rabbit anti vdac1 (Proteintech)

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Proteintech rabbit anti vdac1
$( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and <t>VDAC1</t> proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies <t>(rabbit</t> <t>anti-VDAC1</t> and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .$
Rabbit Anti Vdac1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vdac1+rabbit/VDAC1%2FPorin+Antibody/pmc12953705-60-0-9
Average 96 stars, based on 270 article reviews

rabbit anti vdac1 - by Bioz Stars, 2026-10

96/100 stars

Images

1) Product Images from "STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria"

Article Title: STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria

Journal: The EMBO Journal

doi: 10.1038/s44318-026-00700-8

$( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and VDAC1 proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies (rabbit anti-VDAC1 and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .$
Figure Legend Snippet: ( A ) Wild-type HEK293 and STIM1-KO cells were subjected to MAM fractionation, and TH, CM, MAM, and ER fractions were analyzed by immunoblotting (5 μg protein/lane). ACSL4 was used as a loading control for MAM fraction. ( B – D ) Enrichment of IP3Rs in the MAM fraction was quantified relative to total ER levels (MAM + ER signals). Data were normalized to the WT condition and plotted as mean ± S.D. from n = 5 biological replicates for IP3R3 and IP3R2, and n = 3 biological replicates for IP3R1/2/3. Statistical analysis with unpaired t-test in all cases. ( E ) GRP75 and VDAC1 proteins levels were analyzed in the MAM fractions from WT HEK293 and STIM1-KO HEK293 cells. ACSL4 was used as a loading control. ( F , G ) Data were normalized to the WT condition and plotted as mean ± S.D. from n = 4 biological replicates. Statistical analysis with unpaired t-test, p = 0.0288 in ( F ), p = 0.0452 in ( G ). ( H ) Methanol-fixed HEK293 cells were incubated with the indicated primary antibodies (rabbit anti-VDAC1 and/or mouse anti-IP3R1/2/3) as well as the DNA probes rabbit-PLUS and mouse-MINUS. As negative controls, cells were incubated with individual primary antibodies. Representative images for all the conditions are shown. Scale bar = 10 μm. ( I ) Quantification of the assay from ( H ), with the number of cells analyzed indicated in parentheses. The black line represents the mean of the data. Statistical analysis with unpaired t-test, **** p < 0.0001, * p = 0.0139. .

Techniques Used: Fractionation, Western Blot, Control, Incubation

Western Blot:

Article Title: Parkinson's disease-associated PLA2G6 protects IP3R1 protein to control ER-mitochondria tethering and Ca 2+ transfer.
Article Snippet: Immunoblotting signals were detected using a chemiluminescence reagent (Invitrogen, 32106) and quantified via ImageJ. .. The antibodies used for immunoblotting included PLA2G6 (Abcam, ab259950, 1:1000), GRP75 (Santa Cruz, sc-133137, 1:500), VDAC1 (Proteintech, 55259-1-AP, 1:1000), Sigma1R (CST, 61994, 1:1000), COX IV (ABclonal, A11631, 1:1000), Calreticulin (ABclonal, A20986, 1:1000), IP3R1 (ABclonal, A21471, 1:1000), MFN2 (Abcam, ab124773, 1:1000), TOM40 (ABclonal, A3213, 1:1000), TOM20 (CST, 42406S, AR TI CL E IN P RE SS 1:1000), TIM23 (Proteintech, 11123-1-AP, 1:1000), ubiquitin (Santa Cruz, sc-8017, 1:500), ERLIN2 (ABclonal, A0781, 1:1000), NDUFA9 (ABclonal, A3196, 1:1000), pS129 (CST, 23706, 1:1000), GSK3β (ABclonal, A2081, 1:1000), DJ-1 (Proteintech, 68915-6-Ig, 1:1000), GFP (HUABIO, ET1607-31, 1:2000), LAMP1 (Santa Cruz, sc-20011, 1:500) and GAPDH (Proteintech, 60004-1-Ig, 1:5000). .. For Co-IP assays, proteins were extracted from cells and mouse ventral midbrains using 1% NP-40 lysis buffer (Beyotime, P0013F) supplemented with a protease inhibitor cocktail (MCE, HY-K0010) on ice for 30 minutes.

Ubiquitin Proteomics:

Incubation:

Article Title: Hematopoietic Cell Kinase Promotes Cerebral Ischemic Injury and Mitochondrial Dysfunction via p38 Activation in Ischemic Stroke.
Article Snippet: Ischemic stroke is a leading cause of death and permanent disability worldwide.. There is an urgent need to identify novel therapeutic targets.. Through bioinformatics analysis of Gene Expression Omnibus (GEO) datasets and the construction of protein–protein interaction (PPI) network, we identified hematopoietic cell kinase (HCK) as a key regulatory factor in ischemic stroke.

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