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annexin v fitc propidium iodide pi pe apoptosis detection kit  (MedChemExpress)


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    MedChemExpress annexin v fitc propidium iodide pi pe apoptosis detection kit
    A Flow cytometry analysis of neutrophil <t>apoptosis</t> after coculture with sodium decanoate (DeaNa), sodium palmitate (PaNa), and PBS. Quantitative graphs showing the proportion of late apoptotic/necrotic <t>(Annexin</t> V⁺/PI⁺) neutrophils ( B ) and early apoptotic (Annexin V⁺/PI⁻) neutrophils ( C ) under different treatments ( n = 3). D Schematic representation of neutrophil migration in vitro for 3 h at 37 °C. Created in BioRender. Yang, Q. (2026) https://BioRender.com/fm3t1j5 . Representative images of migrated neutrophils on the lower membrane stained with DAPI and photographed using the multifunctional enzyme reader. E Numbers of migrating neutrophils ( n = 3). F Volcano plot of differential gene expression in sodium decanoate-treated and PBS-treated neutrophils. G Heatmap showing the DEGs identified via RNA-seq in the presence of decanoic acid or PBS. H Quantitative reverse transcription PCR (qRT‒PCR) validation of gene expression changes in neutrophils treated with decanoic acid, palmitic acid and PBS ( n = 4). I Cell supernatant CXCL8 expression in neutrophils treated with decanoic acid, palmitic acid and PBS, as determined by ELISA ( n = 4). J Simplified schematic illustrating the coculture assay of neutrophils with Caco-2 cells, detailing the experimental setup. Created in BioRender. Yang, Q. (2026) https://BioRender.com/74ys0y4 . K qRT‒PCR validation of gene expression changes in Caco-2 cells after coculture with different fatty-acid-treated neutrophils ( n = 3). Data are shown as mean ± SEM, with representative results from at least three independent experiments. P values were determined by one-way ANOVA with Holm–Šidák test ( B , C ), one-way ANOVA with Dunnett’s test ( E , K ), one-way ANOVA with Tukey test ( H – I ). DeaNa, sodium decanoate; PaNa, sodium palmitate. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns P > 0.05. Exact P values: B DeaNa vs PBS, P = 0.0465, PaNa vs PBS, P = 0.0002; C DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P < 0.0001; E DeaNa vs PBS, P = 0.0342, PaNa vs PBS, P = 0.4726; H Cxcl2 , DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0001; Cxcl8 , DeaNa vs PBS, P = 0.0120, PaNa vs PBS, P = 0.0725; Cpt1a , DeaNa vs PBS, P = 0.0441, PaNa vs PBS, P < 0.0001; Osm , DeaNa vs PBS, P = 0.0078, PaNa vs PBS, P = 0.0019; Vegfa , DeaNa vs PBS, P = 0.0319, PaNa vs PBS, P = 0.0009. I DeaNa vs PBS, P = 0.0016, PaNa vs PBS, P = 0.0101. K CXCL8, DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P = 0.9138; OSM, DeaNa vs PBS, P = 0.0865, PaNa vs PBS, P = 0.0031; VEGFA, DeaNa vs PBS, P = 0.0053, PaNa vs PBS, P < 0.0001; AREG, DeaNa vs PBS, P = 0.0451, PaNa vs PBS, P = 0.0391; PTGS2, DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0224.
    Annexin V Fitc Propidium Iodide Pi Pe Apoptosis Detection Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/annexin+v+fitc+pi+apoptosis+detection+kit/Annexin+A5%2FANXA5%2C+Human/pmc13443604-502-9-16
    Average 95 stars, based on 26 article reviews
    annexin v fitc propidium iodide pi pe apoptosis detection kit - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Fusobacterium periodonticum promotes colorectal tumorigenesis via decanoic acid-driven neutrophil chemotaxis"

    Article Title: Fusobacterium periodonticum promotes colorectal tumorigenesis via decanoic acid-driven neutrophil chemotaxis

    Journal: Nature Communications

    doi: 10.1038/s41467-026-74591-y

    A Flow cytometry analysis of neutrophil apoptosis after coculture with sodium decanoate (DeaNa), sodium palmitate (PaNa), and PBS. Quantitative graphs showing the proportion of late apoptotic/necrotic (Annexin V⁺/PI⁺) neutrophils ( B ) and early apoptotic (Annexin V⁺/PI⁻) neutrophils ( C ) under different treatments ( n = 3). D Schematic representation of neutrophil migration in vitro for 3 h at 37 °C. Created in BioRender. Yang, Q. (2026) https://BioRender.com/fm3t1j5 . Representative images of migrated neutrophils on the lower membrane stained with DAPI and photographed using the multifunctional enzyme reader. E Numbers of migrating neutrophils ( n = 3). F Volcano plot of differential gene expression in sodium decanoate-treated and PBS-treated neutrophils. G Heatmap showing the DEGs identified via RNA-seq in the presence of decanoic acid or PBS. H Quantitative reverse transcription PCR (qRT‒PCR) validation of gene expression changes in neutrophils treated with decanoic acid, palmitic acid and PBS ( n = 4). I Cell supernatant CXCL8 expression in neutrophils treated with decanoic acid, palmitic acid and PBS, as determined by ELISA ( n = 4). J Simplified schematic illustrating the coculture assay of neutrophils with Caco-2 cells, detailing the experimental setup. Created in BioRender. Yang, Q. (2026) https://BioRender.com/74ys0y4 . K qRT‒PCR validation of gene expression changes in Caco-2 cells after coculture with different fatty-acid-treated neutrophils ( n = 3). Data are shown as mean ± SEM, with representative results from at least three independent experiments. P values were determined by one-way ANOVA with Holm–Šidák test ( B , C ), one-way ANOVA with Dunnett’s test ( E , K ), one-way ANOVA with Tukey test ( H – I ). DeaNa, sodium decanoate; PaNa, sodium palmitate. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns P > 0.05. Exact P values: B DeaNa vs PBS, P = 0.0465, PaNa vs PBS, P = 0.0002; C DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P < 0.0001; E DeaNa vs PBS, P = 0.0342, PaNa vs PBS, P = 0.4726; H Cxcl2 , DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0001; Cxcl8 , DeaNa vs PBS, P = 0.0120, PaNa vs PBS, P = 0.0725; Cpt1a , DeaNa vs PBS, P = 0.0441, PaNa vs PBS, P < 0.0001; Osm , DeaNa vs PBS, P = 0.0078, PaNa vs PBS, P = 0.0019; Vegfa , DeaNa vs PBS, P = 0.0319, PaNa vs PBS, P = 0.0009. I DeaNa vs PBS, P = 0.0016, PaNa vs PBS, P = 0.0101. K CXCL8, DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P = 0.9138; OSM, DeaNa vs PBS, P = 0.0865, PaNa vs PBS, P = 0.0031; VEGFA, DeaNa vs PBS, P = 0.0053, PaNa vs PBS, P < 0.0001; AREG, DeaNa vs PBS, P = 0.0451, PaNa vs PBS, P = 0.0391; PTGS2, DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0224.
    Figure Legend Snippet: A Flow cytometry analysis of neutrophil apoptosis after coculture with sodium decanoate (DeaNa), sodium palmitate (PaNa), and PBS. Quantitative graphs showing the proportion of late apoptotic/necrotic (Annexin V⁺/PI⁺) neutrophils ( B ) and early apoptotic (Annexin V⁺/PI⁻) neutrophils ( C ) under different treatments ( n = 3). D Schematic representation of neutrophil migration in vitro for 3 h at 37 °C. Created in BioRender. Yang, Q. (2026) https://BioRender.com/fm3t1j5 . Representative images of migrated neutrophils on the lower membrane stained with DAPI and photographed using the multifunctional enzyme reader. E Numbers of migrating neutrophils ( n = 3). F Volcano plot of differential gene expression in sodium decanoate-treated and PBS-treated neutrophils. G Heatmap showing the DEGs identified via RNA-seq in the presence of decanoic acid or PBS. H Quantitative reverse transcription PCR (qRT‒PCR) validation of gene expression changes in neutrophils treated with decanoic acid, palmitic acid and PBS ( n = 4). I Cell supernatant CXCL8 expression in neutrophils treated with decanoic acid, palmitic acid and PBS, as determined by ELISA ( n = 4). J Simplified schematic illustrating the coculture assay of neutrophils with Caco-2 cells, detailing the experimental setup. Created in BioRender. Yang, Q. (2026) https://BioRender.com/74ys0y4 . K qRT‒PCR validation of gene expression changes in Caco-2 cells after coculture with different fatty-acid-treated neutrophils ( n = 3). Data are shown as mean ± SEM, with representative results from at least three independent experiments. P values were determined by one-way ANOVA with Holm–Šidák test ( B , C ), one-way ANOVA with Dunnett’s test ( E , K ), one-way ANOVA with Tukey test ( H – I ). DeaNa, sodium decanoate; PaNa, sodium palmitate. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns P > 0.05. Exact P values: B DeaNa vs PBS, P = 0.0465, PaNa vs PBS, P = 0.0002; C DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P < 0.0001; E DeaNa vs PBS, P = 0.0342, PaNa vs PBS, P = 0.4726; H Cxcl2 , DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0001; Cxcl8 , DeaNa vs PBS, P = 0.0120, PaNa vs PBS, P = 0.0725; Cpt1a , DeaNa vs PBS, P = 0.0441, PaNa vs PBS, P < 0.0001; Osm , DeaNa vs PBS, P = 0.0078, PaNa vs PBS, P = 0.0019; Vegfa , DeaNa vs PBS, P = 0.0319, PaNa vs PBS, P = 0.0009. I DeaNa vs PBS, P = 0.0016, PaNa vs PBS, P = 0.0101. K CXCL8, DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P = 0.9138; OSM, DeaNa vs PBS, P = 0.0865, PaNa vs PBS, P = 0.0031; VEGFA, DeaNa vs PBS, P = 0.0053, PaNa vs PBS, P < 0.0001; AREG, DeaNa vs PBS, P = 0.0451, PaNa vs PBS, P = 0.0391; PTGS2, DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0224.

    Techniques Used: Flow Cytometry, Migration, In Vitro, Membrane, Staining, Gene Expression, RNA Sequencing, Reverse Transcription, Biomarker Discovery, Expressing, Enzyme-linked Immunosorbent Assay, Co-culture Assay

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    Binding Assay:

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    Staining:

    Article Title: Pterostilbene ameliorates benzo[b]fluoranthene-induced lung injury by suppressing Toll-like receptor 4/nuclear factor kappa-B pathway and mitochondria-mediated apoptosis.
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    Article Title: Fusobacterium periodonticum promotes colorectal tumorigenesis via decanoic acid-driven neutrophil chemotaxis
    Article Snippet: .. After treatment, cells were collected and stained using an Annexin V-FITC/Propidium Iodide (PI)-PE Apoptosis Detection Kit (MedChemExpress, USA). ..

    Incubation:

    Article Title: Pterostilbene ameliorates benzo[b]fluoranthene-induced lung injury by suppressing Toll-like receptor 4/nuclear factor kappa-B pathway and mitochondria-mediated apoptosis.
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    Control:

    Article Title: Scalable longitudinal imaging and transcriptomics of cells in dynamic enclosures
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    RNA Extraction:

    Article Title: RFWD2 Mitigates AD ‐Like Cognitive Impairments via the JNK – SGK1 Signaling Pathway in Mice
    Article Snippet: .. The following is a list of all key reagents used in this study: DMEM basic (Gibco, Waltham, MA, USA), neurobasal medium [−] l ‐glutamine (Gibco), B‐27 serum‐free supplement (Gibco), GlutaMAX (Gibco), fetal bovine serum (Gibco), antibiotic‐antimycotic (Gibco), 0.25% EDTA‐containing phenol red trypsin (1 ×) (Gibco), poly‐ d ‐lysine (PDL) (Sigma‐Aldrich, St. Louis, MO, USA), SP600125 (MedChemExpress, Monmouth Junction, NJ, USA), GSK650394 (MedChemExpress), phosphatase inhibitor cocktail (Bioss, Woburn, MA, USA), RNA Extraction Kit (Vazyme, Nanjing, China), TUNEL (Cat. No: HY‐K1078; MedChemExpress), 2′, 7′‐dichlorodihydrofluorescein diacetate fluorescent probe (Cat: HY‐D0940; MedChemExpress), Annexin‐V‐FITC (Cat. No: HY‐K1073; MedChemExpress), Mitochondrial Membrane Potential Detection Kit (Beyotime Biotechnology, Shanghai, China), mouse monoclonal anti‐RFWD2 (Sigma‐Aldrich), rabbit polyclonal anti‐SGK1 (Wuhan Proteintech, Wuhan China), rabbit polyclonal anti‐phosphorylated JNK ( p ‐JNK) (Santa Cruz Biotechnology, Dallas, TX, USA), mouse polyclonal anti‐p‐Tau (Santa Cruz Biotechnology), rabbit polyclonal anti‐Kv1.4 (Invitrogen, Waltham, MA, USA), mouse monoclonal anti‐MAP‐2 (Santa Cruz Biotechnology), mouse polyclonal anti‐p‐JNK (Santa Cruz Biotechnology), rabbit polyclonal anti‐JNK3 (Abcam, Cambridge, UK), donkey anti‐rabbit Cy3 (The Jackson Laboratory, Bar Harbor, ME, USA), donkey anti‐mouse Alexa Fluor 488 (Invitrogen), Anti‐Fluorescence Quenching Mounting Medium (Invitrogen), PAGE Gel Rapid Preparation Kit (Yeasen Biotechnology, Shanghai, China), and bovine serum albumin (full component) (Solarbio, Beijing, China). .. The gene sequence (CTCAACTCCTACGAGGACAAA) of the previously verified effective RFWD2‐specific shRNA was obtained from the NCBI website, and the LV‐RFWD2‐shRNA‐mCherry lentivirus was constructed by GenePharma (Suzhou, China).

    TUNEL Assay:

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    Membrane:

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    Article Snippet: .. The following is a list of all key reagents used in this study: DMEM basic (Gibco, Waltham, MA, USA), neurobasal medium [−] l ‐glutamine (Gibco), B‐27 serum‐free supplement (Gibco), GlutaMAX (Gibco), fetal bovine serum (Gibco), antibiotic‐antimycotic (Gibco), 0.25% EDTA‐containing phenol red trypsin (1 ×) (Gibco), poly‐ d ‐lysine (PDL) (Sigma‐Aldrich, St. Louis, MO, USA), SP600125 (MedChemExpress, Monmouth Junction, NJ, USA), GSK650394 (MedChemExpress), phosphatase inhibitor cocktail (Bioss, Woburn, MA, USA), RNA Extraction Kit (Vazyme, Nanjing, China), TUNEL (Cat. No: HY‐K1078; MedChemExpress), 2′, 7′‐dichlorodihydrofluorescein diacetate fluorescent probe (Cat: HY‐D0940; MedChemExpress), Annexin‐V‐FITC (Cat. No: HY‐K1073; MedChemExpress), Mitochondrial Membrane Potential Detection Kit (Beyotime Biotechnology, Shanghai, China), mouse monoclonal anti‐RFWD2 (Sigma‐Aldrich), rabbit polyclonal anti‐SGK1 (Wuhan Proteintech, Wuhan China), rabbit polyclonal anti‐phosphorylated JNK ( p ‐JNK) (Santa Cruz Biotechnology, Dallas, TX, USA), mouse polyclonal anti‐p‐Tau (Santa Cruz Biotechnology), rabbit polyclonal anti‐Kv1.4 (Invitrogen, Waltham, MA, USA), mouse monoclonal anti‐MAP‐2 (Santa Cruz Biotechnology), mouse polyclonal anti‐p‐JNK (Santa Cruz Biotechnology), rabbit polyclonal anti‐JNK3 (Abcam, Cambridge, UK), donkey anti‐rabbit Cy3 (The Jackson Laboratory, Bar Harbor, ME, USA), donkey anti‐mouse Alexa Fluor 488 (Invitrogen), Anti‐Fluorescence Quenching Mounting Medium (Invitrogen), PAGE Gel Rapid Preparation Kit (Yeasen Biotechnology, Shanghai, China), and bovine serum albumin (full component) (Solarbio, Beijing, China). .. The gene sequence (CTCAACTCCTACGAGGACAAA) of the previously verified effective RFWD2‐specific shRNA was obtained from the NCBI website, and the LV‐RFWD2‐shRNA‐mCherry lentivirus was constructed by GenePharma (Suzhou, China).

    Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis
    Article Snippet: Lastly, the cells were examined using a fluorescence microscope (Olympus) and images were captured. .. Double staining with Annexin V-Alexa Fluor 647 (Yeasen) and tetramethylrhodamine (abbreviation as TMRE; MCE, Shanghai, China) was used to measure the changes in apoptosis and mitochondrial membrane potential. ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: RFWD2 Mitigates AD ‐Like Cognitive Impairments via the JNK – SGK1 Signaling Pathway in Mice
    Article Snippet: .. The following is a list of all key reagents used in this study: DMEM basic (Gibco, Waltham, MA, USA), neurobasal medium [−] l ‐glutamine (Gibco), B‐27 serum‐free supplement (Gibco), GlutaMAX (Gibco), fetal bovine serum (Gibco), antibiotic‐antimycotic (Gibco), 0.25% EDTA‐containing phenol red trypsin (1 ×) (Gibco), poly‐ d ‐lysine (PDL) (Sigma‐Aldrich, St. Louis, MO, USA), SP600125 (MedChemExpress, Monmouth Junction, NJ, USA), GSK650394 (MedChemExpress), phosphatase inhibitor cocktail (Bioss, Woburn, MA, USA), RNA Extraction Kit (Vazyme, Nanjing, China), TUNEL (Cat. No: HY‐K1078; MedChemExpress), 2′, 7′‐dichlorodihydrofluorescein diacetate fluorescent probe (Cat: HY‐D0940; MedChemExpress), Annexin‐V‐FITC (Cat. No: HY‐K1073; MedChemExpress), Mitochondrial Membrane Potential Detection Kit (Beyotime Biotechnology, Shanghai, China), mouse monoclonal anti‐RFWD2 (Sigma‐Aldrich), rabbit polyclonal anti‐SGK1 (Wuhan Proteintech, Wuhan China), rabbit polyclonal anti‐phosphorylated JNK ( p ‐JNK) (Santa Cruz Biotechnology, Dallas, TX, USA), mouse polyclonal anti‐p‐Tau (Santa Cruz Biotechnology), rabbit polyclonal anti‐Kv1.4 (Invitrogen, Waltham, MA, USA), mouse monoclonal anti‐MAP‐2 (Santa Cruz Biotechnology), mouse polyclonal anti‐p‐JNK (Santa Cruz Biotechnology), rabbit polyclonal anti‐JNK3 (Abcam, Cambridge, UK), donkey anti‐rabbit Cy3 (The Jackson Laboratory, Bar Harbor, ME, USA), donkey anti‐mouse Alexa Fluor 488 (Invitrogen), Anti‐Fluorescence Quenching Mounting Medium (Invitrogen), PAGE Gel Rapid Preparation Kit (Yeasen Biotechnology, Shanghai, China), and bovine serum albumin (full component) (Solarbio, Beijing, China). .. The gene sequence (CTCAACTCCTACGAGGACAAA) of the previously verified effective RFWD2‐specific shRNA was obtained from the NCBI website, and the LV‐RFWD2‐shRNA‐mCherry lentivirus was constructed by GenePharma (Suzhou, China).

    Double Staining:

    Article Title: CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis
    Article Snippet: Lastly, the cells were examined using a fluorescence microscope (Olympus) and images were captured. .. Double staining with Annexin V-Alexa Fluor 647 (Yeasen) and tetramethylrhodamine (abbreviation as TMRE; MCE, Shanghai, China) was used to measure the changes in apoptosis and mitochondrial membrane potential. ..



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