antineuroligin2 antibody (Alomone Labs)
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antineuroligin2 antibody
Antineuroligin2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anr-036/Anti-Neuroligin+2+(extracellular)+Antibody/pm32641624-57-43-51
Average 90 stars, based on 4 article reviews
Antineuroligin2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anr-036/Anti-Neuroligin+2+(extracellular)+Antibody/pm32641624-57-43-51
Average 90 stars, based on 4 article reviews
antineuroligin2 antibody - by Bioz Stars,
2026-09
90/100 stars
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other:Article Title: A critical role of Neuroligin 2 C-terminus in OCD and social behavior Article Snippet: Neurodevelopmental and neuropsychiatric disorders such as autism and schizophrenia are devastating brain illnesses that are often associated with deficits in social behaviors.. Thus, understanding the molecular mechanisms contributing to the etiology underlying these social defects will be critical in developing therapeutic strategies for these disorders.. Here, we have identified a crucial role of carboxyl-terminus (C-tail) of Neuroligin 2 (NL2), a cell adhesion molecule highly enriched at inhibitory synapses, in social behaviors. Labeling:Article Title: SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Article Snippet: .. For surface labeling, cells were incubated in block solution (PBS, 10% horse serum, 0.5%BSA) for 10mins, and then stained for 1h at RT with primary antibody diluted in Incubation:Article Title: SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Article Snippet: .. For surface labeling, cells were incubated in block solution (PBS, 10% horse serum, 0.5%BSA) for 10mins, and then stained for 1h at RT with primary antibody diluted in Article Title: SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Article Snippet: A whole brain, excluding the cerebellum, was homogenized on ice in lysis buffer (50mMHEPES pH 7.5, 0.5%Triton X-100, 150mMNaCl, 1mMEDTA, 1mMPMSFwith antipain, pepstatin and leupeptin at 10 mg/ml) and then left to rotate for 1h at 4 C. Membranes were pelleted by ultracentrifugation at 38,000xg for Cell Reports 29, 2599–2607.e1–e6, November 26, 2019 e4 40 mins at 4 C. Protein content of the supernatant was assayed by BioRad protein assay. .. For co-immunoprecipitations from brain lysate, 4 mg of brain lysate was incubated overnight at 4 Cwith rotation with 1 mg Rabbit IgG (ThermoFisher, 10500C) or Blocking Assay:Article Title: SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Article Snippet: .. For surface labeling, cells were incubated in block solution (PBS, 10% horse serum, 0.5%BSA) for 10mins, and then stained for 1h at RT with primary antibody diluted in Article Title: Phosphorylation of neuroligin-2 by PKA regulates its cell surface abundance and synaptic stabilization. Article Snippet: The trans-synaptic adhesion molecule neuroligin-2 (NL2) is essential for the development and function of inhibitory synapses.. NL2 recruits the postsynaptic scaffold protein gephyrin, which, in turn, stabilizes -aminobutyric acid type A receptors (GABAARs) in the postsynaptic domain.. Thus, the amount of NL2 at the synapse can control synaptic GABAAR concentration to tune inhibitory neurotransmission efficacy. Staining:Article Title: SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Article Snippet: .. For surface labeling, cells were incubated in block solution (PBS, 10% horse serum, 0.5%BSA) for 10mins, and then stained for 1h at RT with primary antibody diluted in Article Title: Phosphorylation of neuroligin-2 by PKA regulates its cell surface abundance and synaptic stabilization. Article Snippet: The trans-synaptic adhesion molecule neuroligin-2 (NL2) is essential for the development and function of inhibitory synapses.. NL2 recruits the postsynaptic scaffold protein gephyrin, which, in turn, stabilizes -aminobutyric acid type A receptors (GABAARs) in the postsynaptic domain.. Thus, the amount of NL2 at the synapse can control synaptic GABAAR concentration to tune inhibitory neurotransmission efficacy. Binding Assay:Article Title: Prickle2 and Igsf9b Coordinately Regulate the Cytoarchitecture of the Axon Initial Segment. Article Snippet: .. To validate the binding partners of Prickle2 in the immunoprecipitation assay, we used the following specific antibodies: anti-Prickle2 antibody (pAb, homemade, Hida et al., 2011), anti-Igsf9b antibody (pAb, Cat#: HPA010802, RRID:AB_1079194, Sigma-Aldrich), anti-Igsf9b antibody (clone 993107, Cat#: MAP9140, R&D Systems, MN, USA), and Immunoprecipitation:Article Title: Prickle2 and Igsf9b Coordinately Regulate the Cytoarchitecture of the Axon Initial Segment. Article Snippet: .. To validate the binding partners of Prickle2 in the immunoprecipitation assay, we used the following specific antibodies: anti-Prickle2 antibody (pAb, homemade, Hida et al., 2011), anti-Igsf9b antibody (pAb, Cat#: HPA010802, RRID:AB_1079194, Sigma-Aldrich), anti-Igsf9b antibody (clone 993107, Cat#: MAP9140, R&D Systems, MN, USA), and |