anti ngf mouse monoclonal blocking antibody  (Alomone Labs)

Journal: Cell reports

Article Title: RIP2 Gates TRAF6 Interaction with Death Receptor p75 NTR to Regulate Cerebellar Granule Neuron Survival.

doi: 10.1016/j.celrep.2018.06.098

Figure Lengend Snippet: Figure 1. Expression of p75NTR, NGF, and RIP2 during Postnatal Cerebellar Develop- ment (A–C) p75NTR mRNA (A), Ngf mRNA (B), and ripk2 mRNA (C) quantification relative to 18S RNA levels in cerebellum at the indicated postnatal (P) ages (mean ± SEM of data from 5 cerebella at each age). (D–F) Representative immunoblots and quantifi- cation of p75NTR (D) mature (mNGF), proNGF (E), and RIP2 (F) protein in total cerebellar protein extracts at the indicated ages (mean ± SEM densitometry from six experiments; representative blots are shown). (G) Micrographs of representative P7 cerebellum sections labeled with RIP2 antisense in situ hybridization probe, anti-p75NTR antibody, and counterstained with DAPI. The scale bars repre- sent 50 mm. (H) Immunoblots showing the expression of p75NTR and RIP2 in CGNs cultured for the indi- cated days in vitro (DIV).

Article Snippet: Mature NGF (N-100), brain-derived neurotrophic factor (BDNF) (B-250), and anti-NGF/ proNGF (total NGF) neutralizing antibody (ALM-006) were obtained from Alomone.

Techniques: Expressing, Western Blot, Labeling, In Situ Hybridization, Cell Culture, In Vitro