resource source identifier antibodies mouse monoclonal anti heparan sulfate 10e4 amsbio rrid ab 10013601 goat anti mouse igg (AMS Biotechnology)
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Resource Source Identifier Antibodies Mouse Monoclonal Anti Heparan Sulfate 10e4 Amsbio Rrid Ab 10013601 Goat Anti Mouse Igg, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ams%2Egag-hs01/Heparan+Sulfate/pm41275491-273-2-11
Average 96 stars, based on 102 article reviews
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Staining:Article Title: Saffold virus exploits integrin αvβ8 and sulfated glycosaminoglycans as cooperative attachment receptors for infection Article Snippet: The cells were analyzed using a FACS Canto II (BD Biosciences) or a DxFLEX (Beckman Coulter) flow cytometer, and data were processed using WinMDI or Kaluza (Beckman Coulter) software. .. To detect HS, the cells were stained with Article Title: Saffold virus exploits integrin αvβ8 and sulfated glycosaminoglycans as cooperative attachment receptors for infection. Article Snippet: The cells were analyzed using a FACS Canto II (BD Biosciences) or a DxFLEX (Beckman Coulter) flow cytometer, and data were processed using WinMDI or Kaluza (Beckman Coulter) software. .. To detect HS, the cells were stained with Article Title: Enhanced lysosomal exocytosis and altered growth factor signaling are associated with cartilage pathology in a model of mucopolysaccharidosis type IVA Article Snippet: 20 μm sections were cut on a Leica cryostat. .. Primary antibodies used for staining included an anti-GFP antibody (1:100; 31 A-21311, Thermo Fisher Scientific), an Control:Article Title: Saffold virus exploits integrin αvβ8 and sulfated glycosaminoglycans as cooperative attachment receptors for infection Article Snippet: The cells were analyzed using a FACS Canto II (BD Biosciences) or a DxFLEX (Beckman Coulter) flow cytometer, and data were processed using WinMDI or Kaluza (Beckman Coulter) software. .. To detect HS, the cells were stained with Article Title: Saffold virus exploits integrin αvβ8 and sulfated glycosaminoglycans as cooperative attachment receptors for infection. Article Snippet: The cells were analyzed using a FACS Canto II (BD Biosciences) or a DxFLEX (Beckman Coulter) flow cytometer, and data were processed using WinMDI or Kaluza (Beckman Coulter) software. .. To detect HS, the cells were stained with Incubation:Article Title: Endoplasmic reticulum stress induced autophagy alters cellular processing of cationic lipid delivered siRNAs. Article Snippet: Complexes were made to achieve a final concentration of 1.75 μg/mL LF2K (Invitrogen, 11,668,019) and 150 nM siRNA (Horizon Discovery, Guide strand sequence: GGC UAC GUC CAG GAG CGC AUU) in 50 μL of Opti-MEM (Gibco, 31,985,062) and 500 μL of DMEM. .. Cells were incubated with the transfection solution for 4 h and then washed with 50 μg/mL Article Title: Early Transcriptomic Response of Human Iris Stromal Cells During Herpes Simplex Virus Entry Reveals Interplay Between Cell Glycocalyx and Viral Exploitation Article Snippet: Samples were then blocked using 1% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) in PBS with 0.2% Tween-20 (PBS-T). .. After blocking, coverslips were washed again and incubated with Article Title: Two-photon microscopy of brain endothelial glycocalyx uncovers spatial heterogeneity, vesicular transport, and lectin-binding kinetics in the living brain Article Snippet: .. Brain sections were incubated with primary antibody diluted in 2.5% BSA in PBS ( Article Title: ADAM10 tailors extracellular vesicles for content transfer rather than signaling by contact Article Snippet: Antibodies directed against SDC1 intracellular domain (D4Y7H) was from (cell signaling #12922, dilution 1/1000), GFP (A11122) from Thermofisher (dilution 1/1000), ADAM17 (abcam, #ab39162, 1/1000 or cell signaling #3976, 1/1000), ADAM10 (abcam, #ab1997, 1/1 000), GAPDH (Proteintech # 10494-1-AP), Flotillin-1 (BD Biosciences, #X11669), Ephrin B3 (santa cruz, # sc-514139), PTK7 (Proteintech, #17799), E-cadherin (R&D Systems, #AF748), BCAM (R&D Systems, #BAF148), STAT3 124H6 (cell signaling #9139), p-STAT3 (cell signaling #9131). .. To enzymatically digest the glycosaminoglycans (GAGs) on SDC, including Article Title: Early Transcriptomic Response of Human Iris Stromal Cells During Herpes Simplex Virus Entry Reveals Interplay Between Cell Glycocalyx and Viral Exploitation Article Snippet: Samples were then blocked using 1% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) in PBS with 0.2% Tween-20 (PBS-T). .. After blocking, coverslips were washed again and incubated with Transfection:Article Title: Endoplasmic reticulum stress induced autophagy alters cellular processing of cationic lipid delivered siRNAs. Article Snippet: Complexes were made to achieve a final concentration of 1.75 μg/mL LF2K (Invitrogen, 11,668,019) and 150 nM siRNA (Horizon Discovery, Guide strand sequence: GGC UAC GUC CAG GAG CGC AUU) in 50 μL of Opti-MEM (Gibco, 31,985,062) and 500 μL of DMEM. .. Cells were incubated with the transfection solution for 4 h and then washed with 50 μg/mL Blocking Assay:Article Title: Early Transcriptomic Response of Human Iris Stromal Cells During Herpes Simplex Virus Entry Reveals Interplay Between Cell Glycocalyx and Viral Exploitation Article Snippet: Samples were then blocked using 1% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) in PBS with 0.2% Tween-20 (PBS-T). .. After blocking, coverslips were washed again and incubated with Article Title: Early Transcriptomic Response of Human Iris Stromal Cells During Herpes Simplex Virus Entry Reveals Interplay Between Cell Glycocalyx and Viral Exploitation Article Snippet: Samples were then blocked using 1% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) in PBS with 0.2% Tween-20 (PBS-T). .. After blocking, coverslips were washed again and incubated with Concentration Assay:Article Title: ADAM10 tailors extracellular vesicles for content transfer rather than signaling by contact Article Snippet: Antibodies directed against SDC1 intracellular domain (D4Y7H) was from (cell signaling #12922, dilution 1/1000), GFP (A11122) from Thermofisher (dilution 1/1000), ADAM17 (abcam, #ab39162, 1/1000 or cell signaling #3976, 1/1000), ADAM10 (abcam, #ab1997, 1/1 000), GAPDH (Proteintech # 10494-1-AP), Flotillin-1 (BD Biosciences, #X11669), Ephrin B3 (santa cruz, # sc-514139), PTK7 (Proteintech, #17799), E-cadherin (R&D Systems, #AF748), BCAM (R&D Systems, #BAF148), STAT3 124H6 (cell signaling #9139), p-STAT3 (cell signaling #9131). .. To enzymatically digest the glycosaminoglycans (GAGs) on SDC, including |
