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Structured Review

Taiclone Biotech Corporation rabbit anti-vdac1
Pathway enrichment.
Rabbit Anti Vdac1, supplied by Taiclone Biotech Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vdac1+rabbit/pmc07465595-77-124-127?v=Taiclone+Biotech+Corporation
Average 90 stars, based on 1 article reviews
rabbit anti-vdac1 - by Bioz Stars, 2026-08
90/100 stars

Images

1) Product Images from "Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells"

Article Title: Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells

Journal: Brain Sciences

doi: 10.3390/brainsci10080545

Pathway enrichment.
Figure Legend Snippet: Pathway enrichment.

Techniques Used: Expressing

Immunoblotting analysis to validate the differential expression for 9 proteins in VPA-treated and PBS-treated biological replicated SH-SY5Y cells. ( A ) Immunoblotting showed the expression of ATP5J, FBXO4, HDAC1, HDAC2, SMARCA4, UBE1L, UBE2D1, UQCRB, and VDAC17 in VPA-treated and PBS-treated SH-SY5Y cells. ( B ) Quantification showing the fold differences in the expression of ATP5J, FBXO4, HDAC1, HDAC2, SMARCA4, UBE2D1, UQCRB, and VDAC1 between VPA-treated groups and PBS control. GAPDH was a loading control. The data are expressed as fold change to PBS ± SD (* p < 0.05, n = 3 to 6).
Figure Legend Snippet: Immunoblotting analysis to validate the differential expression for 9 proteins in VPA-treated and PBS-treated biological replicated SH-SY5Y cells. ( A ) Immunoblotting showed the expression of ATP5J, FBXO4, HDAC1, HDAC2, SMARCA4, UBE1L, UBE2D1, UQCRB, and VDAC17 in VPA-treated and PBS-treated SH-SY5Y cells. ( B ) Quantification showing the fold differences in the expression of ATP5J, FBXO4, HDAC1, HDAC2, SMARCA4, UBE2D1, UQCRB, and VDAC1 between VPA-treated groups and PBS control. GAPDH was a loading control. The data are expressed as fold change to PBS ± SD (* p < 0.05, n = 3 to 6).

Techniques Used: Western Blot, Expressing

RT-qPCR assay showing the expression of 14 genes ( ATP5F1A , ATP5PF , BAZ1B , BMS1 , FBXO2 , HDAC1 , HDAC2 , SDHA , SMARCA4 , UBA7 , UBE2D1 , UQCRB , VDAC1 , and WDR36 ) in VPA-treated and PBS-treated SH-SY5Y cells. The GAPDH gene was used as the endogenous gene for normalization. The data are expressed as fold change to PBS ± SD (* p < 0.05, n = 3 to 6).
Figure Legend Snippet: RT-qPCR assay showing the expression of 14 genes ( ATP5F1A , ATP5PF , BAZ1B , BMS1 , FBXO2 , HDAC1 , HDAC2 , SDHA , SMARCA4 , UBA7 , UBE2D1 , UQCRB , VDAC1 , and WDR36 ) in VPA-treated and PBS-treated SH-SY5Y cells. The GAPDH gene was used as the endogenous gene for normalization. The data are expressed as fold change to PBS ± SD (* p < 0.05, n = 3 to 6).

Techniques Used: Quantitative RT-PCR, Expressing



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Image Search Results


( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and VDAC1 proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies (rabbit anti-VDAC1 and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .

Journal: The EMBO Journal

Article Title: STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria

doi: 10.1038/s44318-026-00700-8

Figure Lengend Snippet: ( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and VDAC1 proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies (rabbit anti-VDAC1 and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .

Article Snippet: Rabbit anti-VDAC1 (1:300 in blocking buffer, for IF) , Proteintech , 55259-1-AP.

Techniques: Fractionation, Western Blot, Control, Incubation