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anti vdac1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti vdac1
Anti Vdac1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vdac1+rabbit/VDAC+Rabbit+mAb/pm41688443-99-49-50
Average 96 stars, based on 523 article reviews

anti vdac1 - by Bioz Stars, 2026-09

96/100 stars

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Membrane:

Article Title: Antibodies having specificity to HER4 and uses thereof
Article Snippet: .. Membranes were further probed with anti-HER4 antibody E200 (Abcam), which identifies the whole HER4 receptor and its IntraCellular Domain 4ICD, and with anti-aTubulin (ref. 3873S, CST), anti-VDCA1 (Voltage-Dependent Anion-selective Channel 1; ref. 4661S, CST) or −TIM23 (Mitochondrial Import Inner Membrane translocase subunit; Becton Dickinson, ref. 611223), and anti-Histone H3 (ref. 9715S, CST) antibodies which identify the cytosolic, mitochondrial and nuclear fractions respectively. .. After three washes in TNT, blots were incubated with peroxidase-conjugated mouse-specific (1/10000) or rabbit-specific (1/10000) antibodies (Sigma) as appropriate, for 1 h in TNT-2% nonfat dry milk at ambient temperature.

Article Title: Chronic cold exposure induces plasticity of mitochondrial calcium uptake in beige and brown fat of UCP1-deficient mice
Article Snippet: .. Lysates were resolved by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE), transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore), and probed with antibodies against Na + /K + -ATPase (Abcam, ab76020, GR3237646-13, 1/10,000), TOMM20 (Sigma Prestige, HPA011562, 1/500), MCU (Cell Signaling Technology, 14997, 1/100), UCP1 (Abcam, ab10983, 1/1000), NCLX (Proteintech, 21430-1-AP, 1/500), TMBIM5 (Proteintech, 16296-1-AP, 1/500), TMEM65 (Invitrogen, PA5-112762, 1/500), GRP75 (Abcam, ab53098, 1/500), Calnexin (BD Transduction Laboratories, 610523, 1/1000), VDAC (Cell Signaling Technology, 4661, 1/2000), CyD (Proteintech, 18466-1-AP, 1/1000), OXPHOS (Abcam, ab110413, 1/500), Vinculin (1:10000; V9131; Sigma-Aldrich), NDUFB6 (1:5000; #6510832 Thermo Fisher Scientific), mtCOI (1:10000; #10444035; Thermo Fisher Scientific), ATP5A (1:1,000; #439800; Thermo Fisher Scientific), SDHA (1:1,000, #459200, Thermo Fisher Scientific), SDHB (1:1,000, #ab14714, Abcam), UQCRC2 (1:1,000, #14742-1-AP; Proteintech), ATPIF1 (1:1,000, #13268S; Cell Signaling Technology), TOMM20 Alexa Fluor 488 (1:1,000; #ab205486; Abcam), and cytochrome c (1:5,000, #ab110325, Abcam). ..

Western Blot:

Article Title: Male obesity causes adipose mitochondrial dysfunction in F 1 mouse progeny via a let-7-DICER axis
Article Snippet: After being washed once by PBS, the treated cells at harvest time were lysed by RIPA buffer supplemented with cOmplete and PhosSTOP and quantified by Pierce BCA Protein Assay Kit (23227, Thermo Fisher). .. Western blot analyses were carried out according to standard protocols with primary antibodies against COXIV (Cell Signaling Technologies #4850, 1:11,000 dilution), VDAC (Cell Signaling Technologies #4661, 1:11,000 dilution), HK2 (Cell Signaling Technologies #2897, 1:1,1000 dilution), SLC25A24 (Abcam Ab221120 , 1:1000 dilution), CPT2 (Abcam Ab181114 , 1:1,1000 dilution), CRAT (AP Proteintech #15170, 1:1000) and IDH2 (Cell Signaling Technologies #60322, 1:1,1000 dilution). .. Relative protein quantification was normalized by HSC70 (Santa Cruz sc-7298, 1:1000 dilution) or HSP90 (Cell Signaling Technologies #4877, 1:1000 dilution).

Article Title: Male obesity causes adipose mitochondrial dysfunction in F 1 mouse progeny via a let-7-DICER axis.
Article Snippet: Protein extraction and western blot analysis: After being washed once by phosphate-buffered saline (PBS), the treated cells at harvest time were lysed by RIPA buffer supplemented with cOmplete and PhosSTOP and quantified by Pierce BCA Protein Assay Kit (23227, Thermo Fisher). .. Western blot analyses were carried out according to standard protocols with primary antibodies against COXIV (Cell Signaling Technologies #4850, 1:1,1000 dilution), VDAC (Cell Signaling Technologies #4661, 1:1,1000 dilution), HK2 (Cell Signaling Technologies #2897, 1:1,1000 dilution), SLC25A24 (Abcam Ab221120, 1:1000 dilution), CPT2 (Abcam Ab181114, 1:1,1000 dilution), CRAT (AP Proteintech #15170, 1:1,000) and IDH2 (Cell Signaling Technologies #60322, 1:1,1000 dilution). .. Relative protein quantification was normalized by HSC70 (Santa Cruz sc-7298, 1:1000 dilution) or HSP90 (Cell Signaling Technologies #4877, 1:1000 dilution).

Nucleic Acid Electrophoresis:

Polyacrylamide Gel Electrophoresis:

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$( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and VDAC1 proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies (rabbit anti-VDAC1 and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .$

Journal: The EMBO Journal

Article Title: STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria

doi: 10.1038/s44318-026-00700-8

Figure Lengend Snippet: ( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and VDAC1 proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies (rabbit anti-VDAC1 and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .

Article Snippet: Rabbit anti-VDAC1 (1:300 in blocking buffer, for IF) , Proteintech , 55259-1-AP.

Techniques: Fractionation, Western Blot, Control, Incubation