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rabbit anti vdac1 2  (Proteintech)


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    Structured Review

    Proteintech rabbit anti vdac1 2
    Rabbit Anti Vdac1 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+vdac1+rabbit/VDAC1%2F2+Antibody/pmc12953705-61-0-12
    Average 95 stars, based on 173 article reviews
    rabbit anti vdac1 2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Ceritinib Induces Mitochondrial Fragmentation in Thyroid Cancer Cells by Targeting Drp-1.
    Article Snippet: Mitochondrial dynamics play a crucial role in thyroid cancer progression by regulating apoptosis, metabolism, and oxidative stress.. Ceritinib, a tyrosine kinase inhibitor, shows potential anticancer effects; however, its impact on mitochondrial function in thyroid cancer remains obscure.. Herein, we aim to investigate the impact of ceritinib on the mitochondrial functionality in TPC‐1 thyroid carcinoma cells and the underlying mechanism.

    Article Title: Aerobic exercise improve physical endurance and muscle function by ameliorating oxidative stress and modulating mitochondrial dynamics in aged skeletal muscle.
    Article Snippet: 1 College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China 2 College of Foreign Languages, Zhejiang Normal University, Jinhua 321004, China Abstract Mitochondrial dysfunction is associated with the age-related decline in skeletal muscle mass and strength.. Aerobic exercise upregulate Sestrins protein expression in skeletal muscle, which plays a key role in maintaining mitochondrial homeostasis.. This study aimed to elucidate the role of aerobic exercise in regulating mitochondrial dynamics and oxidative stress in aging skeletal muscle.

    Article Title: Modulating GRP75 to restore calcium homeostasis: a novel neuroprotective strategy of CRSE6 # in Parkinson's disease models.
    Article Snippet: The samples were separated by molecular weight using SDS-PAGE electrophoresis and transferred to PVDF (Cat#ISEQ00010-0.25, Sigma-Aldrich, Shanghai, China) membranes, and the non-specific sites were blocked with skimmed milk powder. .. Then incubated with the following primary antibodies at 4 °C: b-Actin Rabbit mAb (Cat#AC026, ABclonal, Wuhan, China), BAX Monoclonal antibody (Cat#60267-1-Ig, Proteintech, Wuhan, China), Bcl-2 Antibody (Cat#AF6139, Affinity, Jiangsu, China), Cytochrome c Polyclonal antibody (Cat#10093-1-AP, Proteintech, Wuhan, China), GRP78 Polyclonal antibody (Cat#11587-1-AP, Proteintech, Wuhan, China), GRP75 Polyclonal antibody (Cat#14887-1-AP, Proteintech, Wuhan, China), VDAC1 Monoclonal antibody (Cat#66345-1-AP, Proteintech, Wuhan, China), and IP3R-I (Cat#sc-271197, Santz Cruz, U.S.A.). ..

    Article Title: Disruption of sphingolipid metabolism triggers lung vascular inflammation and aging-like changes under hypoxia through VDAC1-mediated mitochondrial DNA release.
    Article Snippet: With the growing demand for travel and diverse occupational activities, people now have more opportunities to be exposed to high-altitude environments.. Low atmospheric pressure and hypobaric hypoxia pose significant health challenges to individuals in high-altitude areas [1].. Hypoxia is a primary driver of physiological and pathological changes [2].

    Immunostaining:

    Article Title: High-fat diet leads to male reproductive dysfunction by disrupting lipid-droplet-mediated organelle crosstalk.
    Article Snippet: .. The antibodies for immunostaining Antibody Source Identifier Dilution StAR GeneTex GTX636800 1:200 CYP11A1 GeneTex GTX56293 1:200 3β-HSD Santa Cruz sc-515120 1:50 SYCP3 Santa Cruz sc-74569 1:50 CREM GeneTex GTX114146 1:100 VDAC1 Proteintech 81538-1-RR 1: 500 α-Tubulin Abcam ab52866 1: 200 Nestin Millipore MAB353 1: 200 G-Actin Proteintech 11227-1-AP 1:300 Tom20 Proteintech 11802-1-AP 1: 200 PDI Proteintech 11245-1-AP 1: 200 COX2 Proteintech 27308-1-AP 1: 200 Goat Anti-rabbit IgG Alexa 488 Invitrogen A-11008 1:500 Goat Anti-mouse IgG Alex 488 Invitrogen A- 32723 1:500 Goat Anti-mouse IgG Alexa 555 Invitrogen A-31572 1:500 Goat Anti-rabbit IgG Alexa 647 Invitrogen A- 31573 1:500 Goat Anti-mouse IgG Alex 647 Invitrogen A-21235 1:500 ..

    Saline:

    Article Title: Aerobic exercise improve physical endurance and muscle function by ameliorating oxidative stress and modulating mitochondrial dynamics in aged skeletal muscle.
    Article Snippet: 1 College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China 2 College of Foreign Languages, Zhejiang Normal University, Jinhua 321004, China Abstract Mitochondrial dysfunction is associated with the age-related decline in skeletal muscle mass and strength.. Aerobic exercise upregulate Sestrins protein expression in skeletal muscle, which plays a key role in maintaining mitochondrial homeostasis.. This study aimed to elucidate the role of aerobic exercise in regulating mitochondrial dynamics and oxidative stress in aging skeletal muscle.

    Pyrolysis Gas Chromatography:

    Article Title: Aerobic exercise improve physical endurance and muscle function by ameliorating oxidative stress and modulating mitochondrial dynamics in aged skeletal muscle.
    Article Snippet: 1 College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China 2 College of Foreign Languages, Zhejiang Normal University, Jinhua 321004, China Abstract Mitochondrial dysfunction is associated with the age-related decline in skeletal muscle mass and strength.. Aerobic exercise upregulate Sestrins protein expression in skeletal muscle, which plays a key role in maintaining mitochondrial homeostasis.. This study aimed to elucidate the role of aerobic exercise in regulating mitochondrial dynamics and oxidative stress in aging skeletal muscle.



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    ( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and <t>VDAC1</t> proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies <t>(rabbit</t> <t>anti-VDAC1</t> and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .
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    ( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and <t>VDAC1</t> proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies <t>(rabbit</t> <t>anti-VDAC1</t> and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .
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    ( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and <t>VDAC1</t> proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies <t>(rabbit</t> <t>anti-VDAC1</t> and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .
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    ( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and <t>VDAC1</t> proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies <t>(rabbit</t> <t>anti-VDAC1</t> and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .
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    Image Search Results


    ( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and VDAC1 proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies (rabbit anti-VDAC1 and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .

    Journal: The EMBO Journal

    Article Title: STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria

    doi: 10.1038/s44318-026-00700-8

    Figure Lengend Snippet: ( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and VDAC1 proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies (rabbit anti-VDAC1 and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .

    Article Snippet: Rabbit anti-VDAC1 (1:300 in blocking buffer, for IF) , Proteintech , 55259-1-AP.

    Techniques: Fractionation, Western Blot, Control, Incubation