colloidal gold conjugated donkey ant sheep igg secondary antibody (Jackson Immuno)
Jackson Immuno is a verified supplier 
93
Structured Review
Jackson Immuno
colloidal gold conjugated donkey ant sheep igg secondary antibody
Colloidal Gold Conjugated Donkey Ant Sheep Igg Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ant-185/Colloidal+Gold+AffiniPure+Donkey+Anti-Sheep+IgG/pm25707796-417-8-18
Average 93 stars, based on 7 article reviews
Colloidal Gold Conjugated Donkey Ant Sheep Igg Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ant-185/Colloidal+Gold+AffiniPure+Donkey+Anti-Sheep+IgG/pm25707796-417-8-18
Average 93 stars, based on 7 article reviews
colloidal gold conjugated donkey ant sheep igg secondary antibody - by Bioz Stars,
2026-09
93/100 stars
Images
Related Articles
Purification:Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Viral particles were examined under a H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. .. For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for one hour and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4°C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Transmission electron microscopy Lentiviral particles were placed on a Formvar Carbon Film (400 mesh, copper, Electron Microscopy Sciences) and processed for electron microscopy by staining with 1% ammonium molybdate. .. Viral particles were examined under a H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. . Immunogold electron microscopy For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for one hour and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4°C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery. Article Snippet: Viral particles were examined under an H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. .. For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for 1 h and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4 C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Blocking Assay:Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Viral particles were examined under a H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. .. For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for one hour and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4°C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Transmission electron microscopy Lentiviral particles were placed on a Formvar Carbon Film (400 mesh, copper, Electron Microscopy Sciences) and processed for electron microscopy by staining with 1% ammonium molybdate. .. Viral particles were examined under a H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. . Immunogold electron microscopy For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for one hour and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4°C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery. Article Snippet: Viral particles were examined under an H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. .. For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for 1 h and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4 C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Incubation:Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Viral particles were examined under a H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. .. For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for one hour and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4°C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Article Title: Activation of STING requires palmitoylation at the Golgi Article Snippet: They were incubated with 1% BSA and PBS for 20 min at room temperature, and then with two primary antibodies, anti-p-TBK1 (1:50, D52C2) and anti-TGN46 (1:200, AHP500GT, Bio-Rad), for 3 days at 4 °C. .. After incubation with two CG-conjugated secondary antibodies, 6 nm Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Transmission electron microscopy Lentiviral particles were placed on a Formvar Carbon Film (400 mesh, copper, Electron Microscopy Sciences) and processed for electron microscopy by staining with 1% ammonium molybdate. .. Viral particles were examined under a H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. . Immunogold electron microscopy For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for one hour and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4°C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery. Article Snippet: Viral particles were examined under an H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. .. For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for 1 h and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4 C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Article Title: Hypothalamic POMC neurons promote cannabinoid-induced feeding. Article Snippet: After overnight incubation of primary antibodies (see Supplementary Table 1), sections were washed, incubated with biotin-conjugated donkey anti-rabbit IgG secondary antibody (dilution 1:250, 711-065-152, Jackson ImmunoResearch Laboratories) for 2 h, washed again, put in avidin–biotin complex (ABC; Vector Laboratories), and developed with 3,3-diaminobenzidine (DAB). .. After washing, sections were incubated in 6 nm Western Blot:Article Title: Activation of STING requires palmitoylation at the Golgi Article Snippet: .. Other antibodies used in this study were as follows: mouse anti-GFP (JL-8, dilution 1:1,000; Clontech); mouse anti-GFP (3E6, dilution 1:500), Alexa 488-, 594- or 647-conjugated secondary antibodies (A21202, A21203, A21206, A21207, A31573, A11016, A21448, dilution 1:2,000; Life Technologies); rabbit anti-TBK1 (ab40676, dilution 1:1,000; Abcam); rabbit anti-phospho-TBK1 (D52C2, dilution 1:1,000 for western blotting, dilution 1:100 for immunofluorescence, dilution 1:50 for immunoelectron microscopy), rabbit anti-phospho-IRF3 (4D4G, dilution 1:1,000), rabbit anti-p65 (D14E12, dilution 1:1,000), and rabbit anti-phospho-p65 (93H1, dilution 1:1,000; Cell Signalling); rabbit anti-IRF3 (FL-425, dilution 1:200; Santa Cruz); rabbit anti-Rab11 (71-5300, dilution 1:100; Zymed); mouse anti-p62 (610382, dilution 1:1,000), mouse anti-calreticulin (612136, dilution 1:200) and mouse anti-GM130 (610823, dilution 1:1,000) (BD Biosciences); mouse anti-α-tubulin (DM1A, dilution 1:5,000; Sigma); sheep anti-mouse IgG antibody-HRP (NA9310V, dilution 1:4,000) and donkey anti-rabbit IgG antibody-HRP (NA9340V, dilution 1:4,000) (GE Healthcare); sheep anti-TGN46 (AHP500G, dilution 1:4,000 for immunofluorescence, dilution 1:200 for immunoelectron microscopy) and sheep anti-TGN38 (AHR499G, dilution 1:200) (Serotec); rabbit anti-STING antibody (19851-1-AP, dilution 1:1,000 for western blotting; Proteintech); rabbit anti-syntaxin5 (110,053, dilution 1:200; Synaptic Systems); mouse anti-HA (4B2, dilution 1:1,000 for western blotting and immunofluorescence; Wako); donkey anti-mouse IgG (H+L) conjugated with DyLight 405 (715-475-150, dilution 1:2,000), colloidal gold (CG) particle-conjugated donkey anti-rabbit antibody (12 nm; 711-205-152, dilution 1:20) and Immunofluorescence:Article Title: Activation of STING requires palmitoylation at the Golgi Article Snippet: .. Other antibodies used in this study were as follows: mouse anti-GFP (JL-8, dilution 1:1,000; Clontech); mouse anti-GFP (3E6, dilution 1:500), Alexa 488-, 594- or 647-conjugated secondary antibodies (A21202, A21203, A21206, A21207, A31573, A11016, A21448, dilution 1:2,000; Life Technologies); rabbit anti-TBK1 (ab40676, dilution 1:1,000; Abcam); rabbit anti-phospho-TBK1 (D52C2, dilution 1:1,000 for western blotting, dilution 1:100 for immunofluorescence, dilution 1:50 for immunoelectron microscopy), rabbit anti-phospho-IRF3 (4D4G, dilution 1:1,000), rabbit anti-p65 (D14E12, dilution 1:1,000), and rabbit anti-phospho-p65 (93H1, dilution 1:1,000; Cell Signalling); rabbit anti-IRF3 (FL-425, dilution 1:200; Santa Cruz); rabbit anti-Rab11 (71-5300, dilution 1:100; Zymed); mouse anti-p62 (610382, dilution 1:1,000), mouse anti-calreticulin (612136, dilution 1:200) and mouse anti-GM130 (610823, dilution 1:1,000) (BD Biosciences); mouse anti-α-tubulin (DM1A, dilution 1:5,000; Sigma); sheep anti-mouse IgG antibody-HRP (NA9310V, dilution 1:4,000) and donkey anti-rabbit IgG antibody-HRP (NA9340V, dilution 1:4,000) (GE Healthcare); sheep anti-TGN46 (AHP500G, dilution 1:4,000 for immunofluorescence, dilution 1:200 for immunoelectron microscopy) and sheep anti-TGN38 (AHR499G, dilution 1:200) (Serotec); rabbit anti-STING antibody (19851-1-AP, dilution 1:1,000 for western blotting; Proteintech); rabbit anti-syntaxin5 (110,053, dilution 1:200; Synaptic Systems); mouse anti-HA (4B2, dilution 1:1,000 for western blotting and immunofluorescence; Wako); donkey anti-mouse IgG (H+L) conjugated with DyLight 405 (715-475-150, dilution 1:2,000), colloidal gold (CG) particle-conjugated donkey anti-rabbit antibody (12 nm; 711-205-152, dilution 1:20) and Immuno-Electron Microscopy:Article Title: Activation of STING requires palmitoylation at the Golgi Article Snippet: .. Other antibodies used in this study were as follows: mouse anti-GFP (JL-8, dilution 1:1,000; Clontech); mouse anti-GFP (3E6, dilution 1:500), Alexa 488-, 594- or 647-conjugated secondary antibodies (A21202, A21203, A21206, A21207, A31573, A11016, A21448, dilution 1:2,000; Life Technologies); rabbit anti-TBK1 (ab40676, dilution 1:1,000; Abcam); rabbit anti-phospho-TBK1 (D52C2, dilution 1:1,000 for western blotting, dilution 1:100 for immunofluorescence, dilution 1:50 for immunoelectron microscopy), rabbit anti-phospho-IRF3 (4D4G, dilution 1:1,000), rabbit anti-p65 (D14E12, dilution 1:1,000), and rabbit anti-phospho-p65 (93H1, dilution 1:1,000; Cell Signalling); rabbit anti-IRF3 (FL-425, dilution 1:200; Santa Cruz); rabbit anti-Rab11 (71-5300, dilution 1:100; Zymed); mouse anti-p62 (610382, dilution 1:1,000), mouse anti-calreticulin (612136, dilution 1:200) and mouse anti-GM130 (610823, dilution 1:1,000) (BD Biosciences); mouse anti-α-tubulin (DM1A, dilution 1:5,000; Sigma); sheep anti-mouse IgG antibody-HRP (NA9310V, dilution 1:4,000) and donkey anti-rabbit IgG antibody-HRP (NA9340V, dilution 1:4,000) (GE Healthcare); sheep anti-TGN46 (AHP500G, dilution 1:4,000 for immunofluorescence, dilution 1:200 for immunoelectron microscopy) and sheep anti-TGN38 (AHR499G, dilution 1:200) (Serotec); rabbit anti-STING antibody (19851-1-AP, dilution 1:1,000 for western blotting; Proteintech); rabbit anti-syntaxin5 (110,053, dilution 1:200; Synaptic Systems); mouse anti-HA (4B2, dilution 1:1,000 for western blotting and immunofluorescence; Wako); donkey anti-mouse IgG (H+L) conjugated with DyLight 405 (715-475-150, dilution 1:2,000), colloidal gold (CG) particle-conjugated donkey anti-rabbit antibody (12 nm; 711-205-152, dilution 1:20) and Transmission Assay:Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Transmission electron microscopy Lentiviral particles were placed on a Formvar Carbon Film (400 mesh, copper, Electron Microscopy Sciences) and processed for electron microscopy by staining with 1% ammonium molybdate. .. Viral particles were examined under a H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. . Immunogold electron microscopy For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for one hour and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4°C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Microscopy:Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Transmission electron microscopy Lentiviral particles were placed on a Formvar Carbon Film (400 mesh, copper, Electron Microscopy Sciences) and processed for electron microscopy by staining with 1% ammonium molybdate. .. Viral particles were examined under a H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. . Immunogold electron microscopy For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for one hour and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4°C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), Electron Microscopy:Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Transmission electron microscopy Lentiviral particles were placed on a Formvar Carbon Film (400 mesh, copper, Electron Microscopy Sciences) and processed for electron microscopy by staining with 1% ammonium molybdate. .. Viral particles were examined under a H-7650 Transmission Electron Microscope (Hitachi) at 80 kV. . Immunogold electron microscopy For detection of Myomaker, Myomerger, and VSV-G on lentiviral particles, virions purified by ultracentrifugation and a sucrose cushion were placed on a Formvar Carbon Film as described above, followed by blocking with 1% BSA for one hour and incubation in primary antibodies targeting Myomaker (1:10, # SC-244459, Santa Cruz), Myomerger (1:20, #AF4580, R&D), or VSV-G (1:20, #8G5F11, Kerafast) in 1% BSA overnight at 4°C followed by gold-conjugated secondary antibodies against goat (12 nm, 1:20, #705-205-147, Jackson ImmunoResearch), other:Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery Article Snippet: Donkey anti-Sheep IgG (H+L) (EM Grade), 6nm Colloidal Gold AffiniPure , Jackson ImmunoResearch , Cat #713-195-147. |