aga 004 rrid ab 203986 8 calbindin rabbit  (Alomone Labs)

Journal: IBRO Neuroscience Reports

Article Title: Mapping the effect of the antisecretory factor on GABA A receptor α 1 and α 6 subunits in cerebellar granule cells in vitro

doi: 10.1016/j.ibneur.2024.08.001

Figure Lengend Snippet: 1.0 µM AF-16 percentage effect for α 6 - (magenta) and α 1 -containing GABA A Rs (blue) in non-permeabilized granule cell cultures. Vertical bars represent SEMs. Student’s t-test evaluated E% statistical significance: ***p < 0.001, n.s. is not significant.

Article Snippet: The primary antibodies used were as follows: - Polyclonal rabbit antibody against rat GABA A receptor α 6 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA; - Polyclonal rabbit antibody against rat GABA A receptor α 1 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: Mapping the effect of the antisecretory factor on GABA A receptor α 1 and α 6 subunits in cerebellar granule cells in vitro

doi: 10.1016/j.ibneur.2024.08.001

Figure Lengend Snippet: 1.0 µM AF-16 percentage effect for α 6 - (magenta) and α 1 -containing GABA A Rs (blue) in permeabilized granule cell cultures. Vertical bars represent SEMs. Student’s t-test evaluated E% statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, n.s. is not significant.

Article Snippet: The primary antibodies used were as follows: - Polyclonal rabbit antibody against rat GABA A receptor α 6 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA; - Polyclonal rabbit antibody against rat GABA A receptor α 1 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: Mapping the effect of the antisecretory factor on GABA A receptor α 1 and α 6 subunits in cerebellar granule cells in vitro

doi: 10.1016/j.ibneur.2024.08.001

Figure Lengend Snippet: Statistical significance, calculated with Student’s t-test, of the results for soma-neurite in non-permeabilized and permeabilized samples for α 1 and α 6 -containing GABA A Rs.

Article Snippet: The primary antibodies used were as follows: - Polyclonal rabbit antibody against rat GABA A receptor α 6 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA; - Polyclonal rabbit antibody against rat GABA A receptor α 1 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: Mapping the effect of the antisecretory factor on GABA A receptor α 1 and α 6 subunits in cerebellar granule cells in vitro

doi: 10.1016/j.ibneur.2024.08.001

Figure Lengend Snippet: Statistical significance, calculated with Student’s t-test, for non-permeabilized and permeabilized samples either for α 1 and α 6 subunits in soma or neurite. n.s. is not significant.

Article Snippet: The primary antibodies used were as follows: - Polyclonal rabbit antibody against rat GABA A receptor α 6 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA; - Polyclonal rabbit antibody against rat GABA A receptor α 1 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: Mapping the effect of the antisecretory factor on GABA A receptor α 1 and α 6 subunits in cerebellar granule cells in vitro

doi: 10.1016/j.ibneur.2024.08.001

Figure Lengend Snippet: 3D-STED reconstruction of permeabilized cerebellar granule cells labeled with primary antibodies anti-α 6 GABA A receptor and ATTO594 secondary antibodies. The Z-stacks were acquired at 512 × 512 pixels, 47.68 μm × 47.68 μm, 16-bit, 200 Hz, 2-line averages, 0.070 μm Z-step size, 1.0 Airy disk. A shows the control samples; B shows 1 h treated samples with 1.0 μM AF-16.

Article Snippet: The primary antibodies used were as follows: - Polyclonal rabbit antibody against rat GABA A receptor α 6 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA; - Polyclonal rabbit antibody against rat GABA A receptor α 1 subunit (Alomone Labs Ltd., Israel) at a concentration of 1:500 in 2 % BSA.

Techniques: Labeling, Control

Journal: eLife

Article Title: GluA4 facilitates cerebellar expansion coding and enables associative memory formation

doi: 10.7554/eLife.65152

Figure Lengend Snippet: High-speed (400 fps) video of a mouse crossing the LocoMouse corridor, displayed at 30 fps. Side and bottom (via mirror reflection) views of the mouse are captured in a single camera. Top: Raw video of a WT mouse. Bottom: Raw video of a GluA4-knockout (GluA4-KO) mouse.

Article Snippet: Antibody , Anti-GABA A R-α6 (rabbit polyclonal) , Alomone , Cat. # AGA-004 RRID: AB_2039868 , WB (1:500).

Techniques:

Journal: Acta Pharmaceutica Sinica. B

Article Title: Deficiency of anti-inflammatory cytokine IL-4 leads to neural hyperexcitability and aggravates cerebral ischemia–reperfusion injury

doi: 10.1016/j.apsb.2020.05.002

Figure Lengend Snippet: Upregulation of Nav1.1 and downregulations of KCa3.1 and α 6 subunit of GABA A receptors in the cortex from Il-4 −/− mice and supplemental IL-4 increases KCa3.1 and α 6 mRNA expressions. Upregulation of Nav1.1 mRNA expression and downregulations of KCa3.1 and α 6 subunit of GABA A receptors mRNA expression, in cortical tissues (A) and cortical neurons (B) from Il-4 −/− mice. (C) Nav1.1 protein expression in primary mouse cortical neurons by immunostaining and upregulation of Nav1.1 protein in Il-4 −/− mice ( n = 6 mice). (D) The image staining with KCa3.1 antibody (green), NeuN antibody (red, a neuronal-specific nucleus marker) and DAPI (blue, a nucleus marker). Downregulation of KCa3.1 protein in Il-4 −/− mice ( n = 4 mice, Mann Whitney test). (E) Downregulation of α 6 subunit of GABA A protein in Il-4 −/− mice ( n = 4 mice). Increased mRNA expressions of KCa3.1 and α 6 subunit in Il-4 −/− (F) and Il-4 +/+ (G) cortical neurons after supplementing IL-4 (20 ng/mL) in culture for 7 days. Data are expressed as the mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their controls. The numbers at the bottom of the bars indicate the number of repeats or mice in the group.

Article Snippet: After blocking by 5% milk, PVDF membranes were incubated with primary antibodies at 4 °C overnight such as rat anti-IL-4 antibody (1:500, Abcam, ab11524), mouse monoclonal anti-KCa3.1 antibody (1:250, Alomone, ALM-051), rabbit anti-Nav1.1 antibody (1:250, Alomone, ASC-001), rabbit GABA A R α 6 Polyclonal Antibody (1:200, Alomone, AGA-004), mouse anti- β -actin antibody (1:5000, Abcam) and mouse anti-GAPDH antibody (1:5000, Abcam).

Techniques: Expressing, Immunostaining, Staining, Marker, MANN-WHITNEY, Real-time Polymerase Chain Reaction, Western Blot, Immunofluorescence

Journal: Acta Pharmaceutica Sinica. B

Article Title: Deficiency of anti-inflammatory cytokine IL-4 leads to neural hyperexcitability and aggravates cerebral ischemia–reperfusion injury

doi: 10.1016/j.apsb.2020.05.002

Figure Lengend Snippet: A proposed molecular mechanism underlying increased neural excitabilities and susceptibility to ischemic injury caused by IL-4 deficiency. IL-4 binding to IL-4R actives IL-4 pathway. IL-4 deficiency alters gene transcriptions by downregulating the Kcnn4 gene encoding KCa3.1 protein and Gabra6 gene encoding GABA A receptor chloride channel and upregulating the Scna1 gene encoding Nav1.1 protein through IL-4 signaling pathways. Downregulation of KCa3.1 channels and tonic GABA A receptors can reduce potassium outflow and chloride inflow in neurons, leading to enhanced neuronal firings through membrane depolarization. The upregulation of Nav1.1 channels can increase sodium inflow in neurons. All these alterations can enhance neuronal hyperexcitability and glutamate release from excitatory axon terminals, ultimately increasing susceptibility to ischemic injury. Conversely, enhancement of IL-4 signaling through supplemental IL-4 can increase KCa3.1 and α 6 subunit of GABA A receptors in cortical neurons and reverse neuronal hyperexcitability, thus exerting neuroprotection against ischemic injury.

Article Snippet: After blocking by 5% milk, PVDF membranes were incubated with primary antibodies at 4 °C overnight such as rat anti-IL-4 antibody (1:500, Abcam, ab11524), mouse monoclonal anti-KCa3.1 antibody (1:250, Alomone, ALM-051), rabbit anti-Nav1.1 antibody (1:250, Alomone, ASC-001), rabbit GABA A R α 6 Polyclonal Antibody (1:200, Alomone, AGA-004), mouse anti- β -actin antibody (1:5000, Abcam) and mouse anti-GAPDH antibody (1:5000, Abcam).

Techniques: Binding Assay, Protein-Protein interactions, Membrane