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cd63 (h5c6; biosciences  (Becton Dickinson)

 
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    Becton Dickinson cd63 (h5c6; biosciences
    Identification of endolysosomes in AP-5 patient lines. ( A ) Cryo-immunoelectron microscopy of patient-derived fibroblasts (p.Q578*) labelled with antibodies against the late endosomal/lysosomal markers, LAMP1, LBPA and <t>CD63.</t> Note that the enlarged endocytic structures label positive for all markers, with LAMP1 labelling restricted to the limiting membrane, and LBPA and <t>CD63</t> largely restricted to intraluminal whorls and striations. Scale bar = 200 nm. ( B ) Live imaging of control and patient-derived fibroblasts following incubation with Magic Red Cathepsin B reagent for 45 min at 37°C. Note that the enlarged structures in the patient lines are positive for cathepsin B activity and are therefore hydrolytically active. Scale bar = 20 µm. ( C ) Live imaging of control and patient-derived fibroblasts incubated with Lysotracker Red at 37°C. Note that the enlarged structures in the patient lines are positive for Lysotracker Red and are therefore acidic. Scale bar = 20 µm.
    Cd63 (H5c6; Biosciences, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+mouse+monoclonal+anti+cd63+dshb+h5c6/anti+cd63/pmc04527494-165-64-66
    Average 90 stars, based on 1 article reviews
    cd63 (h5c6; biosciences - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Loss of AP-5 results in accumulation of aberrant endolysosomes: defining a new type of lysosomal storage disease"

    Article Title: Loss of AP-5 results in accumulation of aberrant endolysosomes: defining a new type of lysosomal storage disease

    Journal: Human Molecular Genetics

    doi: 10.1093/hmg/ddv220

    Identification of endolysosomes in AP-5 patient lines. ( A ) Cryo-immunoelectron microscopy of patient-derived fibroblasts (p.Q578*) labelled with antibodies against the late endosomal/lysosomal markers, LAMP1, LBPA and CD63. Note that the enlarged endocytic structures label positive for all markers, with LAMP1 labelling restricted to the limiting membrane, and LBPA and CD63 largely restricted to intraluminal whorls and striations. Scale bar = 200 nm. ( B ) Live imaging of control and patient-derived fibroblasts following incubation with Magic Red Cathepsin B reagent for 45 min at 37°C. Note that the enlarged structures in the patient lines are positive for cathepsin B activity and are therefore hydrolytically active. Scale bar = 20 µm. ( C ) Live imaging of control and patient-derived fibroblasts incubated with Lysotracker Red at 37°C. Note that the enlarged structures in the patient lines are positive for Lysotracker Red and are therefore acidic. Scale bar = 20 µm.
    Figure Legend Snippet: Identification of endolysosomes in AP-5 patient lines. ( A ) Cryo-immunoelectron microscopy of patient-derived fibroblasts (p.Q578*) labelled with antibodies against the late endosomal/lysosomal markers, LAMP1, LBPA and CD63. Note that the enlarged endocytic structures label positive for all markers, with LAMP1 labelling restricted to the limiting membrane, and LBPA and CD63 largely restricted to intraluminal whorls and striations. Scale bar = 200 nm. ( B ) Live imaging of control and patient-derived fibroblasts following incubation with Magic Red Cathepsin B reagent for 45 min at 37°C. Note that the enlarged structures in the patient lines are positive for cathepsin B activity and are therefore hydrolytically active. Scale bar = 20 µm. ( C ) Live imaging of control and patient-derived fibroblasts incubated with Lysotracker Red at 37°C. Note that the enlarged structures in the patient lines are positive for Lysotracker Red and are therefore acidic. Scale bar = 20 µm.

    Techniques Used: Immuno-Electron Microscopy, Derivative Assay, Imaging, Incubation, Activity Assay

    Related Articles

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    Article Title: Microglia morphological response to mesenchymal stromal cell extracellular vesicles demonstrates EV therapeutic potential for modulating neuroinflammation
    Article Snippet: CD63 (1:2000, BDBiosciences, Cat#561983) antibody was incubated with MSC-EV samples for 2 h at room temperature (RT) prior to assessment.

    Article Title: Tumor-derived EV miRNA signatures surpass total EV miRNA in supplementing mammography for precision breast cancer diagnosis.
    Article Snippet: Subsequently, they were incubated with 5 μL of anti-CD63-PE-Cy7 antibodies (cat. 561982; Becton Dickinson), targeting the EV-specific marker, in the dark for 30 min at 4 °C for the relative quantification of BEVs.

    Article Title: Calpains Orchestrate Secretion of Annexin-containing Microvesicles during Membrane Repair
    Article Snippet: The resuspended high-speed pellet fraction and 1 μg anti-CD63 (clone H5C6, BD Biosciences), was added to the beads and incubated with rotation overnight at 4°C.

    Article Title: Calpains Orchestrate Secretion of Annexin-containing Microvesicles during Membrane Repair
    Article Snippet: Antibodies for immunoblot were: rabbit anti-vinculin (Abcam ab129002), rabbit anti-annexin A6 (Abcam 201024), rabbit anti-annexin A1 (Abcam ab214486), rabbit anti-annexin A2 (Abcam ab185957), rabbit anti-calpain 1 (Proteintech 10538-1-AP), mouse anti-CD63 (BD Biosciences 556019), rat anti-CD63 (LSBio LS-C179520), mouse anti-alix (Santa Cruz sc-53540), mouse anti-flotillin-2 (BD Biosciences 610384), rabbit anti-CD9 (CST D801A), mouse anti-tubulin (Abcam ab7291), rabbit anti-streptolysin O (GeneTex GTX64171), mouse anti-NanoLuc (R&D Systems MAB10026), rabbit anti-HA (CST C29F4), rabbit anti-LC3 (Novus NB100-2220), rabbit anti-Lamp1 (CST D2D11).

    Article Title: Tumor-derived EV miRNA signatures surpass total EV miRNA in supplementing mammography for precision breast cancer diagnosis.
    Article Snippet: For confocal microscopy, immunobeads bound to BEVs were incubated with 5 μL of anti-CD63-PE-Cy7 antibodies (cat. 561982; Becton Dickinson) for 1 h at room temperature in the dark to facilitate detection.

    Article Title: MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2.
    Article Snippet: Oral mucositis (OM) is a common condition affecting the oral mucosa of patients who undergo chemotherapy and/ or radiotherapy.. In head and neck cancer (HNC) patients receiving both chemotherapy and radiotherapy, the occurrence of OM can reach as high as 90%.1 Highgrade OM is not only painful but also significantly impacts the quality of life and prognosis of cancer patients.. Unfortunately, effective treatments for highgrade OM are lacking.2 Consequently, there is a need for novel therapies to enhance OM treatment.

    Article Title: Chaihu-Shugan-San alleviates post-stroke depression in mice: Mechanistic insights into exosome-mediated neuroprotection.
    Article Snippet: Ethnopharmacological relevance: Post-stroke depression (PSD) is common among stroke survivors and negatively impacts recovery.. Chaihu-Shugan-San (CSS), a traditional Chinese medicine, has shown therapeutic potential for mood disorders, particularly PSD.. Recent studies suggest that CSS’s effects may be mediated by exosomes, but the mechanisms remain unclear.

    Incubation:

    Article Title: Extracellular vesicles regulate metastable phenotypes of lymphangioleiomyomatosis cells via shuttling ATP synthesis to pseudopodia and activation of integrin adhesion complexes
    Article Snippet: .. Briefly, aldehyde/sulfate beads (Interfacial Dynamics, Grand Island, NY, USA) were incubated with capture human CD63 (BD Biosciences), human CD9 (Biolegend), and mouse CD9 (BioLegend, San Diego, CA, USA) antibodies and then with mouse plasma or conditioned media. .. EV-coated beads were incubated with Human CD63 (Biolegend), Human CD9 (Biolegend), Mouse CD63 (Biolegend), and Mouse CD9 (BD Biosciences) antibodies and analyzed by FACS.

    Clinical Proteomics:

    Article Title: Extracellular vesicles regulate metastable phenotypes of lymphangioleiomyomatosis cells via shuttling ATP synthesis to pseudopodia and activation of integrin adhesion complexes
    Article Snippet: .. Briefly, aldehyde/sulfate beads (Interfacial Dynamics, Grand Island, NY, USA) were incubated with capture human CD63 (BD Biosciences), human CD9 (Biolegend), and mouse CD9 (BioLegend, San Diego, CA, USA) antibodies and then with mouse plasma or conditioned media. .. EV-coated beads were incubated with Human CD63 (Biolegend), Human CD9 (Biolegend), Mouse CD63 (Biolegend), and Mouse CD9 (BD Biosciences) antibodies and analyzed by FACS.



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