-pHluorin SiHa cells cotransfected with SNAP23-FL or SNAP23-CΔ9. n ≥ 10 cells per condition. (g) Confirmation of SNAP23 knockdown (KD) at the protein level in HeLa cells. (h) Effect of SNAP23 knockdown on MVB–PM fusion in HeLa cells. n ≥ 17 cells per condition. (i) Confirmation of SNAP23 and syntaxin-4 knockdown in HeLa cells at the mRNA level. Data represent means ± SD. (j) Effect of the knockdown of SNAP23 or syntaxin-4 on the fusion activity of HeLa cells. n ≥ 11 cells per condition. ctrl, nontransfected; siCTRL, control siRNA. *, P < 0.05; **, P < 0.01 using Student’s two-tailed two-sample t test. Whiskers in the box plots (f, h, and j) represent 1.5 times the interquartile distance or the highest or lowest point, whichever is shorter.
Techniques Used: Two Tailed Test, Western Blot, Expressing, Transfection, Labeling, Activity Assay
Figure Legend Snippet: GPCR activation triggers MVB–PM fusion in HeLa cells via SNAP23-Ser110 phosphorylation. (a) Fusion activity of histamine-stimulated cells nontreated or treated with the Gα q inhibitor UBO-QIC (1 µM). n ≥ 24 cells per condition. (b) Basal fusion activity in cells treated with PKC inhibitors GÖ6976 (1 µM) or GÖ6983 (1 µM). n ≥ 11 cells per condition. (c) Fusion activity of histamine-stimulated cells nontreated or preincubated with GÖ6983 (1 µM). n ≥ 11 cells per condition. (d) Schematic representation of SNAP23 with SNARE motifs, a membrane-anchoring domain (M), and all phosphosites with the posphosite targeted by histamine stimulation (Ser110) in bold. (e) Fusion activity of histamine-stimulated cells transfected with WT SNAP23, phosphomutant SNAP23-S110A, or phosphomimic SNAP23-S110D. n ≥ 16 cells per condition. (f) Left: fusion activity of CD63-, CD81-, and CD9-pHluorin HeLa cells cotransfected with SNAP23 WT or SNAP23-S110A. n ≥ 16 cells per condition. Western blot on exosomes isolated from SNAP23-WT and SNAP23-S110A HeLa cells labeled for CD63, CD9, CD81, flotillin-1, and syntenin-1. (g) Schematic representation of the histamine-stimulated pathway leading to exosome release as identified by phosphoproteomics and specific inhibitors. Blue-rimmed proteins represent the putative pathway implicated by both experiments. The IP3–Ca 2+ pathway is represented in gray as a direct link with MVB–PM fusion is missing. **, P < 0.01; ****, P < 0.0001 using Student’s two-tailed two-sample t test. All t tests were paired except for b and f. Whiskers in the box plots in b and f represent 1.5 times the interquartile distance or the highest or lowest point, whichever is shorter.
Techniques Used: Activation Assay, Activity Assay, Transfection, Western Blot, Isolation, Labeling, Two Tailed Test
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Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with Magnetic Beads:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with Transmission Assay:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with Electron Microscopy:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with Transfection:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with Injection:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with Enzyme-linked Immunosorbent Assay:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with Functional Assay:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with Infection:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with Enzyme-linked Immunospot:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with MANN-WHITNEY:Article Title: Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states Article Snippet: Sequencing libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (EpiCypher #14-1001) with no modifications. Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent. Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 ◦C with 20 μL of monoclonal antibodies(1:50): Article Title: Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients Article Snippet: Antibodies were purchased from the following vendors: mouse monoclonal antibody against CD63 (H5C6; #NBP2‐42225; RRID:AB_2884028) from Novus, against Flotillin‐1 (#610821; RRID:AB_398140) and Article Title: miR-331-depleted exosomes derived from injured endometrial epithelial cells promote macrophage activation during endometritis. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: A nanoparticle vaccine displaying varicella-zoster virus gE antigen induces a superior cellular immune response than a licensed vaccine in mice and non-human primates Article Snippet: Cells were further stained for 30 min with a mix of PerCP-conjugated CD3, BV421-conjugated anti-CD4 and Article Title: Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent Article Snippet: Exosomes were suspended in ice-cold PBS (108 particles/mL) and incubated for 2 h at 4 °C with 20 μL of monoclonal antibodies(1:50): Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2. Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment. Article Title: Biopotency and surrogate assays to validate the immunomodulatory potency of extracellular vesicles derived from mesenchymal stem/stromal cells for the treatment of experimental autoimmune uveitis Article Snippet: The expression levels of EV surface markers CD9, CD63 and CD81 were analysed by flow cytometry (CytoFLEX, Beckman coulter) using magnetic beads coated with anti‐CD63 (human CD63 Isolation/Detection kit; Invitrogen) with |